Matches in SemOpenAlex for { <https://semopenalex.org/work/W2600244711> ?p ?o ?g. }
- W2600244711 abstract "Strict L-chiral rejection through Gly-cisPro motif during chiral proofreading underlies the inability of D-aminoacyl-tRNA deacylase (DTD) to discriminate between D-amino acids and achiral glycine. The consequent Gly-tRNAGly ‘misediting paradox’ is resolved by EF-Tu in the cell. Here, we show that DTD’s active site architecture can efficiently edit mischarged Gly-tRNAAla species four orders of magnitude more efficiently than even AlaRS, the only ubiquitous cellular checkpoint known for clearing the error. Also, DTD knockout in AlaRS editing-defective background causes pronounced toxicity in Escherichia coli even at low-glycine levels which is alleviated by alanine supplementation. We further demonstrate that DTD positively selects the universally invariant tRNAAla-specific G3•U70. Moreover, DTD’s activity on non-cognate Gly-tRNAAla is conserved across all bacteria and eukaryotes, suggesting DTD’s key cellular role as a glycine deacylator. Our study thus reveals a hitherto unknown function of DTD in cracking the universal mechanistic dilemma encountered by AlaRS, and its physiological importance." @default.
- W2600244711 created "2017-04-07" @default.
- W2600244711 creator A5005313006 @default.
- W2600244711 creator A5007673471 @default.
- W2600244711 creator A5021687539 @default.
- W2600244711 creator A5040415111 @default.
- W2600244711 creator A5041170482 @default.
- W2600244711 creator A5059969346 @default.
- W2600244711 creator A5074061512 @default.
- W2600244711 date "2017-03-31" @default.
- W2600244711 modified "2023-10-17" @default.
- W2600244711 title "Role of D-aminoacyl-tRNA deacylase beyond chiral proofreading as a cellular defense against glycine mischarging by AlaRS" @default.
- W2600244711 cites W1965897796 @default.
- W2600244711 cites W1966840835 @default.
- W2600244711 cites W1967448903 @default.
- W2600244711 cites W1970563053 @default.
- W2600244711 cites W1971604056 @default.
- W2600244711 cites W1973010540 @default.
- W2600244711 cites W1973882091 @default.
- W2600244711 cites W1978453601 @default.
- W2600244711 cites W1980382519 @default.
- W2600244711 cites W1985888036 @default.
- W2600244711 cites W1993111592 @default.
- W2600244711 cites W2003466068 @default.
- W2600244711 cites W2004607722 @default.
- W2600244711 cites W2008676983 @default.
- W2600244711 cites W2011699532 @default.
- W2600244711 cites W2013165826 @default.
- W2600244711 cites W2013311090 @default.
- W2600244711 cites W2019785852 @default.
- W2600244711 cites W2022592785 @default.
- W2600244711 cites W2025666847 @default.
- W2600244711 cites W2026836226 @default.
- W2600244711 cites W2027340117 @default.
- W2600244711 cites W2033843196 @default.
- W2600244711 cites W2037242504 @default.
- W2600244711 cites W2046151253 @default.
- W2600244711 cites W2047792576 @default.
- W2600244711 cites W2049504572 @default.
- W2600244711 cites W2057781025 @default.
- W2600244711 cites W2058159886 @default.
- W2600244711 cites W2061659241 @default.
- W2600244711 cites W2064580738 @default.
- W2600244711 cites W2066392481 @default.
- W2600244711 cites W2071935868 @default.
- W2600244711 cites W2073484200 @default.
- W2600244711 cites W2081351825 @default.
- W2600244711 cites W2088815894 @default.
- W2600244711 cites W2089328521 @default.
- W2600244711 cites W2090783184 @default.
- W2600244711 cites W2091594333 @default.
- W2600244711 cites W2101829922 @default.
- W2600244711 cites W2108465110 @default.
- W2600244711 cites W2111967267 @default.
- W2600244711 cites W2115094449 @default.
- W2600244711 cites W2116137883 @default.
- W2600244711 cites W2117430673 @default.
- W2600244711 cites W2120744038 @default.
- W2600244711 cites W2125768570 @default.
- W2600244711 cites W2132276503 @default.
- W2600244711 cites W2137532652 @default.
- W2600244711 cites W2140020971 @default.
- W2600244711 cites W2140885472 @default.
- W2600244711 cites W2156130516 @default.
- W2600244711 cites W2163209588 @default.
- W2600244711 cites W2168383728 @default.
- W2600244711 cites W2256383763 @default.
- W2600244711 cites W2402723633 @default.
- W2600244711 cites W2407907541 @default.
- W2600244711 cites W2583124926 @default.
- W2600244711 doi "https://doi.org/10.7554/elife.24001" @default.
- W2600244711 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5409826" @default.
- W2600244711 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28362257" @default.
- W2600244711 hasPublicationYear "2017" @default.
- W2600244711 type Work @default.
- W2600244711 sameAs 2600244711 @default.
- W2600244711 citedByCount "27" @default.
- W2600244711 countsByYear W26002447112017 @default.
- W2600244711 countsByYear W26002447112018 @default.
- W2600244711 countsByYear W26002447112019 @default.
- W2600244711 countsByYear W26002447112020 @default.
- W2600244711 countsByYear W26002447112022 @default.
- W2600244711 countsByYear W26002447112023 @default.
- W2600244711 crossrefType "journal-article" @default.
- W2600244711 hasAuthorship W2600244711A5005313006 @default.
- W2600244711 hasAuthorship W2600244711A5007673471 @default.
- W2600244711 hasAuthorship W2600244711A5021687539 @default.
- W2600244711 hasAuthorship W2600244711A5040415111 @default.
- W2600244711 hasAuthorship W2600244711A5041170482 @default.
- W2600244711 hasAuthorship W2600244711A5059969346 @default.
- W2600244711 hasAuthorship W2600244711A5074061512 @default.
- W2600244711 hasBestOaLocation W26002447111 @default.
- W2600244711 hasConcept C104317684 @default.
- W2600244711 hasConcept C153957851 @default.
- W2600244711 hasConcept C170748874 @default.
- W2600244711 hasConcept C2777756961 @default.
- W2600244711 hasConcept C2779856020 @default.
- W2600244711 hasConcept C515207424 @default.
- W2600244711 hasConcept C54355233 @default.
- W2600244711 hasConcept C552990157 @default.