Matches in SemOpenAlex for { <https://semopenalex.org/work/W2599702163> ?p ?o ?g. }
- W2599702163 abstract "A fundamental mechanism of the innate immune system is the recognition, via extra- and intracellular pattern-recognition receptors, of pathogen-associated molecular patterns. A prominent example is represented by foreign nucleic acids, triggering the activation of several signaling pathways. Among these, the endosomal toll-like receptor 7 (TLR7) is known to be activated by single-stranded RNA (ssRNA), which can be specifically influenced through elements of sequence structure and posttranscriptional modifications. Furthermore, small molecules TLR7 agonists (smTLRa) are applied as boosting adjuvants in vaccination processes. In this context, covalent conjugations between adjuvant and vaccines have been reported to exhibit synergistic effects. Here, we describe a concept to chemically combine three therapeutic functions in one RNA bioconjugate. This consists in the simultaneous TLR7 stimulation by ssRNA and smTLRa as well as the therapeutic function of the RNA itself, e.g., as a vaccinating or knockdown agent. We have hence synthesized bioconjugates of mRNA and siRNA containing covalently attached smTLRa and tested their function in TLR7 stimulation. Strikingly, the bioconjugates displayed decreased rather than synergistically increased stimulation. The decrease was distinct from the antagonistic action of an siRNA bearing a Gm motive, as observed by direct comparison of the effects in the presence of otherwise stimulatory RNA. In summary, these investigations showed that TRL7 activation can be impeded by bioconjugation of small molecules to RNA." @default.
- W2599702163 created "2017-04-07" @default.
- W2599702163 creator A5000883434 @default.
- W2599702163 creator A5001477363 @default.
- W2599702163 creator A5009074920 @default.
- W2599702163 creator A5013041850 @default.
- W2599702163 creator A5017323595 @default.
- W2599702163 creator A5019031942 @default.
- W2599702163 creator A5019358514 @default.
- W2599702163 creator A5027256234 @default.
- W2599702163 creator A5027412800 @default.
- W2599702163 creator A5032214407 @default.
- W2599702163 creator A5038034454 @default.
- W2599702163 creator A5042417952 @default.
- W2599702163 creator A5053412130 @default.
- W2599702163 creator A5068480727 @default.
- W2599702163 creator A5077129067 @default.
- W2599702163 creator A5091862305 @default.
- W2599702163 date "2017-03-24" @default.
- W2599702163 modified "2023-10-03" @default.
- W2599702163 title "Bioconjugation of Small Molecules to RNA Impedes Its Recognition by Toll-Like Receptor 7" @default.
- W2599702163 cites W1176162522 @default.
- W2599702163 cites W1521436470 @default.
- W2599702163 cites W1535643053 @default.
- W2599702163 cites W1542775986 @default.
- W2599702163 cites W1559117436 @default.
- W2599702163 cites W1590760212 @default.
- W2599702163 cites W1591392487 @default.
- W2599702163 cites W1596090014 @default.
- W2599702163 cites W1781506009 @default.
- W2599702163 cites W1919748804 @default.
- W2599702163 cites W1963706689 @default.
- W2599702163 cites W1965495205 @default.
- W2599702163 cites W1969072247 @default.
- W2599702163 cites W1972776583 @default.
- W2599702163 cites W1973659410 @default.
- W2599702163 cites W1986178107 @default.
- W2599702163 cites W1991236592 @default.
- W2599702163 cites W1994331999 @default.
- W2599702163 cites W1994391392 @default.
- W2599702163 cites W2005012808 @default.
- W2599702163 cites W2011559396 @default.
- W2599702163 cites W2011868039 @default.
- W2599702163 cites W2014992495 @default.
- W2599702163 cites W2017515700 @default.
- W2599702163 cites W2020368715 @default.
- W2599702163 cites W2024458873 @default.
- W2599702163 cites W2025980739 @default.
- W2599702163 cites W2028575270 @default.
- W2599702163 cites W2029827106 @default.
- W2599702163 cites W2034157885 @default.
- W2599702163 cites W2035148202 @default.
- W2599702163 cites W2042173485 @default.
- W2599702163 cites W2042288276 @default.
- W2599702163 cites W2042816890 @default.
- W2599702163 cites W2054205127 @default.
- W2599702163 cites W2054680036 @default.
- W2599702163 cites W2056210276 @default.
- W2599702163 cites W2062392673 @default.
- W2599702163 cites W2068012660 @default.
- W2599702163 cites W2070577667 @default.
- W2599702163 cites W2072118838 @default.
- W2599702163 cites W2077897130 @default.
- W2599702163 cites W2078890343 @default.
- W2599702163 cites W2093067468 @default.
- W2599702163 cites W2096917691 @default.
- W2599702163 cites W2098854209 @default.
- W2599702163 cites W2099610218 @default.
- W2599702163 cites W2100019645 @default.
- W2599702163 cites W2115855421 @default.
- W2599702163 cites W2116425120 @default.
- W2599702163 cites W2117229732 @default.
- W2599702163 cites W2118400571 @default.
- W2599702163 cites W2119293746 @default.
- W2599702163 cites W2123644491 @default.
- W2599702163 cites W2130312370 @default.
- W2599702163 cites W2136817553 @default.
- W2599702163 cites W2141137217 @default.
- W2599702163 cites W2143934902 @default.
- W2599702163 cites W2144733364 @default.
- W2599702163 cites W2146814159 @default.
- W2599702163 cites W2147763501 @default.
- W2599702163 cites W2150736048 @default.
- W2599702163 cites W2151777859 @default.
- W2599702163 cites W2152041138 @default.
- W2599702163 cites W2161208630 @default.
- W2599702163 cites W2165286214 @default.
- W2599702163 cites W2167438636 @default.
- W2599702163 cites W2195278272 @default.
- W2599702163 cites W2313435036 @default.
- W2599702163 cites W2343116876 @default.
- W2599702163 cites W2434872344 @default.
- W2599702163 cites W2472361557 @default.
- W2599702163 cites W2530273804 @default.
- W2599702163 cites W964387587 @default.
- W2599702163 doi "https://doi.org/10.3389/fimmu.2017.00312" @default.
- W2599702163 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5364167" @default.
- W2599702163 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28392787" @default.
- W2599702163 hasPublicationYear "2017" @default.
- W2599702163 type Work @default.