Matches in SemOpenAlex for { <https://semopenalex.org/work/W2607149820> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2607149820 endingPage "140" @default.
- W2607149820 startingPage "137" @default.
- W2607149820 abstract "We report the generation-characterization of a fetal liver (FL) B-cell progenitor (BCP)-derived human induced pluripotent stem cell (hiPSC) line CRISPR/Cas9-edited to carry/express a single copy of doxycycline-inducible Cas9 gene in the “safe locus” AAVS1 (iCas9-FL-BCP-hiPSC). Gene-edited iPSCs remained pluripotent after CRISPR/Cas9 genome-edition. Correct genomic integration of a unique copy of Cas9 was confirmed by PCR and Southern blot. Cas9 was robustly and specifically expressed on doxycycline exposure. T7-endonuclease assay demonstrated that iCas9 induces robust gene-edition when gRNAs against hematopoietic transcription factors were tested. This iCas9-FL-BCP-hiPSC will facilitate gene-editing approaches for studies on developmental biology, drug screening and disease modeling." @default.
- W2607149820 created "2017-04-28" @default.
- W2607149820 creator A5000226725 @default.
- W2607149820 creator A5003393614 @default.
- W2607149820 creator A5005617864 @default.
- W2607149820 creator A5025725620 @default.
- W2607149820 creator A5031510877 @default.
- W2607149820 creator A5051864161 @default.
- W2607149820 creator A5053728594 @default.
- W2607149820 creator A5056525976 @default.
- W2607149820 creator A5066955100 @default.
- W2607149820 creator A5079404620 @default.
- W2607149820 date "2017-05-01" @default.
- W2607149820 modified "2023-10-18" @default.
- W2607149820 title "Generation and characterization of a human iPSC cell line expressing inducible Cas9 in the “safe harbor” AAVS1 locus" @default.
- W2607149820 cites W1839614861 @default.
- W2607149820 cites W2003171404 @default.
- W2607149820 cites W2271701705 @default.
- W2607149820 cites W2521164667 @default.
- W2607149820 doi "https://doi.org/10.1016/j.scr.2017.04.011" @default.
- W2607149820 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5446316" @default.
- W2607149820 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28677529" @default.
- W2607149820 hasPublicationYear "2017" @default.
- W2607149820 type Work @default.
- W2607149820 sameAs 2607149820 @default.
- W2607149820 citedByCount "23" @default.
- W2607149820 countsByYear W26071498202018 @default.
- W2607149820 countsByYear W26071498202019 @default.
- W2607149820 countsByYear W26071498202020 @default.
- W2607149820 countsByYear W26071498202021 @default.
- W2607149820 countsByYear W26071498202022 @default.
- W2607149820 countsByYear W26071498202023 @default.
- W2607149820 crossrefType "journal-article" @default.
- W2607149820 hasAuthorship W2607149820A5000226725 @default.
- W2607149820 hasAuthorship W2607149820A5003393614 @default.
- W2607149820 hasAuthorship W2607149820A5005617864 @default.
- W2607149820 hasAuthorship W2607149820A5025725620 @default.
- W2607149820 hasAuthorship W2607149820A5031510877 @default.
- W2607149820 hasAuthorship W2607149820A5051864161 @default.
- W2607149820 hasAuthorship W2607149820A5053728594 @default.
- W2607149820 hasAuthorship W2607149820A5056525976 @default.
- W2607149820 hasAuthorship W2607149820A5066955100 @default.
- W2607149820 hasAuthorship W2607149820A5079404620 @default.
- W2607149820 hasBestOaLocation W26071498201 @default.
- W2607149820 hasConcept C104317684 @default.
- W2607149820 hasConcept C107459253 @default.
- W2607149820 hasConcept C132455925 @default.
- W2607149820 hasConcept C144501496 @default.
- W2607149820 hasConcept C145103041 @default.
- W2607149820 hasConcept C153911025 @default.
- W2607149820 hasConcept C31909778 @default.
- W2607149820 hasConcept C54355233 @default.
- W2607149820 hasConcept C70721500 @default.
- W2607149820 hasConcept C84597430 @default.
- W2607149820 hasConcept C86803240 @default.
- W2607149820 hasConcept C95444343 @default.
- W2607149820 hasConcept C98108389 @default.
- W2607149820 hasConceptScore W2607149820C104317684 @default.
- W2607149820 hasConceptScore W2607149820C107459253 @default.
- W2607149820 hasConceptScore W2607149820C132455925 @default.
- W2607149820 hasConceptScore W2607149820C144501496 @default.
- W2607149820 hasConceptScore W2607149820C145103041 @default.
- W2607149820 hasConceptScore W2607149820C153911025 @default.
- W2607149820 hasConceptScore W2607149820C31909778 @default.
- W2607149820 hasConceptScore W2607149820C54355233 @default.
- W2607149820 hasConceptScore W2607149820C70721500 @default.
- W2607149820 hasConceptScore W2607149820C84597430 @default.
- W2607149820 hasConceptScore W2607149820C86803240 @default.
- W2607149820 hasConceptScore W2607149820C95444343 @default.
- W2607149820 hasConceptScore W2607149820C98108389 @default.
- W2607149820 hasLocation W26071498201 @default.
- W2607149820 hasLocation W26071498202 @default.
- W2607149820 hasLocation W26071498203 @default.
- W2607149820 hasLocation W26071498204 @default.
- W2607149820 hasLocation W26071498205 @default.
- W2607149820 hasLocation W26071498206 @default.
- W2607149820 hasOpenAccess W2607149820 @default.
- W2607149820 hasPrimaryLocation W26071498201 @default.
- W2607149820 hasRelatedWork W2134268326 @default.
- W2607149820 hasRelatedWork W2177685571 @default.
- W2607149820 hasRelatedWork W2547051899 @default.
- W2607149820 hasRelatedWork W2603161682 @default.
- W2607149820 hasRelatedWork W2667943050 @default.
- W2607149820 hasRelatedWork W2807464733 @default.
- W2607149820 hasRelatedWork W2903635918 @default.
- W2607149820 hasRelatedWork W2960163646 @default.
- W2607149820 hasRelatedWork W3120857162 @default.
- W2607149820 hasRelatedWork W3199043689 @default.
- W2607149820 hasVolume "21" @default.
- W2607149820 isParatext "false" @default.
- W2607149820 isRetracted "false" @default.
- W2607149820 magId "2607149820" @default.
- W2607149820 workType "article" @default.