FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2600797034> ?p ?o ?g. }

• W2600797034 endingPage "e0173978" @default.
• W2600797034 startingPage "e0173978" @default.
• W2600797034 abstract "Regenerative medicine requires, in many cases, physical supports to facilitate appropriate cellular architecture, cell polarization and the improvement of the correct differentiation processes of embryonic stem cells, induced pluripotent cells or adult cells. Because the interest in carbon nanomaterials has grown within the last decade in light of a wide variety of applications, the aim of this study was to test and evaluate the suitability and cytocompatibility of a particular nanometer-thin nanocrystalline glass-like carbon film (NGLC) composed of curved graphene flakes joined by an amorphous carbon matrix. This material is a disordered structure with high transparency and electrical conductivity. For this purpose, we used a cell line (SN4741) from substantia nigra dopaminergic cells derived from transgenic mouse embryos. Cells were cultured either in a powder of increasing concentrations of NGLC microflakes (82±37μm) in the medium or on top of nanometer-thin films bathed in the same culture medium. The metabolism activity of SN4741 cells in presence of NGLC was assessed using methylthiazolyldiphenyl-tetrazolium (MTT) and apoptosis/necrosis flow cytometry assay respectively. Growth and proliferation as well as senescence were demonstrated by western blot (WB) of proliferating cell nuclear antigen (PCNA), monoclonal phosphorylate Histone 3 (serine 10) (PH3) and SMP30 marker. Specific dopaminergic differentiation was confirmed by the WB analysis of tyrosine hydroxylase (TH). Cell maturation and neural capability were characterized using specific markers (SYP: synaptophysin and GIRK2: G-protein-regulated inward-rectifier potassium channel 2 protein) via immunofluorescence and coexistence measurements. The results demonstrated cell positive biocompatibility with different concentrations of NGLC. The cells underwent a process of adaptation of SN4741 cells to NGLC where their metabolism decreases. This process is related to a decrease of PH3 expression and significant increase SMP30 related to senescence processes. After 7 days, the cells increased the expression of TH and PCNA that is related to processes of DNA replication. On the other hand, cells cultured on top of the film showed axonal-like alignment, edge orientation, and network-like images after 7 days. Neuronal capability was demonstrated to a certain extent through the analysis of significant coexistence between SYP and GIRK2. Furthermore, we found a direct relationship between the thickness of the films and cell maturation. Although these findings share certain similarities to our previous findings with graphene oxide and its derivatives, this particular nanomaterial possesses the advantages of high conductivity and transparency. In conclusion, NGLC could represent a new platform for biomedical applications, such as for use in neural tissue engineering and biocompatible devices." @default.
• W2600797034 created "2017-04-07" @default.
• W2600797034 creator A5043351783 @default.
• W2600797034 creator A5048702156 @default.
• W2600797034 creator A5074682775 @default.
• W2600797034 creator A5081199549 @default.
• W2600797034 creator A5083044836 @default.
• W2600797034 date "2017-03-23" @default.
• W2600797034 modified "2023-10-18" @default.
• W2600797034 title "Cell survival and differentiation with nanocrystalline glass-like carbon using substantia nigra dopaminergic cells derived from transgenic mouse embryos" @default.
• W2600797034 cites W1502303178 @default.
• W2600797034 cites W1550402445 @default.
• W2600797034 cites W1819048918 @default.
• W2600797034 cites W1970511900 @default.
• W2600797034 cites W1971736084 @default.
• W2600797034 cites W1976887000 @default.
• W2600797034 cites W1989159677 @default.
• W2600797034 cites W1992371392 @default.
• W2600797034 cites W2005109726 @default.
• W2600797034 cites W2005113552 @default.
• W2600797034 cites W2008612285 @default.
• W2600797034 cites W2016181819 @default.
• W2600797034 cites W2019494208 @default.
• W2600797034 cites W2020823358 @default.
• W2600797034 cites W2027352256 @default.
• W2600797034 cites W2030449087 @default.
• W2600797034 cites W2031824752 @default.
• W2600797034 cites W2032431336 @default.
• W2600797034 cites W2045144284 @default.
• W2600797034 cites W2050869999 @default.
• W2600797034 cites W2051607882 @default.
• W2600797034 cites W2053815057 @default.
• W2600797034 cites W2072747624 @default.
• W2600797034 cites W2073545927 @default.
• W2600797034 cites W2077450385 @default.
• W2600797034 cites W2082647352 @default.
• W2600797034 cites W2085777643 @default.
• W2600797034 cites W2096255810 @default.
• W2600797034 cites W2101117526 @default.
• W2600797034 cites W2140386475 @default.
• W2600797034 cites W2141720090 @default.
• W2600797034 cites W2150737519 @default.
• W2600797034 cites W2161774007 @default.
• W2600797034 cites W2167279371 @default.
• W2600797034 cites W2186454889 @default.
• W2600797034 cites W2220679381 @default.
• W2600797034 cites W2235679284 @default.
• W2600797034 cites W22489932 @default.
• W2600797034 cites W2273559842 @default.
• W2600797034 cites W2315400560 @default.
• W2600797034 cites W2326664498 @default.
• W2600797034 cites W2398202111 @default.
• W2600797034 cites W2568294453 @default.
• W2600797034 cites W4251157576 @default.
• W2600797034 doi "https://doi.org/10.1371/journal.pone.0173978" @default.
• W2600797034 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5363826" @default.
• W2600797034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28334019" @default.
• W2600797034 hasPublicationYear "2017" @default.
• W2600797034 type Work @default.
• W2600797034 sameAs 2600797034 @default.
• W2600797034 citedByCount "9" @default.
• W2600797034 countsByYear W26007970342017 @default.
• W2600797034 countsByYear W26007970342018 @default.
• W2600797034 countsByYear W26007970342019 @default.
• W2600797034 countsByYear W26007970342020 @default.
• W2600797034 countsByYear W26007970342022 @default.
• W2600797034 crossrefType "journal-article" @default.
• W2600797034 hasAuthorship W2600797034A5043351783 @default.
• W2600797034 hasAuthorship W2600797034A5048702156 @default.
• W2600797034 hasAuthorship W2600797034A5074682775 @default.
• W2600797034 hasAuthorship W2600797034A5081199549 @default.
• W2600797034 hasAuthorship W2600797034A5083044836 @default.
• W2600797034 hasBestOaLocation W26007970341 @default.
• W2600797034 hasConcept C104317684 @default.
• W2600797034 hasConcept C137183658 @default.
• W2600797034 hasConcept C148738053 @default.
• W2600797034 hasConcept C153911025 @default.
• W2600797034 hasConcept C169760540 @default.
• W2600797034 hasConcept C185592680 @default.
• W2600797034 hasConcept C2780938664 @default.
• W2600797034 hasConcept C513476851 @default.
• W2600797034 hasConcept C55493867 @default.
• W2600797034 hasConcept C86803240 @default.
• W2600797034 hasConcept C95444343 @default.
• W2600797034 hasConceptScore W2600797034C104317684 @default.
• W2600797034 hasConceptScore W2600797034C137183658 @default.
• W2600797034 hasConceptScore W2600797034C148738053 @default.
• W2600797034 hasConceptScore W2600797034C153911025 @default.
• W2600797034 hasConceptScore W2600797034C169760540 @default.
• W2600797034 hasConceptScore W2600797034C185592680 @default.
• W2600797034 hasConceptScore W2600797034C2780938664 @default.
• W2600797034 hasConceptScore W2600797034C513476851 @default.
• W2600797034 hasConceptScore W2600797034C55493867 @default.
• W2600797034 hasConceptScore W2600797034C86803240 @default.
• W2600797034 hasConceptScore W2600797034C95444343 @default.
• W2600797034 hasFunder F4320322930 @default.
• W2600797034 hasIssue "3" @default.
• W2600797034 hasLocation W26007970341 @default.

• next