FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2600598352> ?p ?o ?g. }

• W2600598352 endingPage "106" @default.
• W2600598352 startingPage "95" @default.
• W2600598352 abstract "Nucleotides stimulate phosphorylation of CREB to induce cell proliferation and survival in diverse cell types. We report here that ADP induces the phosphorylation of CREB in a time- and concentration-dependent manner in chick embryo retinal progenitors in culture. ADP-induced increase in phospho-CREB is mediated by P2 receptors as it is blocked by PPADS but not by the adenosine antagonists DPCPX or ZM241385. Incubation of the cultures with the CREB inhibitor KG-501 prevents ADP-induced incorporation of [3H]-thymidine, indicating that CREB is involved in retinal cell proliferation. No effect of this compound is observed on the viability of retinal progenitors. While no significant increase in CREB phosphorylation is observed with the P2Y1 receptor agonist MRS2365, ADP-induced phosphorylation of CREB is blocked by the P2Y13 receptor selective antagonist MRS2211, but not by MRS2179 or PSB0739, two antagonists of the P2Y1 and P2Y12 receptors, respectively, suggesting that ADP-induced CREB phosphorylation is mediated by P2Y13 receptors. ADP-induced increase in phospho-CREB is attenuated by the PI3K inhibitor LY294002 and completely prevented by the MEK inhibitor U0126, suggesting that at least ERK is involved in ADP-induced CREB phosphorylation. A pharmacological profile similar to the activation and inhibition of CREB phosphorylation is observed in the phosphorylation of ERK, suggesting that P2Y13 receptors mediate ADP induced ERK/CREB pathway in the cultures. While no increase in [3H]-thymidine incorporation is observed with the P2Y1 receptor agonist MRS2365, both MRS2179 and MRS2211 prevent ADP-mediated increase in [3H]-thymidine incorporation, but not progenitor's survival, suggesting that both P2Y1 and P2Y13 receptor subtypes are involved in ADP-induced cell proliferation. P2Y1 receptor-mediated increase in [Ca2+]i is observed in glial cells only when cultures maintained for 9days are used. In glia from cultures cultivated for only 2days, no increase in [Ca2+]i is detected with MRS2365 and no inhibition of ADP-mediated calcium response is observed with MRS2179. In contrast, MRS2211 attenuates ADP-mediated increase in [Ca2+]i in glial cells from cultures at both stages, suggesting the presence of P2Y13 receptors coupled to calcium mobilization in proliferating retinal glial progenitors in culture." @default.
• W2600598352 created "2017-04-07" @default.
• W2600598352 creator A5002353034 @default.
• W2600598352 creator A5048416585 @default.
• W2600598352 creator A5050267628 @default.
• W2600598352 creator A5066212399 @default.
• W2600598352 creator A5082054461 @default.
• W2600598352 date "2017-07-01" @default.
• W2600598352 modified "2023-10-17" @default.
• W2600598352 title "Nucleotide P2Y13-stimulated phosphorylation of CREB is required for ADP-induced proliferation of late developing retinal glial progenitors in culture" @default.
• W2600598352 cites W1507007180 @default.
• W2600598352 cites W1515824334 @default.
• W2600598352 cites W1836457398 @default.
• W2600598352 cites W1968656579 @default.
• W2600598352 cites W1970277597 @default.
• W2600598352 cites W1972893985 @default.
• W2600598352 cites W1976759540 @default.
• W2600598352 cites W1980026136 @default.
• W2600598352 cites W1981536211 @default.
• W2600598352 cites W1983474000 @default.
• W2600598352 cites W1984832762 @default.
• W2600598352 cites W1988231418 @default.
• W2600598352 cites W1991113140 @default.
• W2600598352 cites W1991693530 @default.
• W2600598352 cites W1991928587 @default.
• W2600598352 cites W1993379513 @default.
• W2600598352 cites W1994038425 @default.
• W2600598352 cites W1998100172 @default.
• W2600598352 cites W1998523437 @default.
• W2600598352 cites W1998626937 @default.
• W2600598352 cites W1998998050 @default.
• W2600598352 cites W1999928068 @default.
• W2600598352 cites W2004345651 @default.
• W2600598352 cites W2004378711 @default.
• W2600598352 cites W2005671770 @default.
• W2600598352 cites W2006334642 @default.
• W2600598352 cites W2006901907 @default.
• W2600598352 cites W2012806642 @default.
• W2600598352 cites W2017643163 @default.
• W2600598352 cites W2040646847 @default.
• W2600598352 cites W2047839366 @default.
• W2600598352 cites W2050892991 @default.
• W2600598352 cites W2052004158 @default.
• W2600598352 cites W2056279586 @default.
• W2600598352 cites W2056642013 @default.
• W2600598352 cites W2061586485 @default.
• W2600598352 cites W2062680061 @default.
• W2600598352 cites W2063614416 @default.
• W2600598352 cites W2064069006 @default.
• W2600598352 cites W2068412840 @default.
• W2600598352 cites W2073396389 @default.
• W2600598352 cites W2091449019 @default.
• W2600598352 cites W2106167453 @default.
• W2600598352 cites W2118930756 @default.
• W2600598352 cites W2131306660 @default.
• W2600598352 cites W2137385309 @default.
• W2600598352 cites W2138599948 @default.
• W2600598352 cites W2140472933 @default.
• W2600598352 cites W2141053537 @default.
• W2600598352 cites W2146645941 @default.
• W2600598352 cites W2147696224 @default.
• W2600598352 cites W2149223315 @default.
• W2600598352 cites W2151074487 @default.
• W2600598352 cites W2152022639 @default.
• W2600598352 cites W2317687805 @default.
• W2600598352 cites W2412416040 @default.
• W2600598352 doi "https://doi.org/10.1016/j.cellsig.2017.03.019" @default.
• W2600598352 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28347874" @default.
• W2600598352 hasPublicationYear "2017" @default.
• W2600598352 type Work @default.
• W2600598352 sameAs 2600598352 @default.
• W2600598352 citedByCount "11" @default.
• W2600598352 countsByYear W26005983522018 @default.
• W2600598352 countsByYear W26005983522019 @default.
• W2600598352 countsByYear W26005983522021 @default.
• W2600598352 countsByYear W26005983522022 @default.
• W2600598352 countsByYear W26005983522023 @default.
• W2600598352 crossrefType "journal-article" @default.
• W2600598352 hasAuthorship W2600598352A5002353034 @default.
• W2600598352 hasAuthorship W2600598352A5048416585 @default.
• W2600598352 hasAuthorship W2600598352A5050267628 @default.
• W2600598352 hasAuthorship W2600598352A5066212399 @default.
• W2600598352 hasAuthorship W2600598352A5082054461 @default.
• W2600598352 hasConcept C104317684 @default.
• W2600598352 hasConcept C11960822 @default.
• W2600598352 hasConcept C160225129 @default.
• W2600598352 hasConcept C1629964 @default.
• W2600598352 hasConcept C170493617 @default.
• W2600598352 hasConcept C2780019924 @default.
• W2600598352 hasConcept C55493867 @default.
• W2600598352 hasConcept C57074206 @default.
• W2600598352 hasConcept C57306754 @default.
• W2600598352 hasConcept C86339819 @default.
• W2600598352 hasConcept C86803240 @default.
• W2600598352 hasConcept C95444343 @default.
• W2600598352 hasConceptScore W2600598352C104317684 @default.
• W2600598352 hasConceptScore W2600598352C11960822 @default.
• W2600598352 hasConceptScore W2600598352C160225129 @default.

• next