FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2603416299> ?p ?o ?g. }

• W2603416299 endingPage "13" @default.
• W2603416299 startingPage "6" @default.
• W2603416299 abstract "Porcine epidemic diarrhea virus (PEDV) is an emerging pathogenic coronavirus that causes a significant economic burden to the swine industry. The virus infects the intestinal epithelium and causes villous atrophy, resulting in diarrhea and dehydration. Interaction of the viral spike (S) surface glycoprotein − through its S1 subunit − with the host cell receptor is the first step in infection and the main determinant for virus tropism. As for several other alphacoronaviruses including the porcine transmissible gastroenteritis virus (TGEV) and the human coronavirus 229E (HCoV-229E), the aminopeptidase N (APN) protein was reported to be a functional receptor for PEDV. In this study we examined the role of APN as a receptor. We show that overexpression of porcine APN renders MDCK cells susceptible to TGEV, but not to PEDV. Consistently, unlike TGEV-S1, PEDV-S1 exhibited no binding to cell-surface expressed APN or to a soluble version of APN. Moreover, preincubation of these viruses with soluble APN or pretreatment of APN expressing ST cells with soluble TGEV-S1 blocked TGEV infection, but had no effect on infection by PEDV. The combined observations indicated that APN is not required for PEDV infection. To definitively prove this conclusion, we applied CRISPR/Cas9 genome engineering to knock out APN expression in PEDV-susceptible porcine (ST) and human cell lines (Huh7 and HeLa). As a consequence these cells no longer bound TGEV-S1 and HCoV-229E-S1 at their surface and were resistant to infection by the corresponding viruses. However, genetic ablation of APN expression had no effect on their infectability by PEDV, demonstrating that APN is not essential for PEDV cell entry." @default.
• W2603416299 created "2017-04-07" @default.
• W2603416299 creator A5000266713 @default.
• W2603416299 creator A5008540204 @default.
• W2603416299 creator A5033046679 @default.
• W2603416299 creator A5040714564 @default.
• W2603416299 creator A5054220107 @default.
• W2603416299 creator A5080132990 @default.
• W2603416299 date "2017-05-01" @default.
• W2603416299 modified "2023-10-14" @default.
• W2603416299 title "Aminopeptidase N is not required for porcine epidemic diarrhea virus cell entry" @default.
• W2603416299 cites W109443668 @default.
• W2603416299 cites W1542641435 @default.
• W2603416299 cites W1554543951 @default.
• W2603416299 cites W1584250393 @default.
• W2603416299 cites W1920404179 @default.
• W2603416299 cites W1966238900 @default.
• W2603416299 cites W1979615660 @default.
• W2603416299 cites W1983342808 @default.
• W2603416299 cites W1985255881 @default.
• W2603416299 cites W1988724422 @default.
• W2603416299 cites W2006785079 @default.
• W2603416299 cites W2012504769 @default.
• W2603416299 cites W2019410209 @default.
• W2603416299 cites W2022347066 @default.
• W2603416299 cites W2027518730 @default.
• W2603416299 cites W2029627660 @default.
• W2603416299 cites W2032830483 @default.
• W2603416299 cites W2042197160 @default.
• W2603416299 cites W2064350446 @default.
• W2603416299 cites W2068707110 @default.
• W2603416299 cites W2075213182 @default.
• W2603416299 cites W2075944727 @default.
• W2603416299 cites W2082183492 @default.
• W2603416299 cites W2086955313 @default.
• W2603416299 cites W2089061811 @default.
• W2603416299 cites W2094023848 @default.
• W2603416299 cites W2096261947 @default.
• W2603416299 cites W2106314610 @default.
• W2603416299 cites W2106713698 @default.
• W2603416299 cites W2112533943 @default.
• W2603416299 cites W2119111857 @default.
• W2603416299 cites W2134464188 @default.
• W2603416299 cites W2134972446 @default.
• W2603416299 cites W2137268987 @default.
• W2603416299 cites W2149330802 @default.
• W2603416299 cites W2151546656 @default.
• W2603416299 cites W2154988647 @default.
• W2603416299 cites W2156639337 @default.
• W2603416299 cites W2165907695 @default.
• W2603416299 cites W2168359445 @default.
• W2603416299 cites W2170463984 @default.
• W2603416299 cites W2273355031 @default.
• W2603416299 cites W2278619219 @default.
• W2603416299 cites W2331071821 @default.
• W2603416299 cites W2431224648 @default.
• W2603416299 cites W2442899697 @default.
• W2603416299 cites W2471957175 @default.
• W2603416299 cites W2498036824 @default.
• W2603416299 cites W42425353 @default.
• W2603416299 cites W4248552215 @default.
• W2603416299 doi "https://doi.org/10.1016/j.virusres.2017.03.018" @default.
• W2603416299 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7114539" @default.
• W2603416299 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28363778" @default.
• W2603416299 hasPublicationYear "2017" @default.
• W2603416299 type Work @default.
• W2603416299 sameAs 2603416299 @default.
• W2603416299 citedByCount "68" @default.
• W2603416299 countsByYear W26034162992017 @default.
• W2603416299 countsByYear W26034162992018 @default.
• W2603416299 countsByYear W26034162992019 @default.
• W2603416299 countsByYear W26034162992020 @default.
• W2603416299 countsByYear W26034162992021 @default.
• W2603416299 countsByYear W26034162992022 @default.
• W2603416299 countsByYear W26034162992023 @default.
• W2603416299 crossrefType "journal-article" @default.
• W2603416299 hasAuthorship W2603416299A5000266713 @default.
• W2603416299 hasAuthorship W2603416299A5008540204 @default.
• W2603416299 hasAuthorship W2603416299A5033046679 @default.
• W2603416299 hasAuthorship W2603416299A5040714564 @default.
• W2603416299 hasAuthorship W2603416299A5054220107 @default.
• W2603416299 hasAuthorship W2603416299A5080132990 @default.
• W2603416299 hasBestOaLocation W26034162991 @default.
• W2603416299 hasConcept C142724271 @default.
• W2603416299 hasConcept C159047783 @default.
• W2603416299 hasConcept C2522874641 @default.
• W2603416299 hasConcept C2775862345 @default.
• W2603416299 hasConcept C2776230035 @default.
• W2603416299 hasConcept C2777648638 @default.
• W2603416299 hasConcept C2779134260 @default.
• W2603416299 hasConcept C3008058167 @default.
• W2603416299 hasConcept C33602714 @default.
• W2603416299 hasConcept C35693153 @default.
• W2603416299 hasConcept C524204448 @default.
• W2603416299 hasConcept C54355233 @default.
• W2603416299 hasConcept C71924100 @default.
• W2603416299 hasConcept C81885089 @default.
• W2603416299 hasConcept C86803240 @default.

• next