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• W2601113669 startingPage "719" @default.
• W2601113669 abstract "Background and Objective To study the mechanisms of human glioblastoma cell resistance to methyl ester pyropheophorbide‐a‐mediated photodynamic therapy (MPPa‐PDT) and the relationship between the cells and adenosine triphosphate‐binding cassette superfamily G member 2 (ABCG2). Study Design/Materials and Methods The sensitivity of four human glioma cell lines (U87, A172, SHG‐44, and U251) to MPPa‐PDT was detected with a CCK‐8 assay. Cell apoptosis, intracellular MPPa, and singlet oxygen were tested with flow cytometry. The mRNA and protein expression of ATP‐binding cassette transporters (ABCG2, MRP1, and MDR1) were detected by PCR and Western blot, respectively. Results Both the sensitivity to MPPa‐PDT and intracellular MPPa in A172 were the lowest among the four cell lines, while expression of ABCG2 mRNA and protein in A172 were the highest. The intracellular MPPa and ROS in A172 receiving MPPa‐PDT significantly increased after using the ABCG2 inhibitor fumitremorgin C (FTC). Both cell viability and apoptosis in A172 cells undergoing MPPa‐PDT were significantly improved with FTC. Conclusions ABCG2 plays a significant role in the resistance of A172 to MPPa‐PDT. Lasers Surg. Med. 49:719–726, 2017. © 2017 Wiley Periodicals, Inc." @default.
• W2601113669 created "2017-04-07" @default.
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• W2601113669 date "2017-03-29" @default.
• W2601113669 modified "2023-10-16" @default.
• W2601113669 title "The sensitivity of glioma cells to pyropheophorbide-αmethyl ester-mediated photodynamic therapy is enhanced by inhibiting ABCG2" @default.
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• W2601113669 doi "https://doi.org/10.1002/lsm.22661" @default.
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