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- W2605332712 abstract "Nucleic-acid-based matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) applications for the detection and identification of viruses use specific or broad-range polymerase chain reaction (PCR) primers in combination with different post-PCR biochemistries. The most widely used biochemistry and variations thereof, a PCR/primer extension assay has been applied to the detection and genotyping of RNA and DNA viruses with a multiplexing level and throughput that support large-scale epidemiological research studies and might improve diagnosis of infections and co-infections in clinical applications. The study demonstrates the importance of genetic analysis and informative molecular markers for rapid and high-throughput epidemiological studies of pathogen transmission. In addition to the detection of viruses, PCR/primer extension assays on MALDI-TOF MS enable the characterization of viruses by genotyping. PCR/primer extension assays have successfully been applied to genotype Hepatitis B virus (HBV) and to detect up to 60 single-nucleotide drug resistance mutations in the HBV polymerase gene." @default.
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- W2605332712 date "2017-04-07" @default.
- W2605332712 modified "2023-10-16" @default.
- W2605332712 title "Microbial DNA Analysis by MALDI-TOF Mass Spectrometry" @default.
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- W2605332712 doi "https://doi.org/10.1002/9781118960226.ch8" @default.
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