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• W2605235148 abstract "Accurate diagnosis of diarrhoea caused by enteric parasites requires rapid, cost-effective, scalable, high-throughput, reproducible, and sensitive detection methods. Mixed infections are common, requiring clinicians and the detection methods they employ to screen for multiple pathogens. Current detection methods are labour-intensive, time-consuming, lack sensitivity and specificity, and fail to detect mixed infections. Gastrointestinal panel (GIP) assays based on multiplex PCR afford the opportunity to detect mixed infections and adhere to the requirements for diarrhoeal disease diagnosis. Costs associated with GIP assays are still unaffordable for laboratories, particularly in developing countries. More cost-effective, field-deployable technologies are required. Enteric parasites are major contributors to the global diarrhoeal disease load, infecting >67.2 million people. Their prevalence and clinical impact, however, are underestimated due to lack of adequate detection, which is largely still based on microscopy, particularly in developing countries. New commercially available enteric panel assays, which detect parasites (as well as bacteria and/or viruses) using multiplex PCR, offer enhanced sensitivity and specificity as well as the ability to detect mixed infections, and will play an important role in epidemiological surveillance and outbreak investigations. A major limitation of these technologies, however, particularly for developing countries, is the costs involved. Emerging technologies for low-resource, point-of-care (POC) settings have the potential to dramatically improve the cost and accuracy of enteric parasite detection in the future. Enteric parasites are major contributors to the global diarrhoeal disease load, infecting >67.2 million people. Their prevalence and clinical impact, however, are underestimated due to lack of adequate detection, which is largely still based on microscopy, particularly in developing countries. New commercially available enteric panel assays, which detect parasites (as well as bacteria and/or viruses) using multiplex PCR, offer enhanced sensitivity and specificity as well as the ability to detect mixed infections, and will play an important role in epidemiological surveillance and outbreak investigations. A major limitation of these technologies, however, particularly for developing countries, is the costs involved. Emerging technologies for low-resource, point-of-care (POC) settings have the potential to dramatically improve the cost and accuracy of enteric parasite detection in the future. Una Ryan: On the Importance of Clean Potable Water et al.Trends in ParasitologyApril 19, 2017In BriefUna Ryan is a Professor in Biochemistry at Murdoch University in Australia. Her research focuses, in part, on the molecular detection of diarrhea-causing parasites, such as Cryptosporidium and Giardia. Diarrheal disease can be prevented. However, it is the second leading cause of death in children under 5 years of age in developing countries. Children living in poor or remote communities, without access to safe drinking water and adequate sanitation and hygiene are most at risk. The World Health Organization (WHO) aims to end preventable deaths caused by diarrheal disease by 2025 by, among other initiatives, increasing access to safe drinking water and sanitation in developing countries. Full-Text PDF" @default.
• W2605235148 created "2017-04-14" @default.
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• W2605235148 date "2017-07-01" @default.
• W2605235148 modified "2023-10-14" @default.
• W2605235148 title "New Technologies for Detection of Enteric Parasites" @default.
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