FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2599715321> ?p ?o ?g. }

• W2599715321 abstract "Experiments were conducted to develop and standardize the protocols forcryopreservation of sperm of common carp, Cyprinus carpio and also for using the cryopreserved sperm for fertilization of eggs. Nine extender solutions as Alsever's solution, kurokura-1, kurokura-2, urea egg-yolk, egg-yolk citrate, 0.6% glucose, 0.9% NaCl, Ma and Mb, and five cryoprotectants namely ethanol, methanol, dimethylsulfoxide (DMSO), dimethylamine (DMA) and glycerol were tested. The cryoprotectants were mixed at 10% concentration of the extenders (v/v) to make the cryodiluents. Milt andcryodiluents were mixed at a ratio of 1:9 for Alsever's solution, kurokura-1, kurokura-2, 0.6% glucose and 0.9% NaCl, 1:4 for urea egg-yolk, egg-yolk citrate, Ma and Mb. Among the cryodiluents Alsever's solution mixed with either ethanol or methanol was found to be suitable and it produced more than 90% and 80% spermatozoan motility at equilibrium and post-thaw periods, respectively. Kurokura-1 and kurokura-2 when mixed with the same cryoprotectants showed good spermatozoan motility at equilibriumperiod (80-90%) but the motility was reduced (30-55%) at post-thaw state. Other extenders did not produce acceptable sperm-motility and in some cases the frozen milt became clotted. Different dilution ratios (1:1, 1:2, 1:4, 1:5, 1:7, 1:9, 1:12, 1:15, 1:20) were formulated for obtaining a suitable milt dilution, the dilution ratio of 1: 9 (milt : cryodiluent) demonstrated the highest post-thaw spermatozoan motility (80%) in Alserver's solution. The optimum concentration of cryoprotectants in the cryodiluents was determined, 10% concentration level was found to be effective to produce the highest number of spermatozoan motility in comparison to the other concentrations (5%, 15%, 20% 30%). Sperm preserved with the cryodiluent Alsever's solution along with either methanol or ethanol was found to be effective to fertilize eggs and produce hatchlings. The hatching rates ranged between 1.48% and 14.76%, compare to control. The fish produced through use of cryopreserved sperm and normal sperm were found to grow well and no significant (P<0.05) growth difference was observed between them. In case of silver barb, Barbonymus gonionotus, sperm tested against six extenders such as egg-yolk citrate, urea-egg-yolk, kurokura-1, kurokura-2, 0.9% NaCl and modified fish ringer (MFR) solution. Cryoprotectants used were the same as those of C. carpio. Milt was diluted with the cryodiluent at a ratio of 1:4 for egg-yolk citrate and urea-egg-yolk, 1:5 for kurokura-1 and 1:9 for 0.9% NaCl, MFR and kurokura-2. The cryoprotectant concentration was maintained at 10% of the extender (v/v) in all the cases. Among the extenders, egg-yolk citrate and urea-egg-yolk mixed with 10% DMSO, methanol and ethanol produced 50% post-thaw spermatozoan motility, whereas DMA and glycerol provided only 10% motility. Trials on milt dilution ratio and cryoprotectant concentration are being conducted. Fertilization trials are also underway." @default.
• W2599715321 created "2017-04-07" @default.
• W2599715321 creator A5016717512 @default.
• W2599715321 creator A5030473694 @default.
• W2599715321 creator A5031278699 @default.
• W2599715321 creator A5036776938 @default.
• W2599715321 creator A5082919284 @default.
• W2599715321 date "2005-01-01" @default.
• W2599715321 modified "2023-09-23" @default.
• W2599715321 title "Cryopreservation of sperm of common carp, Cyprinus carpio and silver barb, Barbonymus gonionotus for genetically improved seed production" @default.
• W2599715321 hasPublicationYear "2005" @default.
• W2599715321 type Work @default.
• W2599715321 sameAs 2599715321 @default.
• W2599715321 citedByCount "0" @default.
• W2599715321 crossrefType "journal-article" @default.
• W2599715321 hasAuthorship W2599715321A5016717512 @default.
• W2599715321 hasAuthorship W2599715321A5030473694 @default.
• W2599715321 hasAuthorship W2599715321A5031278699 @default.
• W2599715321 hasAuthorship W2599715321A5036776938 @default.
• W2599715321 hasAuthorship W2599715321A5082919284 @default.
• W2599715321 hasConcept C105702510 @default.
• W2599715321 hasConcept C121332964 @default.
• W2599715321 hasConcept C126125632 @default.
• W2599715321 hasConcept C140793950 @default.
• W2599715321 hasConcept C178790620 @default.
• W2599715321 hasConcept C179933525 @default.
• W2599715321 hasConcept C185592680 @default.
• W2599715321 hasConcept C196843134 @default.
• W2599715321 hasConcept C2776586616 @default.
• W2599715321 hasConcept C2777632594 @default.
• W2599715321 hasConcept C2778093475 @default.
• W2599715321 hasConcept C2779578285 @default.
• W2599715321 hasConcept C2780326611 @default.
• W2599715321 hasConcept C2780365088 @default.
• W2599715321 hasConcept C2780881558 @default.
• W2599715321 hasConcept C2781087480 @default.
• W2599715321 hasConcept C2781321622 @default.
• W2599715321 hasConcept C2909208804 @default.
• W2599715321 hasConcept C31903555 @default.
• W2599715321 hasConcept C505870484 @default.
• W2599715321 hasConcept C55493867 @default.
• W2599715321 hasConcept C59822182 @default.
• W2599715321 hasConcept C68528507 @default.
• W2599715321 hasConcept C86803240 @default.
• W2599715321 hasConcept C88972607 @default.
• W2599715321 hasConcept C96618451 @default.
• W2599715321 hasConcept C97355855 @default.
• W2599715321 hasConceptScore W2599715321C105702510 @default.
• W2599715321 hasConceptScore W2599715321C121332964 @default.
• W2599715321 hasConceptScore W2599715321C126125632 @default.
• W2599715321 hasConceptScore W2599715321C140793950 @default.
• W2599715321 hasConceptScore W2599715321C178790620 @default.
• W2599715321 hasConceptScore W2599715321C179933525 @default.
• W2599715321 hasConceptScore W2599715321C185592680 @default.
• W2599715321 hasConceptScore W2599715321C196843134 @default.
• W2599715321 hasConceptScore W2599715321C2776586616 @default.
• W2599715321 hasConceptScore W2599715321C2777632594 @default.
• W2599715321 hasConceptScore W2599715321C2778093475 @default.
• W2599715321 hasConceptScore W2599715321C2779578285 @default.
• W2599715321 hasConceptScore W2599715321C2780326611 @default.
• W2599715321 hasConceptScore W2599715321C2780365088 @default.
• W2599715321 hasConceptScore W2599715321C2780881558 @default.
• W2599715321 hasConceptScore W2599715321C2781087480 @default.
• W2599715321 hasConceptScore W2599715321C2781321622 @default.
• W2599715321 hasConceptScore W2599715321C2909208804 @default.
• W2599715321 hasConceptScore W2599715321C31903555 @default.
• W2599715321 hasConceptScore W2599715321C505870484 @default.
• W2599715321 hasConceptScore W2599715321C55493867 @default.
• W2599715321 hasConceptScore W2599715321C59822182 @default.
• W2599715321 hasConceptScore W2599715321C68528507 @default.
• W2599715321 hasConceptScore W2599715321C86803240 @default.
• W2599715321 hasConceptScore W2599715321C88972607 @default.
• W2599715321 hasConceptScore W2599715321C96618451 @default.
• W2599715321 hasConceptScore W2599715321C97355855 @default.
• W2599715321 hasLocation W25997153211 @default.
• W2599715321 hasOpenAccess W2599715321 @default.
• W2599715321 hasPrimaryLocation W25997153211 @default.
• W2599715321 hasRelatedWork W1969584751 @default.
• W2599715321 hasRelatedWork W2012086532 @default.
• W2599715321 hasRelatedWork W2029431791 @default.
• W2599715321 hasRelatedWork W2031960526 @default.
• W2599715321 hasRelatedWork W2031966018 @default.
• W2599715321 hasRelatedWork W2034986099 @default.
• W2599715321 hasRelatedWork W2085447350 @default.
• W2599715321 hasRelatedWork W2105552477 @default.
• W2599715321 hasRelatedWork W2106088018 @default.
• W2599715321 hasRelatedWork W2107847718 @default.
• W2599715321 hasRelatedWork W2110970994 @default.
• W2599715321 hasRelatedWork W2155002701 @default.
• W2599715321 hasRelatedWork W2169216971 @default.
• W2599715321 hasRelatedWork W2263391388 @default.
• W2599715321 hasRelatedWork W2598630028 @default.
• W2599715321 hasRelatedWork W2600070462 @default.
• W2599715321 hasRelatedWork W2605605615 @default.
• W2599715321 hasRelatedWork W3041996736 @default.
• W2599715321 hasRelatedWork W3205055442 @default.
• W2599715321 hasRelatedWork W2184070577 @default.
• W2599715321 isParatext "false" @default.
• W2599715321 isRetracted "false" @default.
• W2599715321 magId "2599715321" @default.

• next