FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2603910151> ?p ?o ?g. }

• W2603910151 endingPage "1902" @default.
• W2603910151 startingPage "1899" @default.
• W2603910151 abstract "ABSTRACT A desirable feature of many therapeutic glycoprotein production processes is to maximize the final sialic acid content. In this study, the effect of applying a novel chemical analog of the sialic acid precursor N‐acetylmannosamine (ManNAc) on the sialic acid content of cellular proteins and a model recombinant glycoprotein, erythropoietin (EPO), was investigated in CHO‐K1 cells. By introducing the 1,3,4‐ O ‐Bu 3 ManNAc analog at 200–300 µM into cell culture media, the intracellular sialic acid content of EPO‐expressing cells increased ∼8‐fold over untreated controls while the level of cellular sialylated glycoconjugates increased significantly as well. For example, addition of 200–300 µM 1,3,4‐ O ‐Bu 3 ManNAc resulted in >40% increase in final sialic acid content of recombinant EPO, while natural ManNAc at ∼100 times higher concentration of 20 mM produced a less profound change in EPO sialylation. Collectively, these results indicate that butyrate‐derivatization of ManNAc improves the capacity of cells to incorporate exogenous ManNAc into the sialic acid biosynthetic pathway and thereby increase sialylation of recombinant EPO and other glycoproteins. This study establishes 1,3,4‐ O ‐Bu 3 ManNAc as a novel chemical supplement to improve glycoprotein quality and sialylation levels at concentrations orders of magnitude lower than alternative approaches. Biotechnol. Bioeng. 2017;114: 1899–1902. © 2017 Wiley Periodicals, Inc." @default.
• W2603910151 created "2017-04-07" @default.
• W2603910151 creator A5014510444 @default.
• W2603910151 creator A5018117340 @default.
• W2603910151 creator A5029892873 @default.
• W2603910151 creator A5038164189 @default.
• W2603910151 creator A5046060166 @default.
• W2603910151 creator A5062493663 @default.
• W2603910151 creator A5067156333 @default.
• W2603910151 creator A5073838880 @default.
• W2603910151 date "2017-05-08" @default.
• W2603910151 modified "2023-10-18" @default.
• W2603910151 title "A novel sugar analog enhances sialic acid production and biotherapeutic sialylation in CHO cells" @default.
• W2603910151 cites W1589482850 @default.
• W2603910151 cites W1886156337 @default.
• W2603910151 cites W2006994535 @default.
• W2603910151 cites W2010782200 @default.
• W2603910151 cites W2018371082 @default.
• W2603910151 cites W2026298213 @default.
• W2603910151 cites W2027165192 @default.
• W2603910151 cites W2034450975 @default.
• W2603910151 cites W2037400245 @default.
• W2603910151 cites W2039083746 @default.
• W2603910151 cites W2047477273 @default.
• W2603910151 cites W2079324365 @default.
• W2603910151 cites W2095050095 @default.
• W2603910151 cites W2116570566 @default.
• W2603910151 cites W2128015484 @default.
• W2603910151 cites W2162035907 @default.
• W2603910151 doi "https://doi.org/10.1002/bit.26291" @default.
• W2603910151 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28295160" @default.
• W2603910151 hasPublicationYear "2017" @default.
• W2603910151 type Work @default.
• W2603910151 sameAs 2603910151 @default.
• W2603910151 citedByCount "27" @default.
• W2603910151 countsByYear W26039101512018 @default.
• W2603910151 countsByYear W26039101512019 @default.
• W2603910151 countsByYear W26039101512020 @default.
• W2603910151 countsByYear W26039101512021 @default.
• W2603910151 countsByYear W26039101512022 @default.
• W2603910151 countsByYear W26039101512023 @default.
• W2603910151 crossrefType "journal-article" @default.
• W2603910151 hasAuthorship W2603910151A5014510444 @default.
• W2603910151 hasAuthorship W2603910151A5018117340 @default.
• W2603910151 hasAuthorship W2603910151A5029892873 @default.
• W2603910151 hasAuthorship W2603910151A5038164189 @default.
• W2603910151 hasAuthorship W2603910151A5046060166 @default.
• W2603910151 hasAuthorship W2603910151A5062493663 @default.
• W2603910151 hasAuthorship W2603910151A5067156333 @default.
• W2603910151 hasAuthorship W2603910151A5073838880 @default.
• W2603910151 hasConcept C104317684 @default.
• W2603910151 hasConcept C108625454 @default.
• W2603910151 hasConcept C13426554 @default.
• W2603910151 hasConcept C170493617 @default.
• W2603910151 hasConcept C175656101 @default.
• W2603910151 hasConcept C185592680 @default.
• W2603910151 hasConcept C2777313579 @default.
• W2603910151 hasConcept C2780115458 @default.
• W2603910151 hasConcept C2781302033 @default.
• W2603910151 hasConcept C40767141 @default.
• W2603910151 hasConcept C54355233 @default.
• W2603910151 hasConcept C55493867 @default.
• W2603910151 hasConcept C81885089 @default.
• W2603910151 hasConcept C86803240 @default.
• W2603910151 hasConceptScore W2603910151C104317684 @default.
• W2603910151 hasConceptScore W2603910151C108625454 @default.
• W2603910151 hasConceptScore W2603910151C13426554 @default.
• W2603910151 hasConceptScore W2603910151C170493617 @default.
• W2603910151 hasConceptScore W2603910151C175656101 @default.
• W2603910151 hasConceptScore W2603910151C185592680 @default.
• W2603910151 hasConceptScore W2603910151C2777313579 @default.
• W2603910151 hasConceptScore W2603910151C2780115458 @default.
• W2603910151 hasConceptScore W2603910151C2781302033 @default.
• W2603910151 hasConceptScore W2603910151C40767141 @default.
• W2603910151 hasConceptScore W2603910151C54355233 @default.
• W2603910151 hasConceptScore W2603910151C55493867 @default.
• W2603910151 hasConceptScore W2603910151C81885089 @default.
• W2603910151 hasConceptScore W2603910151C86803240 @default.
• W2603910151 hasFunder F4320306076 @default.
• W2603910151 hasIssue "8" @default.
• W2603910151 hasLocation W26039101511 @default.
• W2603910151 hasLocation W26039101512 @default.
• W2603910151 hasOpenAccess W2603910151 @default.
• W2603910151 hasPrimaryLocation W26039101511 @default.
• W2603910151 hasRelatedWork W1676407394 @default.
• W2603910151 hasRelatedWork W174527674 @default.
• W2603910151 hasRelatedWork W1987290897 @default.
• W2603910151 hasRelatedWork W2005442970 @default.
• W2603910151 hasRelatedWork W2023419685 @default.
• W2603910151 hasRelatedWork W2129568270 @default.
• W2603910151 hasRelatedWork W2138783448 @default.
• W2603910151 hasRelatedWork W2213049771 @default.
• W2603910151 hasRelatedWork W2559157712 @default.
• W2603910151 hasRelatedWork W993338644 @default.
• W2603910151 hasVolume "114" @default.
• W2603910151 isParatext "false" @default.
• W2603910151 isRetracted "false" @default.
• W2603910151 magId "2603910151" @default.

• next