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• W2599996451 abstract "Received: Revised: Accepted: Online available: June 20, 2015 September 14, 2015 September 27, 2015 January 09, 2016 Diarrheagenic Escherichia coli (DEC) are considered one of the major etiological agents of neonatal calf diarrhea (NCD). One hundred calves (2-21 days-old) suffering from diarrhea in Sharkia Governorate, Egypt were used in this study. Six E. coli virulence genes were characterized by multiplex PCR including; heat-stable enterotoxins (STa; 33.3%), heat-labile enterotoxin (LT; 30%), two Shiga toxins: (Stx1; 86.67%), (Stx2; 26.67%) produced from Shiga toxin producing E. coli (STEC) and two enterotoxigenic E. coli (ETEC) adhesions (F5; 13.3%) and (F41; 16.67%). Each isolate was found to carry one or more virulence genes. Clinicobiochemical examination showed significant (P<0.01) increase in serum total protein, globulin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine, blood urea nitrogen (BUN), and potassium levels. Meanwhile; serum albumin, A/G ratio, sodium and chloride levels were significantly (P<0.01) decreased. Multiplex PCR was a useful quick diagnostic tool for characterization and identification of E. coli virulence genes. Shiga toxin genes increase the risk for zoonotic STEC infection. Mixed E. coli infection is strongly associated with the reported cases of diarrhea due to the presence of several virulence genes in some isolates." @default.
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• W2599996451 date "2016-01-01" @default.
• W2599996451 modified "2023-09-28" @default.
• W2599996451 title "Epidemiological Study of Diarrheagenic Escherichia coli Virulence Genes in Newborn Calves" @default.
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