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- W2606276177 abstract "Molecular dynamics of glutamate receptor, which is major neurotransmitter receptor at excitatory synapse located on neuron, is essential for synaptic plasticity in the complex neuronal networks. Here we studied molecular dynamics in an optical trap of AMPA-type glutamate receptor (AMPAR) labeled with quantum-dot (QD) on living neuronal cells with fluorescence imaging and fluorescence correlation spectroscopy (FCS). When a 1064-nm laser beam for optical trapping was focused on QD-AMPARs located on neuronal cells, the fluorescence intensity of QD-AMPARs gradually increased at the focal spot. Using single-particle tracking of QD-AMPARs on neurons, the average diffusion coefficient decreased in an optical trap. Moreover, the decay time obtained from FCS analysis increased with the laser power and the initial assembling state of AMPARs depended on culturing day, suggesting that the motion of QD-AMPAR was constrained in an optical trap." @default.
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- W2606276177 date "2017-04-18" @default.
- W2606276177 modified "2023-09-23" @default.
- W2606276177 title "Molecular dynamics in an optical trap of glutamate receptors labeled with quantum-dots on living neurons" @default.
- W2606276177 cites W1965374043 @default.
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- W2606276177 doi "https://doi.org/10.1117/12.2275026" @default.
- W2606276177 hasPublicationYear "2017" @default.
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