FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2605548152> ?p ?o ?g. }

• W2605548152 endingPage "9582" @default.
• W2605548152 startingPage "9567" @default.
• W2605548152 abstract "DNA sequences that are guanine-rich have received considerable attention because of their potential to fold into a secondary, four-stranded DNA structure termed G-quadruplex (G4), which has been implicated in genomic instability and some human diseases. We have previously identified positive coactivator of transcription (PC4), a single-stranded DNA (ssDNA)-binding protein, as a novel G4 interactor. Here, to expand on these previous observations, we biochemically and biophysically characterized the interaction between PC4 and G4DNA. PC4 can bind alternative G4DNA topologies with a low nanomolar Kd value of ∼2 nm, similar to that observed for ssDNA. In consideration of the different structural features between G4DNA and ssDNA, these binding data indicated that PC4 can interact with G4DNA in a manner distinct from ssDNA. The stoichiometry of the PC4-G4 complex was 1:1 for PC4 dimer:G4 substrate. PC4 did not enhance the rate of folding of G4DNA, and formation of the PC4-G4DNA complex did not result in unfolding of the G4DNA structure. We assembled a G4DNA structure flanked by duplex DNA. We find that PC4 can interact with this G4DNA, as well as the complementary C-rich strand. Molecular docking simulations and DNA footprinting experiments suggest a model where a PC4 dimer accommodates the DNA with one monomer on the G4 strand and the second monomer bound to the C-rich strand. Collectively, these data provide a novel mode of PC4 binding to a DNA secondary structure that remains within the framework of the model for binding to ssDNA. Additionally, consideration of the PC4-G4DNA interaction could provide insight into the biological functions of PC4, which remain incompletely understood." @default.
• W2605548152 created "2017-04-28" @default.
• W2605548152 creator A5008476359 @default.
• W2605548152 creator A5023637844 @default.
• W2605548152 creator A5033876035 @default.
• W2605548152 creator A5040884216 @default.
• W2605548152 creator A5052449333 @default.
• W2605548152 date "2017-07-01" @default.
• W2605548152 modified "2023-10-12" @default.
• W2605548152 title "A biochemical and biophysical model of G-quadruplex DNA recognition by positive coactivator of transcription 4" @default.
• W2605548152 cites W1914649288 @default.
• W2605548152 cites W1929664900 @default.
• W2605548152 cites W1960314762 @default.
• W2605548152 cites W1964068975 @default.
• W2605548152 cites W1972987821 @default.
• W2605548152 cites W1976933659 @default.
• W2605548152 cites W1979198416 @default.
• W2605548152 cites W1981735048 @default.
• W2605548152 cites W1988640412 @default.
• W2605548152 cites W1988708102 @default.
• W2605548152 cites W1995141673 @default.
• W2605548152 cites W1995472790 @default.
• W2605548152 cites W1996101871 @default.
• W2605548152 cites W1999472367 @default.
• W2605548152 cites W2008871447 @default.
• W2605548152 cites W2013646237 @default.
• W2605548152 cites W2017226419 @default.
• W2605548152 cites W2017983775 @default.
• W2605548152 cites W2025485946 @default.
• W2605548152 cites W2029972314 @default.
• W2605548152 cites W2033668791 @default.
• W2605548152 cites W2034103505 @default.
• W2605548152 cites W2037020472 @default.
• W2605548152 cites W2038929178 @default.
• W2605548152 cites W2039347157 @default.
• W2605548152 cites W2043198085 @default.
• W2605548152 cites W2044508856 @default.
• W2605548152 cites W2045868610 @default.
• W2605548152 cites W2049093399 @default.
• W2605548152 cites W2055681204 @default.
• W2605548152 cites W2055988101 @default.
• W2605548152 cites W2058384455 @default.
• W2605548152 cites W2059306569 @default.
• W2605548152 cites W2059540845 @default.
• W2605548152 cites W2070883648 @default.
• W2605548152 cites W2077605689 @default.
• W2605548152 cites W2078024052 @default.
• W2605548152 cites W2079470630 @default.
• W2605548152 cites W2080464053 @default.
• W2605548152 cites W2084052406 @default.
• W2605548152 cites W2086499080 @default.
• W2605548152 cites W2087123043 @default.
• W2605548152 cites W2088669429 @default.
• W2605548152 cites W2093320042 @default.
• W2605548152 cites W2093356995 @default.
• W2605548152 cites W2093816990 @default.
• W2605548152 cites W2094340255 @default.
• W2605548152 cites W2100013366 @default.
• W2605548152 cites W2109298991 @default.
• W2605548152 cites W2110023342 @default.
• W2605548152 cites W2116609429 @default.
• W2605548152 cites W2118968205 @default.
• W2605548152 cites W2124871299 @default.
• W2605548152 cites W2130802972 @default.
• W2605548152 cites W2137500638 @default.
• W2605548152 cites W2137816710 @default.
• W2605548152 cites W2141507075 @default.
• W2605548152 cites W2147217940 @default.
• W2605548152 cites W2153983456 @default.
• W2605548152 cites W2154019529 @default.
• W2605548152 cites W2156174123 @default.
• W2605548152 cites W2168839470 @default.
• W2605548152 cites W2170965391 @default.
• W2605548152 cites W2171084650 @default.
• W2605548152 cites W2172340082 @default.
• W2605548152 cites W2203042236 @default.
• W2605548152 cites W2224705212 @default.
• W2605548152 cites W2264177798 @default.
• W2605548152 cites W2265921391 @default.
• W2605548152 cites W2307754412 @default.
• W2605548152 cites W2319356234 @default.
• W2605548152 cites W240618109 @default.
• W2605548152 cites W2409385548 @default.
• W2605548152 cites W2414624742 @default.
• W2605548152 cites W2465424457 @default.
• W2605548152 cites W2491263595 @default.
• W2605548152 cites W2503902293 @default.
• W2605548152 cites W257400002 @default.
• W2605548152 doi "https://doi.org/10.1074/jbc.m117.776211" @default.
• W2605548152 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5465483" @default.
• W2605548152 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28416612" @default.
• W2605548152 hasPublicationYear "2017" @default.
• W2605548152 type Work @default.
• W2605548152 sameAs 2605548152 @default.
• W2605548152 citedByCount "8" @default.
• W2605548152 countsByYear W26055481522017 @default.
• W2605548152 countsByYear W26055481522019 @default.
• W2605548152 countsByYear W26055481522020 @default.

• next