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• W2604122009 abstract "The complex biological roles of transition metals create a challenge for investigating how cells of the brain interact with each other in healthy functioning and disease states. When imaging metal ions in such cells, many factors of the cellular environment need to be considered, especially when using a wide range of imaging agents to enable reliable interpretation of images and results. In this chapter, we focus on the use of fluorescent sensors for metals in a neuronal cellular environment, beginning with a summary of available fluorophores currently in the market, as well as some suggestions toward finding more tailored sensors for specific questions. We then describe the features of fluorescent probes, and highlight the parameters most crucial to different types of biological investigations. Finally, we provide a detailed method to prepare, treat, and image cellular models that should be applicable to a diverse range of fluorescent sensors, with suggestions for methods to analyze results using image analysis software." @default.
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• W2604122009 date "2017-01-01" @default.
• W2604122009 modified "2023-09-26" @default.
• W2604122009 title "Fluorescent Probes for the Analysis of Labile Metals in Brain Cells" @default.
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• W2604122009 doi "https://doi.org/10.1007/978-1-4939-6918-0_4" @default.
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