FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2605202107> ?p ?o ?g. }

• W2605202107 endingPage "e0174990" @default.
• W2605202107 startingPage "e0174990" @default.
• W2605202107 abstract "Although several in vitro and ex vivo evidence support the existence of lactate exchange between astrocytes and neurons, a direct demonstration in vivo is still lacking. In the present study, a lentiviral vector carrying a short hairpin RNA (shRNA) was used to downregulate the expression of the monocarboxylate transporter type 2 (MCT2) in neurons of the rat somatosensory cortex (called S1BF) by ~ 25%. After one hour of whisker stimulation, HRMAS 1H-NMR spectroscopy analysis of S1BF perchloric acid extracts showed that while an increase in lactate content is observed in both uninjected and shRNA-control injected extracts, such an effect was abrogated in shMCT2 injected rats. A 13C-incorporation analysis following [1-13C]glucose infusion during the stimulation confirmed that the elevated lactate observed during activation originates from newly synthesized [3-13C]lactate, with blood-derived [1-13C]glucose being the precursor. Moreover, the analysis of the 13C-labeling of glutamate in position C3 and C4 indicates that upon activation, there is an increase in TCA cycle velocity for control rats while a decrease is observed for MCT2 knockdown animals. Using in vivo localized 1H-NMR spectroscopy, an increase in lactate levels is observed in the S1BF area upon whisker stimulation for shRNA-control injected rats but not for MCT2 knockdown animals. Finally, while a robust BOLD fMRI response was evidenced in control rats, it was absent in MCT2 knockdown rats. These data not only demonstrate that glucose-derived lactate is locally produced following neuronal activation but also suggest that its use by neurons via MCT2 is probably essential to maintain synaptic activity within the barrel cortex." @default.
• W2605202107 created "2017-04-14" @default.
• W2605202107 creator A5003282136 @default.
• W2605202107 creator A5013839554 @default.
• W2605202107 creator A5022456505 @default.
• W2605202107 creator A5029013194 @default.
• W2605202107 creator A5030125855 @default.
• W2605202107 creator A5045897447 @default.
• W2605202107 creator A5068359933 @default.
• W2605202107 creator A5071844167 @default.
• W2605202107 creator A5087753311 @default.
• W2605202107 date "2017-04-07" @default.
• W2605202107 modified "2023-09-24" @default.
• W2605202107 title "A neuronal MCT2 knockdown in the rat somatosensory cortex reduces both the NMR lactate signal and the BOLD response during whisker stimulation" @default.
• W2605202107 cites W1517853766 @default.
• W2605202107 cites W1522786769 @default.
• W2605202107 cites W1775749144 @default.
• W2605202107 cites W1888179590 @default.
• W2605202107 cites W1965279482 @default.
• W2605202107 cites W1965601223 @default.
• W2605202107 cites W1967009686 @default.
• W2605202107 cites W1969454688 @default.
• W2605202107 cites W1971082249 @default.
• W2605202107 cites W1971199626 @default.
• W2605202107 cites W1972616149 @default.
• W2605202107 cites W1976517061 @default.
• W2605202107 cites W1978262170 @default.
• W2605202107 cites W1982422255 @default.
• W2605202107 cites W1983924090 @default.
• W2605202107 cites W1984089779 @default.
• W2605202107 cites W1984496822 @default.
• W2605202107 cites W1987146019 @default.
• W2605202107 cites W1990588470 @default.
• W2605202107 cites W1993303421 @default.
• W2605202107 cites W2001725826 @default.
• W2605202107 cites W2002568228 @default.
• W2605202107 cites W2005925597 @default.
• W2605202107 cites W2009095360 @default.
• W2605202107 cites W2009966509 @default.
• W2605202107 cites W2014988592 @default.
• W2605202107 cites W2015200656 @default.
• W2605202107 cites W2015741711 @default.
• W2605202107 cites W2017160766 @default.
• W2605202107 cites W2018314891 @default.
• W2605202107 cites W2030431065 @default.
• W2605202107 cites W2053268711 @default.
• W2605202107 cites W2057415382 @default.
• W2605202107 cites W2059028489 @default.
• W2605202107 cites W2059670143 @default.
• W2605202107 cites W2062927130 @default.
• W2605202107 cites W2064898829 @default.
• W2605202107 cites W2067540207 @default.
• W2605202107 cites W2069987358 @default.
• W2605202107 cites W2072203762 @default.
• W2605202107 cites W2072278357 @default.
• W2605202107 cites W2081918955 @default.
• W2605202107 cites W2092635505 @default.
• W2605202107 cites W2092651201 @default.
• W2605202107 cites W2100212553 @default.
• W2605202107 cites W2107450931 @default.
• W2605202107 cites W2126370598 @default.
• W2605202107 cites W2126525449 @default.
• W2605202107 cites W2130190854 @default.
• W2605202107 cites W2148611607 @default.
• W2605202107 cites W2152068171 @default.
• W2605202107 cites W2155957566 @default.
• W2605202107 cites W2202641067 @default.
• W2605202107 cites W2581215498 @default.
• W2605202107 doi "https://doi.org/10.1371/journal.pone.0174990" @default.
• W2605202107 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5384673" @default.
• W2605202107 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28388627" @default.
• W2605202107 hasPublicationYear "2017" @default.
• W2605202107 type Work @default.
• W2605202107 sameAs 2605202107 @default.
• W2605202107 citedByCount "37" @default.
• W2605202107 countsByYear W26052021072017 @default.
• W2605202107 countsByYear W26052021072018 @default.
• W2605202107 countsByYear W26052021072019 @default.
• W2605202107 countsByYear W26052021072020 @default.
• W2605202107 countsByYear W26052021072021 @default.
• W2605202107 countsByYear W26052021072022 @default.
• W2605202107 countsByYear W26052021072023 @default.
• W2605202107 crossrefType "journal-article" @default.
• W2605202107 hasAuthorship W2605202107A5003282136 @default.
• W2605202107 hasAuthorship W2605202107A5013839554 @default.
• W2605202107 hasAuthorship W2605202107A5022456505 @default.
• W2605202107 hasAuthorship W2605202107A5029013194 @default.
• W2605202107 hasAuthorship W2605202107A5030125855 @default.
• W2605202107 hasAuthorship W2605202107A5045897447 @default.
• W2605202107 hasAuthorship W2605202107A5068359933 @default.
• W2605202107 hasAuthorship W2605202107A5071844167 @default.
• W2605202107 hasAuthorship W2605202107A5087753311 @default.
• W2605202107 hasBestOaLocation W26052021071 @default.
• W2605202107 hasConcept C126322002 @default.
• W2605202107 hasConcept C134018914 @default.
• W2605202107 hasConcept C150903083 @default.
• W2605202107 hasConcept C173396325 @default.
• W2605202107 hasConcept C185592680 @default.

• next