FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2604044959> ?p ?o ?g. }

• W2604044959 abstract "Human cytomegalovirus (HCMV) infection is a major cause of morbidity and mortality in immunocompromised individuals and a leading cause of birth defects after congenital infection. Herpesvirus cell tropism is determined by gH/gL glycoprotein complexes in the viral envelope. The gH/gL complexes mediate virus entry into host cells through binding to host cell receptors and promote fusion of viral envelopes with cellular membranes. HCMVencodes two alternative gH/gL complexes, gH/gL/gO and gH/gL/pUL (128, 130, 131a) which both shape the broad cell tropism of HCMV. However, their respective host cellreceptors have not been identified.HCMV shows a broad cell tropism and readily produces infectious supernatant virus in cell culture. HCMV lacking the gH/gL/gO complex hardly produces infectious virus and spreads mainly cell-associated. By studying the binding of HCMV particles and recombinant gO-IgG Fc fusion proteins to fibroblasts, platelet-derived growth factor receptor-α (PDGFRα) could be identified as an HCMV entry receptor recognized by the gH/gL/gO complex. It could be shown that only gH/gL/gO-positive HCMV infects cells in a PDGFRα-dependent manner: i)Soluble PDGFRα receptor and the natural ligand PDGF-BB could inhibit infection with wildtype virus, but not infection with gO-knockout mutants. ii) Overexpression of PDGFRα enhanced infection with wildtype virus, but not infection with gO-knockout virus. It could also be shown that infection of PDGFRα-positive cells is up to 98% driven by the gH/gL/gO complex. This might indicate that, PDGFRα expression levels secure effective infection offirst target cells. The PDGFRα - gH/gL/gO interaction may thus be an important target for the design of anti-HCMV vaccines or new anti-HCMV drugs to prevent primary infection.Infection of mice with murine cytomegalovirus (MCMV) has been extensively used as a model to study HCMV infection in vivo. It has been shown before that MCMV alsoexpresses a gH/gL/gO complex which, when knocked out, exhibits a phenotype in cell culture comparable to gO-knockout mutants of HCMV. Here, it could be shown that gOknockout mutants of MCMV and HCMV show similar shifts in infection capacities for different cell types. MCMV progenies derived from different cell types differ like HCMV progenies derived from different cell types in their cell tropism. This is very likely due to cell type-dependent incorporation of more or less gH/gL/gO complexes. This makes the MCMV infection of mice a good model to study the role of the gH/gL/gO complex in vivo." @default.
• W2604044959 created "2017-04-07" @default.
• W2604044959 creator A5063129195 @default.
• W2604044959 date "2017-02-22" @default.
• W2604044959 modified "2023-09-24" @default.
• W2604044959 title "The role of the cytomegalovirus glycoprotein complex gHgLgO in virus entry" @default.
• W2604044959 hasPublicationYear "2017" @default.
• W2604044959 type Work @default.
• W2604044959 sameAs 2604044959 @default.
• W2604044959 citedByCount "0" @default.
• W2604044959 crossrefType "dissertation" @default.
• W2604044959 hasAuthorship W2604044959A5063129195 @default.
• W2604044959 hasConcept C140704245 @default.
• W2604044959 hasConcept C159047783 @default.
• W2604044959 hasConcept C170493617 @default.
• W2604044959 hasConcept C2522874641 @default.
• W2604044959 hasConcept C2779820661 @default.
• W2604044959 hasConcept C33602714 @default.
• W2604044959 hasConcept C35693153 @default.
• W2604044959 hasConcept C54355233 @default.
• W2604044959 hasConcept C58475186 @default.
• W2604044959 hasConcept C86803240 @default.
• W2604044959 hasConcept C97380033 @default.
• W2604044959 hasConceptScore W2604044959C140704245 @default.
• W2604044959 hasConceptScore W2604044959C159047783 @default.
• W2604044959 hasConceptScore W2604044959C170493617 @default.
• W2604044959 hasConceptScore W2604044959C2522874641 @default.
• W2604044959 hasConceptScore W2604044959C2779820661 @default.
• W2604044959 hasConceptScore W2604044959C33602714 @default.
• W2604044959 hasConceptScore W2604044959C35693153 @default.
• W2604044959 hasConceptScore W2604044959C54355233 @default.
• W2604044959 hasConceptScore W2604044959C58475186 @default.
• W2604044959 hasConceptScore W2604044959C86803240 @default.
• W2604044959 hasConceptScore W2604044959C97380033 @default.
• W2604044959 hasLocation W26040449591 @default.
• W2604044959 hasOpenAccess W2604044959 @default.
• W2604044959 hasPrimaryLocation W26040449591 @default.
• W2604044959 hasRelatedWork W1644368017 @default.
• W2604044959 hasRelatedWork W1985504467 @default.
• W2604044959 hasRelatedWork W2036343954 @default.
• W2604044959 hasRelatedWork W2046070673 @default.
• W2604044959 hasRelatedWork W2049356309 @default.
• W2604044959 hasRelatedWork W2059719108 @default.
• W2604044959 hasRelatedWork W2062137476 @default.
• W2604044959 hasRelatedWork W2083212703 @default.
• W2604044959 hasRelatedWork W2099002154 @default.
• W2604044959 hasRelatedWork W2121749962 @default.
• W2604044959 hasRelatedWork W2133533422 @default.
• W2604044959 hasRelatedWork W2142545465 @default.
• W2604044959 hasRelatedWork W2148862526 @default.
• W2604044959 hasRelatedWork W2160021770 @default.
• W2604044959 hasRelatedWork W2618380098 @default.
• W2604044959 hasRelatedWork W2765821029 @default.
• W2604044959 hasRelatedWork W3004000435 @default.
• W2604044959 hasRelatedWork W3099287726 @default.
• W2604044959 hasRelatedWork W3167766680 @default.
• W2604044959 isParatext "false" @default.
• W2604044959 isRetracted "false" @default.
• W2604044959 magId "2604044959" @default.
• W2604044959 workType "dissertation" @default.