Matches in SemOpenAlex for { <https://semopenalex.org/work/W2602882036> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2602882036 abstract "e18083 Background: Identical lung cancer cells may get diversified depending on tumor microenvironments. We hypothesized that diversity of cancer associated fibroblasts renders identical lung cancer cells various tumor characteristics such as drug responsiveness and proliferation rate. To test this hypothesis, we isolated cancer associated fibroblasts from 20 different lung cancers, and compared their effects on the proliferation and drug responsiveness of lung cell lines. Methods: Cancer associated fibroblasts were isolated from 20 solid tumors of NSCLCs, and treated with Mitomycin to control the overgrowth during cocultures. H358 and A549 cells were mixed with cancer associated fibroblasts in 1:1 ratio and co-cultured for 4-7 days. Cell number was scored by time-lapse photography, and cell morphology was observed under fluorescent microscope. Expression of cell type markers were examined. Responsiveness to Erlotinib was examined in coculture condistions. Results: Cancer associated fibroblasts stimulated cancer cell proliferation ranging from almost no effects to approximately 3 folds. Coculture in transwell with no direct contacts with cancer cells showed minimal stimulatory effects. Direct contacts induced dramatic changes in cancer cell morphology from cohesive epithelial colonies to dispersed fibroblastic single cells. EMT markers such as down-regulation of Cytokeratins and up-regulation of Vimentin were detected in GFP positive cells. A549 cells that were sensitive to 15 uM Erlotinib (IC50=7.5uM) became resistant when co-cultured with cancer associated fibroblasts. Conclusions: In this study, cancer associated fibroblasts isolated from different lung cancers induced significant changes in tumor cell characteristics such as proliferation, EMT, and drug responsiveness." @default.
- W2602882036 created "2017-04-07" @default.
- W2602882036 creator A5010364211 @default.
- W2602882036 creator A5061651749 @default.
- W2602882036 creator A5066848151 @default.
- W2602882036 creator A5086614178 @default.
- W2602882036 date "2012-05-20" @default.
- W2602882036 modified "2023-09-27" @default.
- W2602882036 title "Effect of cancer-associated fibroblasts on cell proliferation and resistance to EGFR-TKI in direct coculture with lung cancer cell lines." @default.
- W2602882036 doi "https://doi.org/10.1200/jco.2012.30.15_suppl.e18083" @default.
- W2602882036 hasPublicationYear "2012" @default.
- W2602882036 type Work @default.
- W2602882036 sameAs 2602882036 @default.
- W2602882036 citedByCount "0" @default.
- W2602882036 crossrefType "journal-article" @default.
- W2602882036 hasAuthorship W2602882036A5010364211 @default.
- W2602882036 hasAuthorship W2602882036A5061651749 @default.
- W2602882036 hasAuthorship W2602882036A5066848151 @default.
- W2602882036 hasAuthorship W2602882036A5086614178 @default.
- W2602882036 hasConcept C121608353 @default.
- W2602882036 hasConcept C126322002 @default.
- W2602882036 hasConcept C143998085 @default.
- W2602882036 hasConcept C1491633281 @default.
- W2602882036 hasConcept C2776256026 @default.
- W2602882036 hasConcept C2780381497 @default.
- W2602882036 hasConcept C2909706936 @default.
- W2602882036 hasConcept C502942594 @default.
- W2602882036 hasConcept C54355233 @default.
- W2602882036 hasConcept C62112901 @default.
- W2602882036 hasConcept C71924100 @default.
- W2602882036 hasConcept C81885089 @default.
- W2602882036 hasConcept C86803240 @default.
- W2602882036 hasConcept C96232424 @default.
- W2602882036 hasConceptScore W2602882036C121608353 @default.
- W2602882036 hasConceptScore W2602882036C126322002 @default.
- W2602882036 hasConceptScore W2602882036C143998085 @default.
- W2602882036 hasConceptScore W2602882036C1491633281 @default.
- W2602882036 hasConceptScore W2602882036C2776256026 @default.
- W2602882036 hasConceptScore W2602882036C2780381497 @default.
- W2602882036 hasConceptScore W2602882036C2909706936 @default.
- W2602882036 hasConceptScore W2602882036C502942594 @default.
- W2602882036 hasConceptScore W2602882036C54355233 @default.
- W2602882036 hasConceptScore W2602882036C62112901 @default.
- W2602882036 hasConceptScore W2602882036C71924100 @default.
- W2602882036 hasConceptScore W2602882036C81885089 @default.
- W2602882036 hasConceptScore W2602882036C86803240 @default.
- W2602882036 hasConceptScore W2602882036C96232424 @default.
- W2602882036 hasLocation W26028820361 @default.
- W2602882036 hasOpenAccess W2602882036 @default.
- W2602882036 hasPrimaryLocation W26028820361 @default.
- W2602882036 hasRelatedWork W2168828515 @default.
- W2602882036 hasRelatedWork W2234096677 @default.
- W2602882036 hasRelatedWork W2313149101 @default.
- W2602882036 hasRelatedWork W2318325393 @default.
- W2602882036 hasRelatedWork W2329884926 @default.
- W2602882036 hasRelatedWork W2342331352 @default.
- W2602882036 hasRelatedWork W2371113204 @default.
- W2602882036 hasRelatedWork W2413436515 @default.
- W2602882036 hasRelatedWork W2550880770 @default.
- W2602882036 hasRelatedWork W2580764669 @default.
- W2602882036 hasRelatedWork W2727025454 @default.
- W2602882036 hasRelatedWork W2792983660 @default.
- W2602882036 hasRelatedWork W2848305443 @default.
- W2602882036 hasRelatedWork W2905132531 @default.
- W2602882036 hasRelatedWork W2914550341 @default.
- W2602882036 hasRelatedWork W2916709334 @default.
- W2602882036 hasRelatedWork W2946109722 @default.
- W2602882036 hasRelatedWork W29743144 @default.
- W2602882036 hasRelatedWork W3187253705 @default.
- W2602882036 hasRelatedWork W3036915469 @default.
- W2602882036 isParatext "false" @default.
- W2602882036 isRetracted "false" @default.
- W2602882036 magId "2602882036" @default.
- W2602882036 workType "article" @default.