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- W2604804017 abstract "Invertase immobilized on magnetic diatomaceous earth nanoparticles (mDE-APTES-invertase) with high sucrolytic activity was obtained by an easy and low-cost method. An experimental design was carried out to investigate the best immobilization conditions and it allowed obtaining an immobilized derivative with a residual specific activity equal to 92.5%. Then, a second experimental design selected the mDE-APTES-invertase with higher specific activity in relation to other derivatives reported in the literature (2.42-fold). Thermal and storage stability for immobilized invertase were found to be 35 °C for 60 min (85% retained activity) and 120 days storage period (80% retained activity), respectively. Besides, a residual activity higher than 60% and 50% were observed for mDE-APTES-invertase after reuse in short and long term, respectively. Given the simple and efficient method to obtain an immobilized derivative with high activity, the mDE nanoparticles appear to be a promising matrix for invertase immobilization as well as for other biomolecules." @default.
- W2604804017 created "2017-04-14" @default.
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- W2604804017 date "2017-03-01" @default.
- W2604804017 modified "2023-10-18" @default.
- W2604804017 title "High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles" @default.
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- W2604804017 doi "https://doi.org/10.1016/j.btre.2017.03.001" @default.
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