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- W260602016 abstract "PolyA/sup +/ mRNA was prepared from rabbit skeletal muscle. The mRNA was translated in vitro using a rabbit reticulocyte lysate system; the in vitro translation products were labelled using /sup 35/-methionine. The products were immunoprecipitated using a monoclonal antibody directed against phosphatase C-I. This enzyme preparation had previously been observed to have a molecular weight of 32 kDa. The immunoprecipitated products were examined by autoradiography after SDS-PAGE. The major immunoprecipitated polypeptides were of 38 kDa and 35 kDa. These findings indicate that the native size of phosphatase C-I is 38 kDa. These results are consistent with the finding in this and other laboratories that if the enzyme preparation is performed in the presence of protease inhibitors, a 38 kDa enzyme, rather than a 32 kDa enzyme is obtained." @default.
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- W260602016 date "1986-05-01" @default.
- W260602016 modified "2023-09-22" @default.
- W260602016 title "Native size of protein phosphatase C-I is 38 kDa as determined by immunoprecipitation of the in vitro translation products of total rabbit muscle mRNA" @default.
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