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• W2599957413 abstract "FISH is a useful method to identify individual chromosomes in a karyotype and to discover their structural changes accompanying genome evolution and speciation. DNA probes for FISH should be chromosome specific and/or exhibit specific patterns of distribution along each chromosome. Such probes are not available in many plants including meadow fescue (<i>Festuca pratensis</i> Huds.), an important forage grass species. In the present study, various DNA repeats identified in Illumina shotgun sequences specific to chromosome 4F of <i>F. pratensis</i> were used as probes for FISH to develop the molecular karyotype of meadow fescue and to reveal a long-range molecular organization of its chromosomes. Five tandem repeats produced specific patterns on individual chromosomes. Their use in combination with probes for rRNA genes enabled the establishment of the molecular karyotype of meadow fescue. Most of the mobile genetic elements were dispersed along all the chromosomes except for the DNA transposon CACTA, which was localized preferentially to telomeric and subtelomeric regions, and a putative LTR element, which was localized to (peri)centromeric regions. Cytogenetic mapping of the 5 tandem repeats in other accessions of meadow fescue showed a highly similar distribution and confirmed the versatility and robustness of these probes." @default.
• W2599957413 created "2017-04-07" @default.
• W2599957413 creator A5011548029 @default.
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• W2599957413 creator A5063337128 @default.
• W2599957413 date "2017-01-01" @default.
• W2599957413 modified "2023-10-16" @default.
• W2599957413 title "Repetitive DNA: A Versatile Tool for Karyotyping in<b><i> Festuca pratensis</i></b> Huds." @default.
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• W2599957413 doi "https://doi.org/10.1159/000462915" @default.
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• W2599957413 hasPublicationYear "2017" @default.
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