Matches in SemOpenAlex for { <https://semopenalex.org/work/W2597843606> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2597843606 abstract "636 Background: Standard treatment for metastatic colorectal cancer (mCRC) is systemic chemotherapy with anti-EGFR treatment, depending on KRAS mutational status. However, tumors harboring a KRAS mutation do not respond to existing targeted therapy. Moreover, targeting mutant KRAS has, to date, not been possible. Herein, we explore using a KRAS inhibitory nanoparticle (NP), to directly knock down mutant KRAS. Methods: Utilizing fluorescent-labeled small interfering RNA (siRNA) NPs, uptake was assessed via fluorescent microscopy. KRAS mutant CT26 and wild-type MC38 CRC cell lines were incubated with either scramble (Sc) sequence siRNA NP, KRAS siRNA NP, or FOLFOX (fluorouracil + oxaliplatin) chemotherapy ± KRAS siRNA NP. Cell viability was assessed via a luminescent viability assay. KRAS and cleaved caspase 3 protein expression were assessed using western blotting. Results: Fluorescent NP uptake was demonstrated in CT26 cells as early as 260 minutes post treatment, with increased uptake through 780 minutes. Decreased cellular viability was seen with KRAS siRNA NP treated CT26 cells, as compared to both Sc siRNA NP and non-treated CT26 cells (both p < 0.0001). Cell viability was significantly diminished with FOLFOX combined with KRAS siRNA NP as compared to FOLFOX alone for CT26 cells ( p = 0.0003), but not MC38 cells (p = 0.2259). Western blot demonstrated decreased KRAS and increased cleaved caspase 3 expression in CT26 cells treated with KRAS siRNA NP. Conclusions: A KRAS siRNA tagged NP was internalized by the CRC cells in vitro, and induced cellular death via apoptosis in mutant type KRAS CRC. In addition, KRAS siRNA NP acted synergistically with FOLFOX chemotherapy to enhance cell death. We believe KRAS inhibition based NP treatment is a promising target for mutant type KRAS CRC. [Table: see text]" @default.
- W2597843606 created "2017-04-07" @default.
- W2597843606 creator A5001895409 @default.
- W2597843606 creator A5008456176 @default.
- W2597843606 creator A5015591933 @default.
- W2597843606 creator A5044687253 @default.
- W2597843606 creator A5046404222 @default.
- W2597843606 creator A5062157417 @default.
- W2597843606 creator A5065615592 @default.
- W2597843606 creator A5081302342 @default.
- W2597843606 date "2017-02-01" @default.
- W2597843606 modified "2023-09-27" @default.
- W2597843606 title "Anti-KRAS siRNA nanoparticles for targeted colorectal cancer therapy." @default.
- W2597843606 doi "https://doi.org/10.1200/jco.2017.35.4_suppl.636" @default.
- W2597843606 hasPublicationYear "2017" @default.
- W2597843606 type Work @default.
- W2597843606 sameAs 2597843606 @default.
- W2597843606 citedByCount "1" @default.
- W2597843606 countsByYear W25978436062019 @default.
- W2597843606 crossrefType "journal-article" @default.
- W2597843606 hasAuthorship W2597843606A5001895409 @default.
- W2597843606 hasAuthorship W2597843606A5008456176 @default.
- W2597843606 hasAuthorship W2597843606A5015591933 @default.
- W2597843606 hasAuthorship W2597843606A5044687253 @default.
- W2597843606 hasAuthorship W2597843606A5046404222 @default.
- W2597843606 hasAuthorship W2597843606A5062157417 @default.
- W2597843606 hasAuthorship W2597843606A5065615592 @default.
- W2597843606 hasAuthorship W2597843606A5081302342 @default.
- W2597843606 hasConcept C121608353 @default.
- W2597843606 hasConcept C126322002 @default.
- W2597843606 hasConcept C1491633281 @default.
- W2597843606 hasConcept C153911025 @default.
- W2597843606 hasConcept C185592680 @default.
- W2597843606 hasConcept C22615655 @default.
- W2597843606 hasConcept C2776694085 @default.
- W2597843606 hasConcept C2778260052 @default.
- W2597843606 hasConcept C2780962732 @default.
- W2597843606 hasConcept C2781187634 @default.
- W2597843606 hasConcept C2781230642 @default.
- W2597843606 hasConcept C502942594 @default.
- W2597843606 hasConcept C526805850 @default.
- W2597843606 hasConcept C53227056 @default.
- W2597843606 hasConcept C54009773 @default.
- W2597843606 hasConcept C54355233 @default.
- W2597843606 hasConcept C55493867 @default.
- W2597843606 hasConcept C71924100 @default.
- W2597843606 hasConcept C81885089 @default.
- W2597843606 hasConcept C86803240 @default.
- W2597843606 hasConceptScore W2597843606C121608353 @default.
- W2597843606 hasConceptScore W2597843606C126322002 @default.
- W2597843606 hasConceptScore W2597843606C1491633281 @default.
- W2597843606 hasConceptScore W2597843606C153911025 @default.
- W2597843606 hasConceptScore W2597843606C185592680 @default.
- W2597843606 hasConceptScore W2597843606C22615655 @default.
- W2597843606 hasConceptScore W2597843606C2776694085 @default.
- W2597843606 hasConceptScore W2597843606C2778260052 @default.
- W2597843606 hasConceptScore W2597843606C2780962732 @default.
- W2597843606 hasConceptScore W2597843606C2781187634 @default.
- W2597843606 hasConceptScore W2597843606C2781230642 @default.
- W2597843606 hasConceptScore W2597843606C502942594 @default.
- W2597843606 hasConceptScore W2597843606C526805850 @default.
- W2597843606 hasConceptScore W2597843606C53227056 @default.
- W2597843606 hasConceptScore W2597843606C54009773 @default.
- W2597843606 hasConceptScore W2597843606C54355233 @default.
- W2597843606 hasConceptScore W2597843606C55493867 @default.
- W2597843606 hasConceptScore W2597843606C71924100 @default.
- W2597843606 hasConceptScore W2597843606C81885089 @default.
- W2597843606 hasConceptScore W2597843606C86803240 @default.
- W2597843606 hasLocation W25978436061 @default.
- W2597843606 hasOpenAccess W2597843606 @default.
- W2597843606 hasPrimaryLocation W25978436061 @default.
- W2597843606 hasRelatedWork W2031398056 @default.
- W2597843606 hasRelatedWork W2031925505 @default.
- W2597843606 hasRelatedWork W2054177711 @default.
- W2597843606 hasRelatedWork W2086479839 @default.
- W2597843606 hasRelatedWork W2173497597 @default.
- W2597843606 hasRelatedWork W2315491723 @default.
- W2597843606 hasRelatedWork W2333855314 @default.
- W2597843606 hasRelatedWork W2335996374 @default.
- W2597843606 hasRelatedWork W2417997339 @default.
- W2597843606 hasRelatedWork W2562170609 @default.
- W2597843606 hasRelatedWork W2562621608 @default.
- W2597843606 hasRelatedWork W2740242720 @default.
- W2597843606 hasRelatedWork W2810786408 @default.
- W2597843606 hasRelatedWork W2902674912 @default.
- W2597843606 hasRelatedWork W2955481428 @default.
- W2597843606 hasRelatedWork W2979976206 @default.
- W2597843606 hasRelatedWork W2989577472 @default.
- W2597843606 hasRelatedWork W2999667018 @default.
- W2597843606 hasRelatedWork W3180345462 @default.
- W2597843606 hasRelatedWork W3183471774 @default.
- W2597843606 isParatext "false" @default.
- W2597843606 isRetracted "false" @default.
- W2597843606 magId "2597843606" @default.
- W2597843606 workType "article" @default.