FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2604632264> ?p ?o ?g. }

• W2604632264 endingPage "165" @default.
• W2604632264 startingPage "154" @default.
• W2604632264 abstract "Rationale: Genetic therapy using modified mRNA for specific therapeutic protein expression for disease treatment and vaccination represents a new field of therapeutic and diagnostic medicine. Non-viral vectors transfection using biocompatible nanoliposomes enables safe and efficient delivery of therapeutic mRNA. Objective: Generation of non-toxic, cell-compatible cationic nanoliposomes as nanotheranostic agents to successfully deliver therapeutic mRNA. Methods and results: Cationic nanoliposomes (DC-Cholesterol/DOPE) were generated as transfection vehicles for either eGFP mRNA or the therapeutic anti-inflammatory, CD39 mRNA. We observed no toxicity using these nanoplexes and noted high cell viability after transfection. Nanoplexes for the transfection of eGFP mRNA showed an increase in fluorescence signals on microscopy as compared to the mRNA control after 24 hours in Chinese hamster ovary (CHO) cells (14.29 ± 5.30 vs. 1.49 ± 0.54; mean ± SD respectively; p<0.001) and flow cytometry (57.29 ± 14.59 vs 1.83 ± 0.34; % mean ± SD; p<0.001). Nanoplexes for the transfection of CD39 mRNA showed increased CD39 expression in flow cytometry (45.64 ± 15.3 vs. 3.94 ± 0.45; % mean ± SD; p<0.001) as compared to the mRNA control after 24 hours using CHO cells. We also demonstrated efficient transfection across several cell lines (CHO, HEK293, and A549), as well as long-term protein expression (120 h and 168 h) using these nanoplexes. Conclusions: We have developed and tested non-toxic, safe, and efficient nanoliposome preparations for the delivery of therapeutic mRNA that hold promise for novel therapies in diseases such as inflammatory and cardiovascular diseases, as well as cancer. We have also demonstrated that this approach provides a reliable technology to deliver CD39 mRNA as an anti-inflammatory therapeutic for future nanotheranostics approaches." @default.
• W2604632264 created "2017-04-14" @default.
• W2604632264 creator A5003584263 @default.
• W2604632264 creator A5008496267 @default.
• W2604632264 creator A5023142640 @default.
• W2604632264 creator A5023919458 @default.
• W2604632264 creator A5049936296 @default.
• W2604632264 creator A5076573247 @default.
• W2604632264 date "2017-01-01" @default.
• W2604632264 modified "2023-10-18" @default.
• W2604632264 title "Nanoliposomes for Safe and Efficient Therapeutic mRNA Delivery: A Step Toward Nanotheranostics in Inflammatory and Cardiovascular Diseases as well as Cancer" @default.
• W2604632264 cites W1606886936 @default.
• W2604632264 cites W1893534297 @default.
• W2604632264 cites W1963706689 @default.
• W2604632264 cites W1965316405 @default.
• W2604632264 cites W1972510968 @default.
• W2604632264 cites W1972531115 @default.
• W2604632264 cites W1982151061 @default.
• W2604632264 cites W1986368285 @default.
• W2604632264 cites W1988620094 @default.
• W2604632264 cites W1994674548 @default.
• W2604632264 cites W1995717711 @default.
• W2604632264 cites W2000130544 @default.
• W2604632264 cites W2021538200 @default.
• W2604632264 cites W2025605461 @default.
• W2604632264 cites W2026083914 @default.
• W2604632264 cites W2031785518 @default.
• W2604632264 cites W2032517146 @default.
• W2604632264 cites W2051091654 @default.
• W2604632264 cites W2053359824 @default.
• W2604632264 cites W2053447722 @default.
• W2604632264 cites W2055351480 @default.
• W2604632264 cites W2055676760 @default.
• W2604632264 cites W2057395225 @default.
• W2604632264 cites W2061340549 @default.
• W2604632264 cites W2067087090 @default.
• W2604632264 cites W2072405413 @default.
• W2604632264 cites W2077112895 @default.
• W2604632264 cites W2081712965 @default.
• W2604632264 cites W2083200530 @default.
• W2604632264 cites W2084263949 @default.
• W2604632264 cites W2089477917 @default.
• W2604632264 cites W2092386113 @default.
• W2604632264 cites W2092509331 @default.
• W2604632264 cites W2097603366 @default.
• W2604632264 cites W2108209050 @default.
• W2604632264 cites W2127430173 @default.
• W2604632264 cites W2130400901 @default.
• W2604632264 cites W2142018422 @default.
• W2604632264 cites W2144985844 @default.
• W2604632264 cites W2150083988 @default.
• W2604632264 cites W2255276698 @default.
• W2604632264 cites W2318091769 @default.
• W2604632264 cites W2378193834 @default.
• W2604632264 cites W2438888815 @default.
• W2604632264 cites W2567120106 @default.
• W2604632264 doi "https://doi.org/10.7150/ntno.19449" @default.
• W2604632264 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5646717" @default.
• W2604632264 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29071184" @default.
• W2604632264 hasPublicationYear "2017" @default.
• W2604632264 type Work @default.
• W2604632264 sameAs 2604632264 @default.
• W2604632264 citedByCount "18" @default.
• W2604632264 countsByYear W26046322642018 @default.
• W2604632264 countsByYear W26046322642019 @default.
• W2604632264 countsByYear W26046322642020 @default.
• W2604632264 countsByYear W26046322642021 @default.
• W2604632264 countsByYear W26046322642022 @default.
• W2604632264 countsByYear W26046322642023 @default.
• W2604632264 crossrefType "journal-article" @default.
• W2604632264 hasAuthorship W2604632264A5003584263 @default.
• W2604632264 hasAuthorship W2604632264A5008496267 @default.
• W2604632264 hasAuthorship W2604632264A5023142640 @default.
• W2604632264 hasAuthorship W2604632264A5023919458 @default.
• W2604632264 hasAuthorship W2604632264A5049936296 @default.
• W2604632264 hasAuthorship W2604632264A5076573247 @default.
• W2604632264 hasBestOaLocation W26046322641 @default.
• W2604632264 hasConcept C104317684 @default.
• W2604632264 hasConcept C105580179 @default.
• W2604632264 hasConcept C111599444 @default.
• W2604632264 hasConcept C135983454 @default.
• W2604632264 hasConcept C1491633281 @default.
• W2604632264 hasConcept C153911025 @default.
• W2604632264 hasConcept C175656101 @default.
• W2604632264 hasConcept C502942594 @default.
• W2604632264 hasConcept C53227056 @default.
• W2604632264 hasConcept C54009773 @default.
• W2604632264 hasConcept C54355233 @default.
• W2604632264 hasConcept C553184892 @default.
• W2604632264 hasConcept C55493867 @default.
• W2604632264 hasConcept C71924100 @default.
• W2604632264 hasConcept C81885089 @default.
• W2604632264 hasConcept C86803240 @default.
• W2604632264 hasConceptScore W2604632264C104317684 @default.
• W2604632264 hasConceptScore W2604632264C105580179 @default.
• W2604632264 hasConceptScore W2604632264C111599444 @default.
• W2604632264 hasConceptScore W2604632264C135983454 @default.
• W2604632264 hasConceptScore W2604632264C1491633281 @default.

• next