Matches in SemOpenAlex for { <https://semopenalex.org/work/W2600111101> ?p ?o ?g. }
- W2600111101 endingPage "410" @default.
- W2600111101 startingPage "392" @default.
- W2600111101 abstract "CD4 T cells are important cellular targets for HIV-1, yet the primary site of HIV fusion remains unresolved. Candidate fusion sites are either the plasma membrane or from within endosomes. One area of investigation compounding the controversy of this field, is the role of the protein dynamin in the HIV life cycle. To understand the role of dynamin in primary CD4 T cells we combined dynamin inhibition with a series of complementary assays based on single particle tracking, HIV fusion, detection of HIV DNA products and active viral transcription. We identify 3 levels of dynamin influence on the HIV life cycle. Firstly, dynamin influences productive infection by preventing cell cycle progression. Secondly, dynamin influences endocytosis rates and increases the probability of endosomal fusion. Finally, we provide evidence in resting CD4 T cells that dynamin directly regulates the HIV fusion reaction at the plasma membrane. We confirm this latter observation using 2 divergent dynamin modulating compounds, one that enhances dynamin conformations associated with dynamin ring formation (ryngo-1-23) and the other that preferentially targets dynamin conformations that appear in helices (dyngo-4a). This in-depth understanding of dynamin's roles in HIV infection clarifies recent controversies and furthermore provides evidence for dynamin regulation specifically in the HIV fusion reaction." @default.
- W2600111101 created "2017-04-07" @default.
- W2600111101 creator A5005523254 @default.
- W2600111101 creator A5023524835 @default.
- W2600111101 creator A5026633618 @default.
- W2600111101 creator A5026874815 @default.
- W2600111101 creator A5046451927 @default.
- W2600111101 creator A5054938965 @default.
- W2600111101 creator A5055970391 @default.
- W2600111101 creator A5065785507 @default.
- W2600111101 creator A5072227420 @default.
- W2600111101 date "2017-04-11" @default.
- W2600111101 modified "2023-09-26" @default.
- W2600111101 title "HIV infection is influenced by dynamin at 3 independent points in the viral life cycle" @default.
- W2600111101 cites W108460990 @default.
- W2600111101 cites W1484670943 @default.
- W2600111101 cites W1505771931 @default.
- W2600111101 cites W1526128150 @default.
- W2600111101 cites W1588423409 @default.
- W2600111101 cites W1589762717 @default.
- W2600111101 cites W1802232529 @default.
- W2600111101 cites W1881736060 @default.
- W2600111101 cites W1889215297 @default.
- W2600111101 cites W1963569988 @default.
- W2600111101 cites W1965019797 @default.
- W2600111101 cites W1972623359 @default.
- W2600111101 cites W1981440980 @default.
- W2600111101 cites W1981644527 @default.
- W2600111101 cites W1986290362 @default.
- W2600111101 cites W1989035987 @default.
- W2600111101 cites W1990522629 @default.
- W2600111101 cites W1994225467 @default.
- W2600111101 cites W1996183144 @default.
- W2600111101 cites W1996435746 @default.
- W2600111101 cites W1996865233 @default.
- W2600111101 cites W1998750685 @default.
- W2600111101 cites W2001402908 @default.
- W2600111101 cites W2005394493 @default.
- W2600111101 cites W2013327420 @default.
- W2600111101 cites W2016967864 @default.
- W2600111101 cites W2022199868 @default.
- W2600111101 cites W2025698648 @default.
- W2600111101 cites W2026939674 @default.
- W2600111101 cites W2027208002 @default.
- W2600111101 cites W2029379203 @default.
- W2600111101 cites W2030039293 @default.
- W2600111101 cites W2033955212 @default.
- W2600111101 cites W2035655655 @default.
- W2600111101 cites W2037404071 @default.
- W2600111101 cites W2039028257 @default.
- W2600111101 cites W2041875417 @default.
- W2600111101 cites W2041982728 @default.
- W2600111101 cites W2043287597 @default.
- W2600111101 cites W2045626973 @default.
- W2600111101 cites W2047900444 @default.
- W2600111101 cites W2053527054 @default.
- W2600111101 cites W2054876749 @default.
- W2600111101 cites W2059561803 @default.
- W2600111101 cites W2060139150 @default.
- W2600111101 cites W2061143375 @default.
- W2600111101 cites W2067311108 @default.
- W2600111101 cites W2076894542 @default.
- W2600111101 cites W2080702547 @default.
- W2600111101 cites W2081489255 @default.
- W2600111101 cites W2081602743 @default.
- W2600111101 cites W2084803259 @default.
- W2600111101 cites W2089676893 @default.
- W2600111101 cites W2097494814 @default.
- W2600111101 cites W2100265029 @default.
- W2600111101 cites W2105384628 @default.
- W2600111101 cites W2106296961 @default.
- W2600111101 cites W2116618689 @default.
- W2600111101 cites W2117891939 @default.
- W2600111101 cites W2118545611 @default.
- W2600111101 cites W2119490035 @default.
- W2600111101 cites W2120071061 @default.
- W2600111101 cites W2120589083 @default.
- W2600111101 cites W2131376910 @default.
- W2600111101 cites W2133503825 @default.
- W2600111101 cites W2136595144 @default.
- W2600111101 cites W2142494698 @default.
- W2600111101 cites W2142655367 @default.
- W2600111101 cites W2143459780 @default.
- W2600111101 cites W2146706111 @default.
- W2600111101 cites W2147469910 @default.
- W2600111101 cites W2151111558 @default.
- W2600111101 cites W2152204052 @default.
- W2600111101 cites W2154156417 @default.
- W2600111101 cites W2154976728 @default.
- W2600111101 cites W2157852305 @default.
- W2600111101 cites W2157920366 @default.
- W2600111101 cites W2159120707 @default.
- W2600111101 cites W2163059437 @default.
- W2600111101 cites W2171252684 @default.
- W2600111101 cites W2569395967 @default.
- W2600111101 cites W4235560636 @default.
- W2600111101 cites W4237038146 @default.
- W2600111101 cites W4249806757 @default.