Matches in SemOpenAlex for { <https://semopenalex.org/work/W2600693265> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2600693265 endingPage "349" @default.
- W2600693265 startingPage "348" @default.
- W2600693265 abstract "To regenerate the complex tissue such as bone-cartilage construct using tissue engineering approaches, controllable differentiation of mesenchymal stem cells (BMSCs) into chondrogenic and osteogenic lineages is crucially important. Although bilayered scaffolds have been investigated in vitro and in vivo, no culture system is available to test BMSCs differentiation into bone and cartilage simultaneously in bilayered scaffolds. This study investigated a defined culture media, which supported osteoblast and chondrocyte differentiation depending on growth factors implemented in biomaterials. In 2-dimensional culture, BMSCs differentiated to chondrocytes when transforming growth factor-beta 3 (TGF-β3) was added to the defined media, whereas osteogenic differentiation was induced by adding bone morphogenetic protein 7 (BMP-7). BMSC differentiation to osteogenic and chondrogenic lineages was further strengthen in 3-dimensional culture. Proteoglycan formation, type II collagen, and aggrecan were upregulated in the defined media when BMSCs were mixed with fibrin gel impregnated with TGF-β3. Mineralization and the expression of osteogenic markers such as alkaline phosphatase, osteopontin, and osteoclacin were noticeable when BMSCs cultured in hydroxyapatite-tricalcium phosphate (HA/TCP) scaffolds coated with BMP-7.This study generated and tested a growth media, which could induce osteogenic and chondrogenic differentiation of BMSCs in one culture system. These results will help the development of tissue substitutes for multi-complexed tissues such as subchondral replacement." @default.
- W2600693265 created "2017-04-07" @default.
- W2600693265 creator A5006427323 @default.
- W2600693265 creator A5017567855 @default.
- W2600693265 creator A5022337095 @default.
- W2600693265 creator A5050407874 @default.
- W2600693265 creator A5087431921 @default.
- W2600693265 date "2009-05-01" @default.
- W2600693265 modified "2023-09-27" @default.
- W2600693265 title "A CULTURE SYSTEM FOR THE DEVELOPMENT OF BONE-CARTILAGE COMPLEX" @default.
- W2600693265 hasPublicationYear "2009" @default.
- W2600693265 type Work @default.
- W2600693265 sameAs 2600693265 @default.
- W2600693265 citedByCount "0" @default.
- W2600693265 crossrefType "journal-article" @default.
- W2600693265 hasAuthorship W2600693265A5006427323 @default.
- W2600693265 hasAuthorship W2600693265A5017567855 @default.
- W2600693265 hasAuthorship W2600693265A5022337095 @default.
- W2600693265 hasAuthorship W2600693265A5050407874 @default.
- W2600693265 hasAuthorship W2600693265A5087431921 @default.
- W2600693265 hasConcept C104317684 @default.
- W2600693265 hasConcept C105702510 @default.
- W2600693265 hasConcept C136229726 @default.
- W2600693265 hasConcept C142724271 @default.
- W2600693265 hasConcept C148738053 @default.
- W2600693265 hasConcept C160160445 @default.
- W2600693265 hasConcept C181199279 @default.
- W2600693265 hasConcept C185592680 @default.
- W2600693265 hasConcept C197534660 @default.
- W2600693265 hasConcept C198826908 @default.
- W2600693265 hasConcept C202751555 @default.
- W2600693265 hasConcept C203014093 @default.
- W2600693265 hasConcept C204787440 @default.
- W2600693265 hasConcept C2776164576 @default.
- W2600693265 hasConcept C2780550940 @default.
- W2600693265 hasConcept C2780804394 @default.
- W2600693265 hasConcept C2781013607 @default.
- W2600693265 hasConcept C2781403057 @default.
- W2600693265 hasConcept C3020332539 @default.
- W2600693265 hasConcept C35496256 @default.
- W2600693265 hasConcept C49892992 @default.
- W2600693265 hasConcept C55493867 @default.
- W2600693265 hasConcept C71924100 @default.
- W2600693265 hasConcept C86803240 @default.
- W2600693265 hasConcept C95444343 @default.
- W2600693265 hasConceptScore W2600693265C104317684 @default.
- W2600693265 hasConceptScore W2600693265C105702510 @default.
- W2600693265 hasConceptScore W2600693265C136229726 @default.
- W2600693265 hasConceptScore W2600693265C142724271 @default.
- W2600693265 hasConceptScore W2600693265C148738053 @default.
- W2600693265 hasConceptScore W2600693265C160160445 @default.
- W2600693265 hasConceptScore W2600693265C181199279 @default.
- W2600693265 hasConceptScore W2600693265C185592680 @default.
- W2600693265 hasConceptScore W2600693265C197534660 @default.
- W2600693265 hasConceptScore W2600693265C198826908 @default.
- W2600693265 hasConceptScore W2600693265C202751555 @default.
- W2600693265 hasConceptScore W2600693265C203014093 @default.
- W2600693265 hasConceptScore W2600693265C204787440 @default.
- W2600693265 hasConceptScore W2600693265C2776164576 @default.
- W2600693265 hasConceptScore W2600693265C2780550940 @default.
- W2600693265 hasConceptScore W2600693265C2780804394 @default.
- W2600693265 hasConceptScore W2600693265C2781013607 @default.
- W2600693265 hasConceptScore W2600693265C2781403057 @default.
- W2600693265 hasConceptScore W2600693265C3020332539 @default.
- W2600693265 hasConceptScore W2600693265C35496256 @default.
- W2600693265 hasConceptScore W2600693265C49892992 @default.
- W2600693265 hasConceptScore W2600693265C55493867 @default.
- W2600693265 hasConceptScore W2600693265C71924100 @default.
- W2600693265 hasConceptScore W2600693265C86803240 @default.
- W2600693265 hasConceptScore W2600693265C95444343 @default.
- W2600693265 hasLocation W26006932651 @default.
- W2600693265 hasOpenAccess W2600693265 @default.
- W2600693265 hasPrimaryLocation W26006932651 @default.
- W2600693265 hasRelatedWork W1765143449 @default.
- W2600693265 hasRelatedWork W1977490791 @default.
- W2600693265 hasRelatedWork W1996200624 @default.
- W2600693265 hasRelatedWork W2005424476 @default.
- W2600693265 hasRelatedWork W2022790300 @default.
- W2600693265 hasRelatedWork W2037287554 @default.
- W2600693265 hasRelatedWork W2041859290 @default.
- W2600693265 hasRelatedWork W2044130204 @default.
- W2600693265 hasRelatedWork W2068636478 @default.
- W2600693265 hasRelatedWork W2122853611 @default.
- W2600693265 hasRelatedWork W2151751613 @default.
- W2600693265 hasRelatedWork W2153051769 @default.
- W2600693265 hasRelatedWork W2301137009 @default.
- W2600693265 hasRelatedWork W2570613324 @default.
- W2600693265 hasRelatedWork W2776486712 @default.
- W2600693265 hasRelatedWork W2897988174 @default.
- W2600693265 hasRelatedWork W2914810448 @default.
- W2600693265 hasRelatedWork W2970278108 @default.
- W2600693265 hasRelatedWork W2993874094 @default.
- W2600693265 hasRelatedWork W3024659829 @default.
- W2600693265 isParatext "false" @default.
- W2600693265 isRetracted "false" @default.
- W2600693265 magId "2600693265" @default.
- W2600693265 workType "article" @default.