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- W2603760993 abstract "Abstract The present study aimed to investigate the role of magnitude in adaptive response of osteoblasts exposed to compressive stress. Murine primary osteoblasts and MC3T3-E1 cells were exposed to compressive stress (0, 1, 2, 3, 4, and 5 g/cm 2 ) in 3D culture. Cell viability was evaluated, and expression levels of Runx2, Alp, Ocn, Rankl , and Opg were examined. ALP activity in osteoblasts and TRAP activity in RAW264.7 cells co-cultured with MC3T3-E1 cells were assayed. Results showed that compressive stress within 5.0 g/cm 2 did not influence cell viability. Both osteoblastic and osteoblast-regulated osteoclastic differentiation were enhanced at 2 g/cm 2 . An increase in stress above 2 g/cm 2 did not enhance osteoblastic differentiation further but significantly inhibited osteoblast-regualted osteoclastic differentiation. This study suggested that compressive stress regulates osteoblastic and osteoclastic differentiation through osteoblasts in a magnitude-dependent manner." @default.
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- W2603760993 date "2017-03-20" @default.
- W2603760993 modified "2023-09-26" @default.
- W2603760993 title "Magnitude-dependent response of osteoblasts regulated by compressive stress" @default.
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- W2603760993 doi "https://doi.org/10.1038/srep44925" @default.
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