Matches in SemOpenAlex for { <https://semopenalex.org/work/W2603677297> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2603677297 abstract "Antibiotic resistance is increasing worldwide, and is considered a serious threat to public health by e.g. the World Health Organization. Antibiotic resistance genes are hypothesized to originate from harmless bacteria in and around us, from where they are horizontally transfered into human pathogens. It is therefore of great importance to explore human-associated and environmental bacterial communities to identify novel antibiotic resistance genes before they reach clinical settings. The three papers presented in this thesis aim to identify novel antibiotic resistance genes in large genomic and metagenomic datasets. In paper I , the aim was to identify novel genes of the clinically important subclass B1 metallo-β-lactamases. By analyzing whole bacterial genomes as well as metagenomes from environmental and human-associated bacterial communities, 76 novel putative B1 genes were predicted. Twenty-one of these were selected for experimental validation, whereof 18 expressed the predicted phenotype in E. coli . Phylogentic analysis revealed that the novel genes formed 59 previously undescribed gene families. In paper II , a large volume of genomic and metagenomic data was searched for novel plasmid-mediated quinolone resistance ( qnr ) genes. In total, 611 qnr genes were predicted, of which 20 were putative novel. Nine of these were experimentally tested in E. coli , whereof eight expressed the predicted phenotype. In paper III , a new method for identification and reconstruction of novel antibiotic resistance genes from fragmented metagenomic data was presented. The method is based on gene specific models, which are optimized for a high sensitivity and specificity. The method is furthermore computationally efficient and can be applied to any class of resistance genes. The results of this thesis provides a deeper insight to the diversity and evolutionary history of two types of clinically relevant antibiotic resistance genes. It also provides new methods for efficient and reliable identification of novel resistance genes in fragmented metagenomic data." @default.
- W2603677297 created "2017-04-07" @default.
- W2603677297 creator A5078157638 @default.
- W2603677297 date "2017-01-01" @default.
- W2603677297 modified "2023-09-27" @default.
- W2603677297 title "Identification of novel antibiotic resistance genes through large-scale data analysis" @default.
- W2603677297 hasPublicationYear "2017" @default.
- W2603677297 type Work @default.
- W2603677297 sameAs 2603677297 @default.
- W2603677297 citedByCount "0" @default.
- W2603677297 crossrefType "dissertation" @default.
- W2603677297 hasAuthorship W2603677297A5078157638 @default.
- W2603677297 hasConcept C104317684 @default.
- W2603677297 hasConcept C116834253 @default.
- W2603677297 hasConcept C127716648 @default.
- W2603677297 hasConcept C141231307 @default.
- W2603677297 hasConcept C15151743 @default.
- W2603677297 hasConcept C22744801 @default.
- W2603677297 hasConcept C501593827 @default.
- W2603677297 hasConcept C54355233 @default.
- W2603677297 hasConcept C59822182 @default.
- W2603677297 hasConcept C70721500 @default.
- W2603677297 hasConcept C86803240 @default.
- W2603677297 hasConcept C94665300 @default.
- W2603677297 hasConceptScore W2603677297C104317684 @default.
- W2603677297 hasConceptScore W2603677297C116834253 @default.
- W2603677297 hasConceptScore W2603677297C127716648 @default.
- W2603677297 hasConceptScore W2603677297C141231307 @default.
- W2603677297 hasConceptScore W2603677297C15151743 @default.
- W2603677297 hasConceptScore W2603677297C22744801 @default.
- W2603677297 hasConceptScore W2603677297C501593827 @default.
- W2603677297 hasConceptScore W2603677297C54355233 @default.
- W2603677297 hasConceptScore W2603677297C59822182 @default.
- W2603677297 hasConceptScore W2603677297C70721500 @default.
- W2603677297 hasConceptScore W2603677297C86803240 @default.
- W2603677297 hasConceptScore W2603677297C94665300 @default.
- W2603677297 hasLocation W26036772971 @default.
- W2603677297 hasOpenAccess W2603677297 @default.
- W2603677297 hasPrimaryLocation W26036772971 @default.
- W2603677297 hasRelatedWork W1515007698 @default.
- W2603677297 hasRelatedWork W1986618222 @default.
- W2603677297 hasRelatedWork W2004547151 @default.
- W2603677297 hasRelatedWork W206155369 @default.
- W2603677297 hasRelatedWork W2095711442 @default.
- W2603677297 hasRelatedWork W2158753659 @default.
- W2603677297 hasRelatedWork W2163421806 @default.
- W2603677297 hasRelatedWork W2460324897 @default.
- W2603677297 hasRelatedWork W2587678577 @default.
- W2603677297 hasRelatedWork W2773835172 @default.
- W2603677297 hasRelatedWork W2793867488 @default.
- W2603677297 hasRelatedWork W2807096210 @default.
- W2603677297 hasRelatedWork W2900201984 @default.
- W2603677297 hasRelatedWork W2967125766 @default.
- W2603677297 hasRelatedWork W2980278798 @default.
- W2603677297 hasRelatedWork W3004432693 @default.
- W2603677297 hasRelatedWork W3047666078 @default.
- W2603677297 hasRelatedWork W3139121571 @default.
- W2603677297 hasRelatedWork W3146514680 @default.
- W2603677297 hasRelatedWork W2415725844 @default.
- W2603677297 isParatext "false" @default.
- W2603677297 isRetracted "false" @default.
- W2603677297 magId "2603677297" @default.
- W2603677297 workType "dissertation" @default.