Matches in SemOpenAlex for { <https://semopenalex.org/work/W2600248822> ?p ?o ?g. }
- W2600248822 abstract "Abstract Quantitative analysis of fluorescence signals from cells reacted with fluorescently labeled probes is a widely-used method for assessing cell biology. This method has become especially powerful for screening novel nanostructured materials for their influence on cell behavior. However, the effect of nanostructured surface on fluorescence intensity has largely been ignored, which likely leads to erroneous conclusions about cell behavior. This paper investigates this possibility by using fibroblasts cultured on nanoporous gold (np-Au) as a model nanostructured material system. We found that fibroblasts stained for f-actin using phalloidin conjugated with common fluorophores display different levels of fluorescence on np-Au, planar gold, and glass, suggesting different levels of f-actin composition. However, direct quantification via western blots indicates that the actin expression is the same across all conditions. We further investigated whether the fluorescence intensity depended on np-Au feature size, complementing the findings with reflection dark field measurements from different np-Au surfaces. Overall, our experimental measurements in agreement with our electrodynamic simulations suggest that nanostructured surfaces alter the fluorescence intensity of fluorophores by modulating both the excitation and light emission processes. We conclude that comparison of fluorescence on materials with different nanostructures should be done with a quantification method decoupled from the nanostructure's influence." @default.
- W2600248822 created "2017-04-07" @default.
- W2600248822 creator A5019478535 @default.
- W2600248822 creator A5020257734 @default.
- W2600248822 creator A5020564110 @default.
- W2600248822 creator A5022499603 @default.
- W2600248822 creator A5066444182 @default.
- W2600248822 creator A5074323488 @default.
- W2600248822 date "2017-03-27" @default.
- W2600248822 modified "2023-09-27" @default.
- W2600248822 title "Nanostructure Introduces Artifacts in Quantitative Immunofluorescence by Influencing Fluorophore Intensity" @default.
- W2600248822 cites W1800553252 @default.
- W2600248822 cites W1883449737 @default.
- W2600248822 cites W1971528407 @default.
- W2600248822 cites W1972081276 @default.
- W2600248822 cites W1981131401 @default.
- W2600248822 cites W1989948313 @default.
- W2600248822 cites W2003755130 @default.
- W2600248822 cites W2003978286 @default.
- W2600248822 cites W2019133050 @default.
- W2600248822 cites W2024240793 @default.
- W2600248822 cites W2044922077 @default.
- W2600248822 cites W2046075862 @default.
- W2600248822 cites W2061995191 @default.
- W2600248822 cites W2072982772 @default.
- W2600248822 cites W2083926174 @default.
- W2600248822 cites W2086996612 @default.
- W2600248822 cites W2091766680 @default.
- W2600248822 cites W2103215282 @default.
- W2600248822 cites W2106221863 @default.
- W2600248822 cites W2112165279 @default.
- W2600248822 cites W2116438704 @default.
- W2600248822 cites W2121830147 @default.
- W2600248822 cites W2123034204 @default.
- W2600248822 cites W2124733101 @default.
- W2600248822 cites W2129191745 @default.
- W2600248822 cites W2138238561 @default.
- W2600248822 cites W2141201649 @default.
- W2600248822 cites W2148924929 @default.
- W2600248822 cites W2152004144 @default.
- W2600248822 cites W2158990131 @default.
- W2600248822 cites W2182415026 @default.
- W2600248822 cites W2322981765 @default.
- W2600248822 cites W2323419362 @default.
- W2600248822 cites W2398660212 @default.
- W2600248822 cites W2419379961 @default.
- W2600248822 cites W978879985 @default.
- W2600248822 doi "https://doi.org/10.1038/s41598-017-00447-7" @default.
- W2600248822 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5428417" @default.
- W2600248822 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28348397" @default.
- W2600248822 hasPublicationYear "2017" @default.
- W2600248822 type Work @default.
- W2600248822 sameAs 2600248822 @default.
- W2600248822 citedByCount "4" @default.
- W2600248822 countsByYear W26002488222017 @default.
- W2600248822 countsByYear W26002488222020 @default.
- W2600248822 countsByYear W26002488222021 @default.
- W2600248822 crossrefType "journal-article" @default.
- W2600248822 hasAuthorship W2600248822A5019478535 @default.
- W2600248822 hasAuthorship W2600248822A5020257734 @default.
- W2600248822 hasAuthorship W2600248822A5020564110 @default.
- W2600248822 hasAuthorship W2600248822A5022499603 @default.
- W2600248822 hasAuthorship W2600248822A5066444182 @default.
- W2600248822 hasAuthorship W2600248822A5074323488 @default.
- W2600248822 hasBestOaLocation W26002488221 @default.
- W2600248822 hasConcept C120665830 @default.
- W2600248822 hasConcept C121332964 @default.
- W2600248822 hasConcept C12554922 @default.
- W2600248822 hasConcept C171250308 @default.
- W2600248822 hasConcept C186187911 @default.
- W2600248822 hasConcept C192562407 @default.
- W2600248822 hasConcept C2778617687 @default.
- W2600248822 hasConcept C48940184 @default.
- W2600248822 hasConcept C86803240 @default.
- W2600248822 hasConcept C91881484 @default.
- W2600248822 hasConceptScore W2600248822C120665830 @default.
- W2600248822 hasConceptScore W2600248822C121332964 @default.
- W2600248822 hasConceptScore W2600248822C12554922 @default.
- W2600248822 hasConceptScore W2600248822C171250308 @default.
- W2600248822 hasConceptScore W2600248822C186187911 @default.
- W2600248822 hasConceptScore W2600248822C192562407 @default.
- W2600248822 hasConceptScore W2600248822C2778617687 @default.
- W2600248822 hasConceptScore W2600248822C48940184 @default.
- W2600248822 hasConceptScore W2600248822C86803240 @default.
- W2600248822 hasConceptScore W2600248822C91881484 @default.
- W2600248822 hasIssue "1" @default.
- W2600248822 hasLocation W26002488221 @default.
- W2600248822 hasLocation W26002488222 @default.
- W2600248822 hasLocation W26002488223 @default.
- W2600248822 hasLocation W26002488224 @default.
- W2600248822 hasLocation W26002488225 @default.
- W2600248822 hasLocation W26002488226 @default.
- W2600248822 hasLocation W26002488227 @default.
- W2600248822 hasLocation W26002488228 @default.
- W2600248822 hasOpenAccess W2600248822 @default.
- W2600248822 hasPrimaryLocation W26002488221 @default.
- W2600248822 hasRelatedWork W2021078741 @default.
- W2600248822 hasRelatedWork W2059851901 @default.
- W2600248822 hasRelatedWork W2073064246 @default.
- W2600248822 hasRelatedWork W2192350152 @default.