FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2606338945> ?p ?o ?g. }

• W2606338945 endingPage "407" @default.
• W2606338945 startingPage "394" @default.
• W2606338945 abstract "Summary Studies of protein N ‐glycosylation are important for answering fundamental questions on the diverse functions of glycoproteins in plant growth and development. Here we generated and characterised a comprehensive collection of Lotus japonicus LORE 1 insertion mutants, each lacking the activity of one of the 12 enzymes required for normal N ‐glycan maturation in the glycosylation machinery. The inactivation of the individual genes resulted in altered N ‐glycan patterns as documented using mass spectrometry and glycan‐recognising antibodies, indicating successful identification of null mutations in the target glyco‐genes. For example, both mass spectrometry and immunoblotting experiments suggest that proteins derived from the α1,3‐fucosyltransferase ( Lj3fuct ) mutant completely lacked α1,3‐core fucosylation. Mass spectrometry also suggested that the Lotus japonicus convicilin 2 was one of the main glycoproteins undergoing differential expression/ N ‐glycosylation in the mutants. Demonstrating the functional importance of glycosylation, reduced growth and seed production phenotypes were observed for the mutant plants lacking functional mannosidase I, N ‐acetylglucosaminyltransferase I, and α1,3‐fucosyltransferase, even though the relative protein composition and abundance appeared unaffected. The strength of our N ‐glycosylation mutant platform is the broad spectrum of resulting glycoprotein profiles and altered physiological phenotypes that can be produced from single, double, triple and quadruple mutants. This platform will serve as a valuable tool for elucidating the functional role of protein N ‐glycosylation in plants. Furthermore, this technology can be used to generate stable plant mutant lines for biopharmaceutical production of glycoproteins displaying relative homogeneous and mammalian‐like N ‐glycosylation features." @default.
• W2606338945 created "2017-04-28" @default.
• W2606338945 creator A5001462063 @default.
• W2606338945 creator A5008794391 @default.
• W2606338945 creator A5008993668 @default.
• W2606338945 creator A5023073170 @default.
• W2606338945 creator A5025069060 @default.
• W2606338945 creator A5037819491 @default.
• W2606338945 creator A5049027923 @default.
• W2606338945 creator A5060633945 @default.
• W2606338945 creator A5069523722 @default.
• W2606338945 creator A5078382029 @default.
• W2606338945 creator A5081164065 @default.
• W2606338945 creator A5085345265 @default.
• W2606338945 creator A5090521668 @default.
• W2606338945 date "2017-05-22" @default.
• W2606338945 modified "2023-10-14" @default.
• W2606338945 title "<i>N</i> -glycan maturation mutants in <i>Lotus japonicus</i> for basic and applied glycoprotein research" @default.
• W2606338945 cites W1246816926 @default.
• W2606338945 cites W1501951324 @default.
• W2606338945 cites W1678784904 @default.
• W2606338945 cites W1782270454 @default.
• W2606338945 cites W1951064865 @default.
• W2606338945 cites W1960053061 @default.
• W2606338945 cites W1965443132 @default.
• W2606338945 cites W1966887838 @default.
• W2606338945 cites W1972375269 @default.
• W2606338945 cites W1976484683 @default.
• W2606338945 cites W1977407568 @default.
• W2606338945 cites W1993838215 @default.
• W2606338945 cites W1998354192 @default.
• W2606338945 cites W2004125187 @default.
• W2606338945 cites W2006660640 @default.
• W2606338945 cites W2007069042 @default.
• W2606338945 cites W2016993376 @default.
• W2606338945 cites W2022922908 @default.
• W2606338945 cites W2026760618 @default.
• W2606338945 cites W2031624232 @default.
• W2606338945 cites W2033331583 @default.
• W2606338945 cites W2034833602 @default.
• W2606338945 cites W2034862072 @default.
• W2606338945 cites W2036493629 @default.
• W2606338945 cites W2037357013 @default.
• W2606338945 cites W2039409183 @default.
• W2606338945 cites W2041647620 @default.
• W2606338945 cites W2043863576 @default.
• W2606338945 cites W2046284765 @default.
• W2606338945 cites W2046902274 @default.
• W2606338945 cites W2072492117 @default.
• W2606338945 cites W2075816427 @default.
• W2606338945 cites W2078154253 @default.
• W2606338945 cites W2080567165 @default.
• W2606338945 cites W2081273693 @default.
• W2606338945 cites W2097975369 @default.
• W2606338945 cites W2098720815 @default.
• W2606338945 cites W2108874048 @default.
• W2606338945 cites W2109781401 @default.
• W2606338945 cites W2110031906 @default.
• W2606338945 cites W2112519609 @default.
• W2606338945 cites W2113835424 @default.
• W2606338945 cites W2114236610 @default.
• W2606338945 cites W2132545099 @default.
• W2606338945 cites W2135689611 @default.
• W2606338945 cites W2135931409 @default.
• W2606338945 cites W2136906707 @default.
• W2606338945 cites W2140615822 @default.
• W2606338945 cites W2143941673 @default.
• W2606338945 cites W2147215195 @default.
• W2606338945 cites W2152080491 @default.
• W2606338945 cites W2154763845 @default.
• W2606338945 cites W2157755881 @default.
• W2606338945 cites W2159235459 @default.
• W2606338945 cites W2164864818 @default.
• W2606338945 cites W2166363259 @default.
• W2606338945 cites W2190199302 @default.
• W2606338945 cites W2258049949 @default.
• W2606338945 cites W2342211743 @default.
• W2606338945 cites W2464790038 @default.
• W2606338945 cites W2465820953 @default.
• W2606338945 cites W66185224 @default.
• W2606338945 cites W993338644 @default.
• W2606338945 doi "https://doi.org/10.1111/tpj.13570" @default.
• W2606338945 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28407380" @default.
• W2606338945 hasPublicationYear "2017" @default.
• W2606338945 type Work @default.
• W2606338945 sameAs 2606338945 @default.
• W2606338945 citedByCount "23" @default.
• W2606338945 countsByYear W26063389452017 @default.
• W2606338945 countsByYear W26063389452018 @default.
• W2606338945 countsByYear W26063389452019 @default.
• W2606338945 countsByYear W26063389452020 @default.
• W2606338945 countsByYear W26063389452021 @default.
• W2606338945 countsByYear W26063389452022 @default.
• W2606338945 countsByYear W26063389452023 @default.
• W2606338945 crossrefType "journal-article" @default.
• W2606338945 hasAuthorship W2606338945A5001462063 @default.
• W2606338945 hasAuthorship W2606338945A5008794391 @default.
• W2606338945 hasAuthorship W2606338945A5008993668 @default.

• next