Matches in SemOpenAlex for { <https://semopenalex.org/work/W2598423465> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2598423465 endingPage "34" @default.
- W2598423465 startingPage "27" @default.
- W2598423465 abstract "Analysis of crustacean species plays a role in authenticity issues as well as allergen detection. A real-time PCR-based screening assay was developed for the detection of crustaceans in food. In order to cover most relevant species in one analytical step, PCR systems were newly developed for the detection of shrimps (Penaeidae), lobster (Homarus sp.), Common shrimp (Crangon crangon), river prawns (Macrobrachium sp.) and Chinese mitten crab (Eriocheir sinensis). In addition a published system targeting Northern prawn (Pandalus borealis) was selected. All PCR-systems are based on mitochondrial 16S rRNA gene sequences and were optimized to be run with a standard program at a universal annealing temperature of 60 °C. Validation experiments confirmed a sensitivity of the PCR systems of 0.01–0.1 genome copies or 0.04–2.5 pg DNA, respectively. Specificity was demonstrated with 25,000 copies of pure genomic DNA from about thirty plant and animal species relevant in food and the performance in food matrix was evaluated. Finally primer and probes were pre-spotted steadily on 96-well PCR plates and the practical applicability of the assay was proven with selected food products. The assay enables detection of multiple species of market relevance." @default.
- W2598423465 created "2017-04-07" @default.
- W2598423465 creator A5025944874 @default.
- W2598423465 creator A5032435095 @default.
- W2598423465 creator A5037650624 @default.
- W2598423465 creator A5065580457 @default.
- W2598423465 creator A5081981961 @default.
- W2598423465 creator A5084722833 @default.
- W2598423465 creator A5091845969 @default.
- W2598423465 date "2017-09-01" @default.
- W2598423465 modified "2023-10-04" @default.
- W2598423465 title "A novel screening approach based on six real-time PCR systems for the detection of crustacean species in food" @default.
- W2598423465 cites W1027068932 @default.
- W2598423465 cites W1975530730 @default.
- W2598423465 cites W1986568618 @default.
- W2598423465 cites W2024720124 @default.
- W2598423465 cites W2053100938 @default.
- W2598423465 cites W2062369625 @default.
- W2598423465 cites W2068545248 @default.
- W2598423465 cites W2073372618 @default.
- W2598423465 cites W2075594165 @default.
- W2598423465 cites W2163071289 @default.
- W2598423465 cites W2319668260 @default.
- W2598423465 cites W2396982136 @default.
- W2598423465 doi "https://doi.org/10.1016/j.foodcont.2017.03.019" @default.
- W2598423465 hasPublicationYear "2017" @default.
- W2598423465 type Work @default.
- W2598423465 sameAs 2598423465 @default.
- W2598423465 citedByCount "26" @default.
- W2598423465 countsByYear W25984234652018 @default.
- W2598423465 countsByYear W25984234652019 @default.
- W2598423465 countsByYear W25984234652020 @default.
- W2598423465 countsByYear W25984234652021 @default.
- W2598423465 countsByYear W25984234652022 @default.
- W2598423465 countsByYear W25984234652023 @default.
- W2598423465 crossrefType "journal-article" @default.
- W2598423465 hasAuthorship W2598423465A5025944874 @default.
- W2598423465 hasAuthorship W2598423465A5032435095 @default.
- W2598423465 hasAuthorship W2598423465A5037650624 @default.
- W2598423465 hasAuthorship W2598423465A5065580457 @default.
- W2598423465 hasAuthorship W2598423465A5081981961 @default.
- W2598423465 hasAuthorship W2598423465A5084722833 @default.
- W2598423465 hasAuthorship W2598423465A5091845969 @default.
- W2598423465 hasConcept C31903555 @default.
- W2598423465 hasConcept C505870484 @default.
- W2598423465 hasConcept C70721500 @default.
- W2598423465 hasConcept C84766238 @default.
- W2598423465 hasConcept C86803240 @default.
- W2598423465 hasConcept C90856448 @default.
- W2598423465 hasConceptScore W2598423465C31903555 @default.
- W2598423465 hasConceptScore W2598423465C505870484 @default.
- W2598423465 hasConceptScore W2598423465C70721500 @default.
- W2598423465 hasConceptScore W2598423465C84766238 @default.
- W2598423465 hasConceptScore W2598423465C86803240 @default.
- W2598423465 hasConceptScore W2598423465C90856448 @default.
- W2598423465 hasLocation W25984234651 @default.
- W2598423465 hasOpenAccess W2598423465 @default.
- W2598423465 hasPrimaryLocation W25984234651 @default.
- W2598423465 hasRelatedWork W1981434948 @default.
- W2598423465 hasRelatedWork W1989463223 @default.
- W2598423465 hasRelatedWork W2038437275 @default.
- W2598423465 hasRelatedWork W2044079403 @default.
- W2598423465 hasRelatedWork W2097001546 @default.
- W2598423465 hasRelatedWork W2115248090 @default.
- W2598423465 hasRelatedWork W2317912520 @default.
- W2598423465 hasRelatedWork W2935697799 @default.
- W2598423465 hasRelatedWork W2972137738 @default.
- W2598423465 hasRelatedWork W4250203818 @default.
- W2598423465 hasVolume "79" @default.
- W2598423465 isParatext "false" @default.
- W2598423465 isRetracted "false" @default.
- W2598423465 magId "2598423465" @default.
- W2598423465 workType "article" @default.