FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2604003604> ?p ?o ?g. }

• W2604003604 abstract "Erythritol, a four-carbon polyol synthesized by microorganisms as an osmoprotectant, is a natural sweetener produced on an industrial scale for decades. Despite the fact that the yeast Yarrowia lipolytica has been reported since the 1970s as an erythritol producer, the metabolic pathway of this polyol has never been characterized. It was shown that erythritol synthesis in yeast occurs via the pentose phosphate pathway (PPP). The oleaginous yeast Y. lipolytica is a good host for converting inexpensive glycerol into a value-added product such as erythritol. Glycerol is a renewable feedstock which is produced on a large scale as a waste product by many branches of industry.In this study, we functionally overexpressed four genes involved in the pentose phosphate pathway (PPP): gene YALI0E06479g encoding transketolase (TKL1), gene YALI0F15587g encoding transaldolase (TAL1), gene YALI0E22649g encoding glucose-6-phosphate dehydrogenase (ZWF1), and gene YALI0B15598g encoding 6-phosphogluconate dehydrogenase (GND1). Here, we show that the crucial gene for erythritol synthesis in Y. lipolytica is transketolase. Overexpression of this gene results in a twofold improvement in erythritol synthesis during a shake-flask experiment (58 g/L). Moreover, overexpression of TKL1 allows for efficient production of erythritol independently from the supplied dissolved oxygen. Fermentation conducted in a 5-L bioreactor at low agitation results in almost 70% higher titer of erythritol over the control strain.This work presents the importance of the PPP in erythritol synthesis and the feasibility for economic production of erythritol from glycerol by the yeast Y. lipolytica." @default.
• W2604003604 created "2017-04-07" @default.
• W2604003604 creator A5058806064 @default.
• W2604003604 creator A5083437468 @default.
• W2604003604 creator A5088806155 @default.
• W2604003604 date "2017-03-24" @default.
• W2604003604 modified "2023-10-12" @default.
• W2604003604 title "Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica" @default.
• W2604003604 cites W1701939279 @default.
• W2604003604 cites W1972914539 @default.
• W2604003604 cites W1979357338 @default.
• W2604003604 cites W1985281425 @default.
• W2604003604 cites W2001498181 @default.
• W2604003604 cites W2019012243 @default.
• W2604003604 cites W2020328041 @default.
• W2604003604 cites W2025139063 @default.
• W2604003604 cites W2037135970 @default.
• W2604003604 cites W2038889160 @default.
• W2604003604 cites W2053941926 @default.
• W2604003604 cites W2056364696 @default.
• W2604003604 cites W2062872837 @default.
• W2604003604 cites W2065578179 @default.
• W2604003604 cites W2083482521 @default.
• W2604003604 cites W2092802992 @default.
• W2604003604 cites W2094553899 @default.
• W2604003604 cites W2108207483 @default.
• W2604003604 cites W2114570899 @default.
• W2604003604 cites W2131183871 @default.
• W2604003604 cites W2152420103 @default.
• W2604003604 cites W2253507117 @default.
• W2604003604 cites W2271286273 @default.
• W2604003604 cites W2308703207 @default.
• W2604003604 cites W2508782622 @default.
• W2604003604 cites W2513296612 @default.
• W2604003604 cites W4302764575 @default.
• W2604003604 doi "https://doi.org/10.1186/s13068-017-0772-6" @default.
• W2604003604 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5366165" @default.
• W2604003604 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28352301" @default.
• W2604003604 hasPublicationYear "2017" @default.
• W2604003604 type Work @default.
• W2604003604 sameAs 2604003604 @default.
• W2604003604 citedByCount "68" @default.
• W2604003604 countsByYear W26040036042017 @default.
• W2604003604 countsByYear W26040036042018 @default.
• W2604003604 countsByYear W26040036042019 @default.
• W2604003604 countsByYear W26040036042020 @default.
• W2604003604 countsByYear W26040036042021 @default.
• W2604003604 countsByYear W26040036042022 @default.
• W2604003604 countsByYear W26040036042023 @default.
• W2604003604 crossrefType "journal-article" @default.
• W2604003604 hasAuthorship W2604003604A5058806064 @default.
• W2604003604 hasAuthorship W2604003604A5083437468 @default.
• W2604003604 hasAuthorship W2604003604A5088806155 @default.
• W2604003604 hasBestOaLocation W26040036041 @default.
• W2604003604 hasConcept C100544194 @default.
• W2604003604 hasConcept C103432212 @default.
• W2604003604 hasConcept C178790620 @default.
• W2604003604 hasConcept C181199279 @default.
• W2604003604 hasConcept C185592680 @default.
• W2604003604 hasConcept C20251656 @default.
• W2604003604 hasConcept C2777576037 @default.
• W2604003604 hasConcept C2779222958 @default.
• W2604003604 hasConcept C2779429346 @default.
• W2604003604 hasConcept C2779490540 @default.
• W2604003604 hasConcept C2779578285 @default.
• W2604003604 hasConcept C2779626320 @default.
• W2604003604 hasConcept C2780261296 @default.
• W2604003604 hasConcept C2781171952 @default.
• W2604003604 hasConcept C3409486 @default.
• W2604003604 hasConcept C38706188 @default.
• W2604003604 hasConcept C55493867 @default.
• W2604003604 hasConcept C86803240 @default.
• W2604003604 hasConceptScore W2604003604C100544194 @default.
• W2604003604 hasConceptScore W2604003604C103432212 @default.
• W2604003604 hasConceptScore W2604003604C178790620 @default.
• W2604003604 hasConceptScore W2604003604C181199279 @default.
• W2604003604 hasConceptScore W2604003604C185592680 @default.
• W2604003604 hasConceptScore W2604003604C20251656 @default.
• W2604003604 hasConceptScore W2604003604C2777576037 @default.
• W2604003604 hasConceptScore W2604003604C2779222958 @default.
• W2604003604 hasConceptScore W2604003604C2779429346 @default.
• W2604003604 hasConceptScore W2604003604C2779490540 @default.
• W2604003604 hasConceptScore W2604003604C2779578285 @default.
• W2604003604 hasConceptScore W2604003604C2779626320 @default.
• W2604003604 hasConceptScore W2604003604C2780261296 @default.
• W2604003604 hasConceptScore W2604003604C2781171952 @default.
• W2604003604 hasConceptScore W2604003604C3409486 @default.
• W2604003604 hasConceptScore W2604003604C38706188 @default.
• W2604003604 hasConceptScore W2604003604C55493867 @default.
• W2604003604 hasConceptScore W2604003604C86803240 @default.
• W2604003604 hasFunder F4320335039 @default.
• W2604003604 hasIssue "1" @default.
• W2604003604 hasLocation W26040036041 @default.
• W2604003604 hasLocation W26040036042 @default.
• W2604003604 hasLocation W26040036043 @default.
• W2604003604 hasLocation W26040036044 @default.
• W2604003604 hasOpenAccess W2604003604 @default.
• W2604003604 hasPrimaryLocation W26040036041 @default.
• W2604003604 hasRelatedWork W1500576496 @default.
• W2604003604 hasRelatedWork W2025951852 @default.

• next