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• W2601547100 abstract "Small non-coding RNAs (sRNAs) are involved in the control of numerous cellular processes through various regulatory mechanisms, and in the past decade many studies have identified sRNAs in a multitude of bacterial species using RNA sequencing (RNA-seq). Here, we present the first genome-wide analysis of sRNA sequencing data in Rhodobacter capsulatus, a purple nonsulfur photosynthetic alphaproteobacterium. Using a recently developed bioinformatics approach, sRNA-Detect, we detected 422 putative sRNAs from R. capsulatus RNA-seq data. Based on their sequence similarity to sRNAs in a sRNA collection, consisting of published putative sRNAs from 23 additional bacterial species, and RNA databases, the sequences of 124 putative sRNAs were conserved in at least one other bacterial species; and, 19 putative sRNAs were assigned a predicted function. We bioinformatically characterized all putative sRNAs and applied machine learning approaches to calculate the probability of a nucleotide sequence to be a bona fide sRNA. The resulting quantitative model was able to correctly classify 95.2% of sequences in a validation set. We found that putative cis-targets for antisense and partially overlapping sRNAs were enriched with protein-coding genes involved in primary metabolic processes, photosynthesis, compound binding, and with genes forming part of macromolecular complexes. We performed differential expression analysis to compare the wild type strain to a mutant lacking the response regulator CtrA, an important regulator of gene expression in R. capsulatus, and identified 18 putative sRNAs with differing levels in the two strains. Finally, we validated the existence and expression patterns of four novel sRNAs by Northern blot analysis." @default.
• W2601547100 created "2017-04-07" @default.
• W2601547100 creator A5009912214 @default.
• W2601547100 creator A5042983284 @default.
• W2601547100 creator A5044078900 @default.
• W2601547100 creator A5061532452 @default.
• W2601547100 date "2017-04-28" @default.
• W2601547100 modified "2023-10-14" @default.
• W2601547100 title "Genome-wide identification and characterization of small RNAs in <i>Rhodobacter capsulatus</i> and identification of small RNAs affected by loss of the response regulator CtrA" @default.
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• W2601547100 doi "https://doi.org/10.1080/15476286.2017.1306175" @default.
• W2601547100 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5546546" @default.
• W2601547100 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28296577" @default.
• W2601547100 hasPublicationYear "2017" @default.
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