Matches in SemOpenAlex for { <https://semopenalex.org/work/W2600422497> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2600422497 abstract "e15577 Background: Only specific subpopulations of tumor cells, so called cancer stem cells (CSC) may initiate and maintain tumors. The phenotype and molecular properties of ovarian CSC remain elusive. Aldehyde dehydrogenase (ALDH) activity characterizes (cancer) stem cells in different tissues and has been associated with ovarian CSC (Silva et al, 2011; Kryczek et al, 2012). Contradictory results have been reported on ALDH1 expression and prognosis in ovarian carcinoma. In this study, we explore the role of ALDH in serous ovarian carcinoma (SOC). Methods: Aldefluor-staining was used to assess ALDH activity in different ovarian carcinoma cell-lines and patient samples. ALDH+ and ALDH- cells isolated by FACS and ALDH1 versus control siRNA treated cells were analyzed in sphere forming, proliferation, BrdU and cell cycle assays. In vivo tumorigenicity assays including serial re-transplantations were performed in NOD/SCID/IL2Rγ null mice. ALDH1 and Ki67 expression were assessed immunohistochemically on a tissue microarray of 152 SOC samples. Results: ALDH+ cells formed more tumor spheres than ALDH- cells from the OVCAR-3 cell line and primary SOC and larger spheres (> 5.000 µm²) developed solely from ALDH+ cells. However, in vivo both cell fractions gave rise to tumors. Tumors contained both ALDH+ and ALDH- cells irrespective of the starting population. Notably, ALDH+ cells generated tumors more rapidly and induced larger tumors, suggesting a higher proliferative capacity. Immunohistochemical analysis of a larger cohort of SOC patients confirmed association of ALDH1 expression with the proliferation marker Ki67 (p=0.007). Surprisingly, co-stainings revealed that ALDH1 positive cells were mostly Ki67 negative and cell cycle synchronisation experiments using different agents showed ALDH induction in G0-enriched OVCAR-3 cells. However, inhibition of ALDH by treatment with three distinct siRNAs against ALDH1 did not alter cell cycle distribution. Conclusions: Our data suggest that ALDH is a correlative marker indicating, but not actively sustaining a quiescent stem-cell like state in SOC. Upon exit from G0, ALDH+ cells lose ALDH expression and induce a proliferative response." @default.
- W2600422497 created "2017-04-07" @default.
- W2600422497 creator A5008104862 @default.
- W2600422497 creator A5011848384 @default.
- W2600422497 creator A5014642548 @default.
- W2600422497 creator A5027983567 @default.
- W2600422497 creator A5030552991 @default.
- W2600422497 creator A5032246802 @default.
- W2600422497 creator A5056169995 @default.
- W2600422497 creator A5066624700 @default.
- W2600422497 creator A5067827022 @default.
- W2600422497 creator A5076745558 @default.
- W2600422497 creator A5086791040 @default.
- W2600422497 creator A5088208446 @default.
- W2600422497 date "2012-05-20" @default.
- W2600422497 modified "2023-09-27" @default.
- W2600422497 title "Aldehyde dehydrogenase activity in serous ovarian carcinoma." @default.
- W2600422497 doi "https://doi.org/10.1200/jco.2012.30.15_suppl.e15577" @default.
- W2600422497 hasPublicationYear "2012" @default.
- W2600422497 type Work @default.
- W2600422497 sameAs 2600422497 @default.
- W2600422497 citedByCount "0" @default.
- W2600422497 crossrefType "journal-article" @default.
- W2600422497 hasAuthorship W2600422497A5008104862 @default.
- W2600422497 hasAuthorship W2600422497A5011848384 @default.
- W2600422497 hasAuthorship W2600422497A5014642548 @default.
- W2600422497 hasAuthorship W2600422497A5027983567 @default.
- W2600422497 hasAuthorship W2600422497A5030552991 @default.
- W2600422497 hasAuthorship W2600422497A5032246802 @default.
- W2600422497 hasAuthorship W2600422497A5056169995 @default.
- W2600422497 hasAuthorship W2600422497A5066624700 @default.
- W2600422497 hasAuthorship W2600422497A5067827022 @default.
- W2600422497 hasAuthorship W2600422497A5076745558 @default.
- W2600422497 hasAuthorship W2600422497A5086791040 @default.
- W2600422497 hasAuthorship W2600422497A5088208446 @default.
- W2600422497 hasConcept C121608353 @default.
- W2600422497 hasConcept C126322002 @default.
- W2600422497 hasConcept C143998085 @default.
- W2600422497 hasConcept C150173356 @default.
- W2600422497 hasConcept C181199279 @default.
- W2600422497 hasConcept C25747580 @default.
- W2600422497 hasConcept C2777546739 @default.
- W2600422497 hasConcept C2777632260 @default.
- W2600422497 hasConcept C2780427987 @default.
- W2600422497 hasConcept C29456083 @default.
- W2600422497 hasConcept C3019054536 @default.
- W2600422497 hasConcept C502942594 @default.
- W2600422497 hasConcept C55493867 @default.
- W2600422497 hasConcept C71924100 @default.
- W2600422497 hasConcept C86803240 @default.
- W2600422497 hasConceptScore W2600422497C121608353 @default.
- W2600422497 hasConceptScore W2600422497C126322002 @default.
- W2600422497 hasConceptScore W2600422497C143998085 @default.
- W2600422497 hasConceptScore W2600422497C150173356 @default.
- W2600422497 hasConceptScore W2600422497C181199279 @default.
- W2600422497 hasConceptScore W2600422497C25747580 @default.
- W2600422497 hasConceptScore W2600422497C2777546739 @default.
- W2600422497 hasConceptScore W2600422497C2777632260 @default.
- W2600422497 hasConceptScore W2600422497C2780427987 @default.
- W2600422497 hasConceptScore W2600422497C29456083 @default.
- W2600422497 hasConceptScore W2600422497C3019054536 @default.
- W2600422497 hasConceptScore W2600422497C502942594 @default.
- W2600422497 hasConceptScore W2600422497C55493867 @default.
- W2600422497 hasConceptScore W2600422497C71924100 @default.
- W2600422497 hasConceptScore W2600422497C86803240 @default.
- W2600422497 hasLocation W26004224971 @default.
- W2600422497 hasOpenAccess W2600422497 @default.
- W2600422497 hasPrimaryLocation W26004224971 @default.
- W2600422497 hasRelatedWork W1978695677 @default.
- W2600422497 hasRelatedWork W2007195392 @default.
- W2600422497 hasRelatedWork W2055540745 @default.
- W2600422497 hasRelatedWork W2065560906 @default.
- W2600422497 hasRelatedWork W2065636563 @default.
- W2600422497 hasRelatedWork W2069845949 @default.
- W2600422497 hasRelatedWork W2074982201 @default.
- W2600422497 hasRelatedWork W2138784667 @default.
- W2600422497 hasRelatedWork W2150963187 @default.
- W2600422497 hasRelatedWork W2151879335 @default.
- W2600422497 hasRelatedWork W2315159229 @default.
- W2600422497 hasRelatedWork W2336436999 @default.
- W2600422497 hasRelatedWork W2511533585 @default.
- W2600422497 hasRelatedWork W2589517913 @default.
- W2600422497 hasRelatedWork W2593896130 @default.
- W2600422497 hasRelatedWork W2776158501 @default.
- W2600422497 hasRelatedWork W281712575 @default.
- W2600422497 hasRelatedWork W2885487975 @default.
- W2600422497 hasRelatedWork W2947484205 @default.
- W2600422497 hasRelatedWork W3083638063 @default.
- W2600422497 isParatext "false" @default.
- W2600422497 isRetracted "false" @default.
- W2600422497 magId "2600422497" @default.
- W2600422497 workType "article" @default.