Matches in SemOpenAlex for { <https://semopenalex.org/work/W2600850647> ?p ?o ?g. }
- W2600850647 endingPage "2619" @default.
- W2600850647 startingPage "2609" @default.
- W2600850647 abstract "Polycationic polymers and DNA form soluble complexes in aqueous solution, which allows the transfer of genetic material into cells. Therefore, these chemically-modified polymers are of interest for use in studies aimed at better transfection efficiency and gene expression along with reduced cytotoxicity. In this study, branched poly(ethylene imine) (PEI) was modified by alkylation with n-alkyl groups (n = 4, 6, 8 and 12 carbons). The polyplexes formed through interaction of the modified PEIs and calf thymus DNA (ctDNA) were investigated using UV-Vis spectrophotometry, ethidium bromide fluorescence emission, circular dichroism spectroscopy, dynamic light scattering, and small-angle X-ray scattering techniques along with the determination of the zeta potential and viscosity. According to the results obtained, the formation of ctDNA–PEI polyplexes occurs in three steps. Firstly, when a small amount of polyelectrolyte is present the ctDNA chains are partially compacted. Subsequently, with the addition of more polyelectrolyte, the complexes have a null charge density and micrometric size. Lastly, with a higher concentration of PEI, the ctDNA is fully compacted by the PEI chains, leading to positively charged complexes with Rh values in the range of 52.0–86.0 nm. The viscosity and SAXS analysis suggested that the unmodified PEI exhibits the strongest interaction and promotes the best ctDNA condensation." @default.
- W2600850647 created "2017-04-07" @default.
- W2600850647 creator A5006705850 @default.
- W2600850647 creator A5033004630 @default.
- W2600850647 creator A5071074802 @default.
- W2600850647 creator A5086945862 @default.
- W2600850647 date "2017-01-01" @default.
- W2600850647 modified "2023-10-18" @default.
- W2600850647 title "Properties of polyplexes formed through interaction between hydrophobically-modified poly(ethylene imine)s and calf thymus DNA in aqueous solution" @default.
- W2600850647 cites W1973458116 @default.
- W2600850647 cites W1973892257 @default.
- W2600850647 cites W1975046421 @default.
- W2600850647 cites W1981734925 @default.
- W2600850647 cites W1990651273 @default.
- W2600850647 cites W1995134600 @default.
- W2600850647 cites W2000925146 @default.
- W2600850647 cites W2002061266 @default.
- W2600850647 cites W2012605911 @default.
- W2600850647 cites W2012766567 @default.
- W2600850647 cites W2017307273 @default.
- W2600850647 cites W2020002410 @default.
- W2600850647 cites W2021627241 @default.
- W2600850647 cites W2025729523 @default.
- W2600850647 cites W2027310997 @default.
- W2600850647 cites W2037595752 @default.
- W2600850647 cites W2039577598 @default.
- W2600850647 cites W2041249432 @default.
- W2600850647 cites W2042863693 @default.
- W2600850647 cites W2046361197 @default.
- W2600850647 cites W2049358561 @default.
- W2600850647 cites W2050033753 @default.
- W2600850647 cites W2054547436 @default.
- W2600850647 cites W2059777511 @default.
- W2600850647 cites W2063268753 @default.
- W2600850647 cites W2069717129 @default.
- W2600850647 cites W2069720087 @default.
- W2600850647 cites W2073134929 @default.
- W2600850647 cites W2083584116 @default.
- W2600850647 cites W2084712756 @default.
- W2600850647 cites W2086752889 @default.
- W2600850647 cites W2087484535 @default.
- W2600850647 cites W2087497808 @default.
- W2600850647 cites W2092871360 @default.
- W2600850647 cites W2106005174 @default.
- W2600850647 cites W2116609429 @default.
- W2600850647 cites W2120594328 @default.
- W2600850647 cites W2129569835 @default.
- W2600850647 cites W2141815104 @default.
- W2600850647 cites W2144312129 @default.
- W2600850647 cites W2152866362 @default.
- W2600850647 cites W2154329741 @default.
- W2600850647 cites W2163921171 @default.
- W2600850647 cites W2185394761 @default.
- W2600850647 cites W2185714801 @default.
- W2600850647 cites W2190427975 @default.
- W2600850647 cites W2191250305 @default.
- W2600850647 cites W2206702281 @default.
- W2600850647 cites W2224657033 @default.
- W2600850647 cites W2244782642 @default.
- W2600850647 cites W2297794140 @default.
- W2600850647 cites W2344322333 @default.
- W2600850647 cites W2488742747 @default.
- W2600850647 cites W2495349537 @default.
- W2600850647 cites W2562187931 @default.
- W2600850647 cites W275348111 @default.
- W2600850647 doi "https://doi.org/10.1039/c6sm02835g" @default.
- W2600850647 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28327732" @default.
- W2600850647 hasPublicationYear "2017" @default.
- W2600850647 type Work @default.
- W2600850647 sameAs 2600850647 @default.
- W2600850647 citedByCount "5" @default.
- W2600850647 countsByYear W26008506472019 @default.
- W2600850647 countsByYear W26008506472020 @default.
- W2600850647 countsByYear W26008506472021 @default.
- W2600850647 countsByYear W26008506472022 @default.
- W2600850647 countsByYear W26008506472023 @default.
- W2600850647 crossrefType "journal-article" @default.
- W2600850647 hasAuthorship W2600850647A5006705850 @default.
- W2600850647 hasAuthorship W2600850647A5033004630 @default.
- W2600850647 hasAuthorship W2600850647A5071074802 @default.
- W2600850647 hasAuthorship W2600850647A5086945862 @default.
- W2600850647 hasConcept C104317684 @default.
- W2600850647 hasConcept C115028873 @default.
- W2600850647 hasConcept C120665830 @default.
- W2600850647 hasConcept C121332964 @default.
- W2600850647 hasConcept C131888329 @default.
- W2600850647 hasConcept C133571119 @default.
- W2600850647 hasConcept C14631669 @default.
- W2600850647 hasConcept C153356533 @default.
- W2600850647 hasConcept C155672457 @default.
- W2600850647 hasConcept C15920480 @default.
- W2600850647 hasConcept C161790260 @default.
- W2600850647 hasConcept C171250308 @default.
- W2600850647 hasConcept C178790620 @default.
- W2600850647 hasConcept C184651966 @default.
- W2600850647 hasConcept C185592680 @default.
- W2600850647 hasConcept C188027245 @default.
- W2600850647 hasConcept C191486275 @default.