FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2604455965> ?p ?o ?g. }

• W2604455965 endingPage "1864" @default.
• W2604455965 startingPage "1857" @default.
• W2604455965 abstract "Methicillin-resistant Staphylococcus aureus (MRSA) is a clinically significant pathogen that is resistant to a wide variety of antibiotics and responsible for a large number of nosocomial infections worldwide. The Agency for Healthcare Research and Quality and the Centers for Disease Control and Prevention recently recommended the adoption of universal mupirocin-chlorhexidine decolonization of all admitted intensive care unit patients rather than MRSA screening with targeted treatments, which raises a serious concern about the selection of resistance to mupirocin and chlorhexidine in strains of staphylococci. Thus, a simple, rapid, and reliable approach is paramount in monitoring the prevalence of resistance to these agents. We developed a simple multiplex PCR assay capable of screening Staphylococcus isolates for the presence of antiseptic resistance genes for chlorhexidine and quaternary ammonium compounds, as well as mupirocin and methicillin resistance genes, while simultaneously discriminating S. aureus from coagulase-negative staphylococci (CoNS). The assay incorporates 7 PCR targets, including the Staphylococcus 16S rRNA gene (specifically detecting Staphylococcus spp.), nuc (distinguishing S. aureus from CoNS), mecA (distinguishing MRSA from methicillin-susceptible S. aureus), mupA and mupB (identifying high-level mupirocin resistance), and qac and smr (identifying chlorhexidine and quaternary ammonium resistance). Our assay demonstrated 100% sensitivity, specificity, and accuracy in a total of 23 variant antiseptic- and/or antibiotic-resistant control strains. Further validation of our assay using 378 randomly selected and previously well-characterized local clinical isolates confirmed its feasibility and practicality. This may prove to be a useful tool for multidrug-resistant Staphylococcus monitoring in clinical laboratories, particularly in the wake of increased chlorhexidine and mupirocin treatments." @default.
• W2604455965 created "2017-04-14" @default.
• W2604455965 creator A5009969992 @default.
• W2604455965 creator A5057810321 @default.
• W2604455965 creator A5059368424 @default.
• W2604455965 creator A5089536235 @default.
• W2604455965 date "2017-06-01" @default.
• W2604455965 modified "2023-10-14" @default.
• W2604455965 title "Novel Multiplex PCR Assay for Detection of Chlorhexidine-Quaternary Ammonium, Mupirocin, and Methicillin Resistance Genes, with Simultaneous Discrimination of Staphylococcus aureus from Coagulase-Negative Staphylococci" @default.
• W2604455965 cites W1927502485 @default.
• W2604455965 cites W1963979170 @default.
• W2604455965 cites W1970884855 @default.
• W2604455965 cites W1975788373 @default.
• W2604455965 cites W1980569249 @default.
• W2604455965 cites W1980929211 @default.
• W2604455965 cites W1986801001 @default.
• W2604455965 cites W2004710466 @default.
• W2604455965 cites W2057014803 @default.
• W2604455965 cites W2062103917 @default.
• W2604455965 cites W2064016965 @default.
• W2604455965 cites W2082836578 @default.
• W2604455965 cites W2087865186 @default.
• W2604455965 cites W2088409954 @default.
• W2604455965 cites W2091404988 @default.
• W2604455965 cites W2103417372 @default.
• W2604455965 cites W2106659357 @default.
• W2604455965 cites W2115650349 @default.
• W2604455965 cites W2117424518 @default.
• W2604455965 cites W2119606443 @default.
• W2604455965 cites W2120254667 @default.
• W2604455965 cites W2121256723 @default.
• W2604455965 cites W2121577018 @default.
• W2604455965 cites W2129100984 @default.
• W2604455965 cites W2132380597 @default.
• W2604455965 cites W2134469459 @default.
• W2604455965 cites W2135956204 @default.
• W2604455965 cites W2140025834 @default.
• W2604455965 cites W2159022937 @default.
• W2604455965 cites W2166776540 @default.
• W2604455965 cites W2337992310 @default.
• W2604455965 cites W2406824113 @default.
• W2604455965 cites W4230543984 @default.
• W2604455965 cites W4243166839 @default.
• W2604455965 doi "https://doi.org/10.1128/jcm.02488-16" @default.
• W2604455965 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5442542" @default.
• W2604455965 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28381601" @default.
• W2604455965 hasPublicationYear "2017" @default.
• W2604455965 type Work @default.
• W2604455965 sameAs 2604455965 @default.
• W2604455965 citedByCount "16" @default.
• W2604455965 countsByYear W26044559652017 @default.
• W2604455965 countsByYear W26044559652018 @default.
• W2604455965 countsByYear W26044559652019 @default.
• W2604455965 countsByYear W26044559652020 @default.
• W2604455965 countsByYear W26044559652021 @default.
• W2604455965 countsByYear W26044559652022 @default.
• W2604455965 countsByYear W26044559652023 @default.
• W2604455965 crossrefType "journal-article" @default.
• W2604455965 hasAuthorship W2604455965A5009969992 @default.
• W2604455965 hasAuthorship W2604455965A5057810321 @default.
• W2604455965 hasAuthorship W2604455965A5059368424 @default.
• W2604455965 hasAuthorship W2604455965A5089536235 @default.
• W2604455965 hasBestOaLocation W26044559651 @default.
• W2604455965 hasConcept C142724271 @default.
• W2604455965 hasConcept C199343813 @default.
• W2604455965 hasConcept C2777052132 @default.
• W2604455965 hasConcept C2777084678 @default.
• W2604455965 hasConcept C2777334864 @default.
• W2604455965 hasConcept C2778246068 @default.
• W2604455965 hasConcept C2779288629 @default.
• W2604455965 hasConcept C2779489039 @default.
• W2604455965 hasConcept C2781460226 @default.
• W2604455965 hasConcept C501593827 @default.
• W2604455965 hasConcept C523546767 @default.
• W2604455965 hasConcept C54355233 @default.
• W2604455965 hasConcept C71924100 @default.
• W2604455965 hasConcept C8408860 @default.
• W2604455965 hasConcept C86803240 @default.
• W2604455965 hasConcept C89423630 @default.
• W2604455965 hasConcept C94665300 @default.
• W2604455965 hasConceptScore W2604455965C142724271 @default.
• W2604455965 hasConceptScore W2604455965C199343813 @default.
• W2604455965 hasConceptScore W2604455965C2777052132 @default.
• W2604455965 hasConceptScore W2604455965C2777084678 @default.
• W2604455965 hasConceptScore W2604455965C2777334864 @default.
• W2604455965 hasConceptScore W2604455965C2778246068 @default.
• W2604455965 hasConceptScore W2604455965C2779288629 @default.
• W2604455965 hasConceptScore W2604455965C2779489039 @default.
• W2604455965 hasConceptScore W2604455965C2781460226 @default.
• W2604455965 hasConceptScore W2604455965C501593827 @default.
• W2604455965 hasConceptScore W2604455965C523546767 @default.
• W2604455965 hasConceptScore W2604455965C54355233 @default.
• W2604455965 hasConceptScore W2604455965C71924100 @default.
• W2604455965 hasConceptScore W2604455965C8408860 @default.
• W2604455965 hasConceptScore W2604455965C86803240 @default.
• W2604455965 hasConceptScore W2604455965C89423630 @default.
• W2604455965 hasConceptScore W2604455965C94665300 @default.
• W2604455965 hasFunder F4320309907 @default.

• next