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- W2602784731 abstract "The effect of overexpression of endogenous delta-6 fatty acid desaturase gene (ER<DELTA>6FAD) on eicosapentaenoic acid (EPA) production and total lipid content was investigated in Phaeodactylum tricornutum. All three randomly selected transformants exhibited significant increase (47.66%) in their EPA content, which reached up to 38.101 mg/g DW in algal strain D63. The total lipid content of all the three transformants was significantly higher (16.40–18.64%) than that of the wild-type strain. These findings suggested that the reaction catalyzed by ER<DELTA>6FAD is a limiting step for EPA biosynthesis, overexpression of endogenous ER<DELTA>6FAD gene is an effective method for improving EPA production in eukaryotic microalgae, and fatty acid metabolic pathway in microalgae can be genetically modified." @default.
- W2602784731 created "2017-04-07" @default.
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- W2602784731 date "2017-06-01" @default.
- W2602784731 modified "2023-10-17" @default.
- W2602784731 title "Overexpression of endogenous delta-6 fatty acid desaturase gene enhances eicosapentaenoic acid accumulation in Phaeodactylum tricornutum" @default.
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- W2602784731 doi "https://doi.org/10.1016/j.procbio.2017.03.013" @default.
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