FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2599115106> ?p ?o ?g. }

• W2599115106 abstract "Transient receptor potential canonical 6 (TRPC6) channels are permeable to Na+ and Ca2+ and are widely expressed in the brain. In this study, the role of TRPC6 was investigated following ischemia/reperfusion (I/R) and oxygen-glucose deprivation (OGD). We found that TRPC6 expression was increased in wild-type (WT) mice cortical neurons following I/R and in primary neurons with OGD, and that deletion of TRPC6 reduced the I/R-induced brain infarct in mice and the OGD- /neurotoxin-induced neuronal death. Using live-cell imaging to examine intracellular Ca2+ levels ([Ca2+] i ), we found that OGD induced a significant higher increase in glutamate-evoked Ca2+ influx compared to untreated control and such an increase was reduced by TRPC6 deletion. Enhancement of TRPC6 expression using AdCMV-TRPC6-GFP infection in WT neurons increased [Ca2+] i in response to glutamate application compared to AdCMV-GFP control. Inhibition of N-methyl-d-aspartic acid receptor (NMDAR) with MK801 decreased TRPC6-dependent increase of [Ca2+] i in TRPC6 infected cells, indicating that such a Ca2+ influx was NMDAR dependent. Furthermore, TRPC6-dependent Ca2+ influx was blunted by blockade of Na+ entry in TRPC6 infected cells. Finally, OGD-enhanced Ca2+ influx was reduced, but not completely blocked, in the presence of voltage-dependent Na+ channel blocker tetrodotoxin (TTX) and dl-α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) blocker CNQX. Altogether, we concluded that I/R-induced brain damage was, in part, due to upregulation of TRPC6 in cortical neurons. We postulate that overexpression of TRPC6 following I/R may induce neuronal death partially through TRPC6-dependent Na+ entry which activated NMDAR, thus leading to a damaging Ca2+ overload. These findings may provide a potential target for future intervention in stroke-induced brain damage." @default.
• W2599115106 created "2017-04-07" @default.
• W2599115106 creator A5015199163 @default.
• W2599115106 creator A5019075682 @default.
• W2599115106 creator A5023969565 @default.
• W2599115106 creator A5037989560 @default.
• W2599115106 creator A5051705892 @default.
• W2599115106 creator A5068840254 @default.
• W2599115106 creator A5069869700 @default.
• W2599115106 creator A5075027403 @default.
• W2599115106 date "2017-03-28" @default.
• W2599115106 modified "2023-09-27" @default.
• W2599115106 title "Deletion of TRPC6 Attenuates NMDA Receptor-Mediated Ca2+ Entry and Ca2+-Induced Neurotoxicity Following Cerebral Ischemia and Oxygen-Glucose Deprivation" @default.
• W2599115106 cites W139878843 @default.
• W2599115106 cites W1456900775 @default.
• W2599115106 cites W1531356247 @default.
• W2599115106 cites W1647051175 @default.
• W2599115106 cites W1653897465 @default.
• W2599115106 cites W1657182769 @default.
• W2599115106 cites W1852612470 @default.
• W2599115106 cites W1905650613 @default.
• W2599115106 cites W1936450020 @default.
• W2599115106 cites W1963945020 @default.
• W2599115106 cites W1971442462 @default.
• W2599115106 cites W1972074180 @default.
• W2599115106 cites W1976379598 @default.
• W2599115106 cites W1976398898 @default.
• W2599115106 cites W1976696624 @default.
• W2599115106 cites W1978114446 @default.
• W2599115106 cites W1979406476 @default.
• W2599115106 cites W1981261137 @default.
• W2599115106 cites W1989173007 @default.
• W2599115106 cites W1992007780 @default.
• W2599115106 cites W1993536160 @default.
• W2599115106 cites W1995056605 @default.
• W2599115106 cites W1996381158 @default.
• W2599115106 cites W1997245611 @default.
• W2599115106 cites W2002401551 @default.
• W2599115106 cites W2003881958 @default.
• W2599115106 cites W2006376420 @default.
• W2599115106 cites W2007200514 @default.
• W2599115106 cites W2009278589 @default.
• W2599115106 cites W2009610734 @default.
• W2599115106 cites W2009611544 @default.
• W2599115106 cites W2016575029 @default.
• W2599115106 cites W2018043371 @default.
• W2599115106 cites W2018057744 @default.
• W2599115106 cites W2021456078 @default.
• W2599115106 cites W2022121838 @default.
• W2599115106 cites W2024923464 @default.
• W2599115106 cites W2025973200 @default.
• W2599115106 cites W2026874869 @default.
• W2599115106 cites W2027729490 @default.
• W2599115106 cites W2029949863 @default.
• W2599115106 cites W2031943544 @default.
• W2599115106 cites W2033737472 @default.
• W2599115106 cites W2034047115 @default.
• W2599115106 cites W2034579667 @default.
• W2599115106 cites W2036145275 @default.
• W2599115106 cites W2039483445 @default.
• W2599115106 cites W2044028200 @default.
• W2599115106 cites W2047315977 @default.
• W2599115106 cites W2049908093 @default.
• W2599115106 cites W2066581734 @default.
• W2599115106 cites W2075055448 @default.
• W2599115106 cites W2079637815 @default.
• W2599115106 cites W2081310545 @default.
• W2599115106 cites W2086302679 @default.
• W2599115106 cites W2087510546 @default.
• W2599115106 cites W2089011069 @default.
• W2599115106 cites W2089281537 @default.
• W2599115106 cites W2093421851 @default.
• W2599115106 cites W2094978516 @default.
• W2599115106 cites W2097293665 @default.
• W2599115106 cites W2100577158 @default.
• W2599115106 cites W2101716183 @default.
• W2599115106 cites W2105744792 @default.
• W2599115106 cites W2107123744 @default.
• W2599115106 cites W2112215250 @default.
• W2599115106 cites W2113608265 @default.
• W2599115106 cites W2115217673 @default.
• W2599115106 cites W2117266007 @default.
• W2599115106 cites W2123152434 @default.
• W2599115106 cites W2127012396 @default.
• W2599115106 cites W2137078662 @default.
• W2599115106 cites W2138227582 @default.
• W2599115106 cites W2139748951 @default.
• W2599115106 cites W2143782901 @default.
• W2599115106 cites W2146204489 @default.
• W2599115106 cites W2146266459 @default.
• W2599115106 cites W2149225235 @default.
• W2599115106 cites W2154499565 @default.
• W2599115106 cites W2161791427 @default.
• W2599115106 cites W2167280114 @default.
• W2599115106 cites W2301131921 @default.
• W2599115106 cites W2337167731 @default.
• W2599115106 cites W2399882212 @default.
• W2599115106 cites W2469523769 @default.
• W2599115106 cites W2485540697 @default.
• W2599115106 cites W2792583460 @default.

• next