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• W2601846112 abstract "Abstract Pooled CRISPR/Cas9 screens are a powerful and versatile tool for the systematic investigation of cellular processes in a variety of organisms. Such screens generate large amounts of data that present a new challenge to analyze and interpret. Here, we developed a web application to analyze, document and explore pooled CRISR/Cas9 screens using a unified single workflow. The end-to-end analysis pipeline features eight different hit calling strategies based on state-of-the-art methods, including DESeq2, MAGeCK, edgeR, sgRSEA, Z-Ratio, Mann-Whitney test, ScreenBEAM and BAGEL. Results can be compared with interactive visualizations and data tables. CRISPRAnalyzeR integrates meta-information from 26 external data resources, providing a wide array of options for the annotation and documentation of screens. The application was developed with user experience in mind, requiring no previous knowledge in bioinformatics. All modern operating systems are supported. Availability and online documentation: The source code, a pre-configured docker application, sample data and a documentation can be found on our GitHub page ( http://www.github.com/boutroslab/CRISPRAnalyzeR ). A tutorial video can be found at http://www.crispr-analyzer.org." @default.
• W2601846112 created "2017-04-07" @default.
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• W2601846112 date "2017-02-20" @default.
• W2601846112 modified "2023-10-16" @default.
• W2601846112 title "CRISPRAnalyzeR: Interactive analysis, annotation and documentation of pooled CRISPR screens" @default.
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• W2601846112 doi "https://doi.org/10.1101/109967" @default.
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