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• W2606025098 abstract "Abstract For the timely treatment of patients with infections in bloodstream and cerebrospinal fluid, a rapid antimicrobial susceptibility test (AST) is urgently needed. Here, we describe a direct and rapid antimicrobial susceptibility testing (dRAST) system, which can determine the antimicrobial susceptibility of bacteria from a positive blood culture bottle (PBCB) in six hours. The positive blood culture sample is directly mixed with agarose and inoculated into a micropatterned plastic microchip with lyophilized antibiotic agents. Using microscopic detection of bacterial colony formation in agarose, the total time to result from a PBCB for dRAST was only six hours for a wide range of bacterial concentrations in PBCBs. The results from the dRAST system were consistent with the results from a standard AST, broth microdilution test. In tests of clinical isolates (n = 206) composed of 16 Gram-negative species and seven Gram-positive species, the dRAST system was accurate compared to the standard broth microdilution test, with rates of 91.11% (2613/2868) categorical agreement, 6.69% (192/2868) minor error, 2.72% (50/1837) major error and 1.45% (13/896) very major error. Thus, the dRAST system can be used to rapidly identify appropriate antimicrobial agents for the treatment of blood stream infection (BSI) and antibiotic-resistant strain infections." @default.
• W2606025098 created "2017-04-28" @default.
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• W2606025098 date "2017-04-25" @default.
• W2606025098 modified "2023-10-17" @default.
• W2606025098 title "Direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis" @default.
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• W2606025098 doi "https://doi.org/10.1038/s41598-017-01278-2" @default.
• W2606025098 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5430693" @default.
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