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- W2601227130 abstract "In this study, the catalytic efficiency using NitA from Acidovorax facilis 72W for nicotinic acid (NA) production was investigated and further improved by site-directed mutagenesis. Results showed that the specific activity of mutated NitA-C2 (F168V-S192F) towards 3-cyanopyridine increased 5-fold to 35 U mg−1 protein. Further characterization of the biochemical properties of both nitrilases showed the optimal pH and temperature were 6.0–8.0 and 60 °C, respectively, whereas the pH and thermal stability of NitA-C2 were decreased. Finally, whole cell catalysis was adopted for NA production and a 100% conversion yield was achieved under 0.1 mol L−1 3-cyanopyridine for both strains. Besides, the conversion rate by E. coli BL21 (DE3-pET-nitA-C2) reached to 1.0 mmol min−1 g−1 wet cell weight, which was 3-fold higher than that by E. coli BL21 (DE3-pET-nitA). These results indicated that the mutated NitA-C2 was a promising candidate which holds potential application in biological NA production." @default.
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- W2601227130 date "2016-11-01" @default.
- W2601227130 modified "2023-10-17" @default.
- W2601227130 title "Enhanced catalytic efficiency of nitrilase from Acidovorax facilis 72W and application in bioconversion of 3-cyanopyridine to nicotinic acid" @default.
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- W2601227130 doi "https://doi.org/10.1016/j.molcatb.2017.03.010" @default.
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