Matches in SemOpenAlex for { <https://semopenalex.org/work/W2604438536> ?p ?o ?g. }
- W2604438536 endingPage "5071" @default.
- W2604438536 startingPage "5066" @default.
- W2604438536 abstract "We here present a conceptually novel reaction-based ELISA principle (ReactELISA) for quantitation of the carbon nucleophilic lipid metabolite acetoacetate. Key to the assay is the utilization of a highly chemoselective Friedländer reaction that captures and simultaneously stabilizes the nucleophilic metabolite directly in the biological matrix. By developing a bifunctional biotinylated capture probe, the Friedländer-acetoacetate adduct can be trapped and purified directly in streptavidin coated wells. Finally, we outline the selection and refinement of a highly selective recombinant antibody for specific adduct quantitation. The setup is very robust and, as we demonstrate via miniaturization for microplate format, amenable for screening of compounds or interventions that alter lipid metabolism in liver cell cultures. The assay-principle should be extendable to quantitation of other nucleophilic or electrophilic and perhaps even more reactive metabolites provided suitable capture probes and antibodies." @default.
- W2604438536 created "2017-04-14" @default.
- W2604438536 creator A5001772428 @default.
- W2604438536 creator A5002597093 @default.
- W2604438536 creator A5012032867 @default.
- W2604438536 creator A5025456730 @default.
- W2604438536 creator A5030044825 @default.
- W2604438536 creator A5059282439 @default.
- W2604438536 creator A5071381146 @default.
- W2604438536 creator A5085417453 @default.
- W2604438536 date "2017-04-17" @default.
- W2604438536 modified "2023-10-09" @default.
- W2604438536 title "ReactELISA: Monitoring a Carbon Nucleophilic Metabolite by ELISA—a Study of Lipid Metabolism" @default.
- W2604438536 cites W1515531797 @default.
- W2604438536 cites W1548030969 @default.
- W2604438536 cites W1940319677 @default.
- W2604438536 cites W1974534288 @default.
- W2604438536 cites W1976112153 @default.
- W2604438536 cites W1980491680 @default.
- W2604438536 cites W1981638513 @default.
- W2604438536 cites W1997595831 @default.
- W2604438536 cites W2000167756 @default.
- W2604438536 cites W2014887370 @default.
- W2604438536 cites W2015233356 @default.
- W2604438536 cites W2016366885 @default.
- W2604438536 cites W2030497790 @default.
- W2604438536 cites W2032306298 @default.
- W2604438536 cites W2035182977 @default.
- W2604438536 cites W2035657933 @default.
- W2604438536 cites W2036551825 @default.
- W2604438536 cites W2047266987 @default.
- W2604438536 cites W2048376025 @default.
- W2604438536 cites W2060814375 @default.
- W2604438536 cites W2062364743 @default.
- W2604438536 cites W2062915487 @default.
- W2604438536 cites W2063028213 @default.
- W2604438536 cites W2063522459 @default.
- W2604438536 cites W2080959815 @default.
- W2604438536 cites W2082873025 @default.
- W2604438536 cites W2083741341 @default.
- W2604438536 cites W2087444227 @default.
- W2604438536 cites W2098343475 @default.
- W2604438536 cites W2100159014 @default.
- W2604438536 cites W2101130660 @default.
- W2604438536 cites W2120368353 @default.
- W2604438536 cites W2136503478 @default.
- W2604438536 cites W2137001034 @default.
- W2604438536 cites W2148685655 @default.
- W2604438536 cites W2153413087 @default.
- W2604438536 cites W2164527517 @default.
- W2604438536 cites W2190507182 @default.
- W2604438536 cites W2298455799 @default.
- W2604438536 cites W62898540 @default.
- W2604438536 doi "https://doi.org/10.1021/acs.analchem.7b00507" @default.
- W2604438536 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28376300" @default.
- W2604438536 hasPublicationYear "2017" @default.
- W2604438536 type Work @default.
- W2604438536 sameAs 2604438536 @default.
- W2604438536 citedByCount "8" @default.
- W2604438536 countsByYear W26044385362019 @default.
- W2604438536 countsByYear W26044385362020 @default.
- W2604438536 countsByYear W26044385362021 @default.
- W2604438536 countsByYear W26044385362023 @default.
- W2604438536 crossrefType "journal-article" @default.
- W2604438536 hasAuthorship W2604438536A5001772428 @default.
- W2604438536 hasAuthorship W2604438536A5002597093 @default.
- W2604438536 hasAuthorship W2604438536A5012032867 @default.
- W2604438536 hasAuthorship W2604438536A5025456730 @default.
- W2604438536 hasAuthorship W2604438536A5030044825 @default.
- W2604438536 hasAuthorship W2604438536A5059282439 @default.
- W2604438536 hasAuthorship W2604438536A5071381146 @default.
- W2604438536 hasAuthorship W2604438536A5085417453 @default.
- W2604438536 hasConcept C108204754 @default.
- W2604438536 hasConcept C159654299 @default.
- W2604438536 hasConcept C161790260 @default.
- W2604438536 hasConcept C178790620 @default.
- W2604438536 hasConcept C178907741 @default.
- W2604438536 hasConcept C185592680 @default.
- W2604438536 hasConcept C203014093 @default.
- W2604438536 hasConcept C204628709 @default.
- W2604438536 hasConcept C21951064 @default.
- W2604438536 hasConcept C2776190903 @default.
- W2604438536 hasConcept C2777477808 @default.
- W2604438536 hasConcept C2778204606 @default.
- W2604438536 hasConcept C2779909307 @default.
- W2604438536 hasConcept C43617362 @default.
- W2604438536 hasConcept C50027330 @default.
- W2604438536 hasConcept C542903549 @default.
- W2604438536 hasConcept C55493867 @default.
- W2604438536 hasConcept C62231903 @default.
- W2604438536 hasConcept C86803240 @default.
- W2604438536 hasConceptScore W2604438536C108204754 @default.
- W2604438536 hasConceptScore W2604438536C159654299 @default.
- W2604438536 hasConceptScore W2604438536C161790260 @default.
- W2604438536 hasConceptScore W2604438536C178790620 @default.
- W2604438536 hasConceptScore W2604438536C178907741 @default.
- W2604438536 hasConceptScore W2604438536C185592680 @default.
- W2604438536 hasConceptScore W2604438536C203014093 @default.