Matches in SemOpenAlex for { <https://semopenalex.org/work/W2607156656> ?p ?o ?g. }
- W2607156656 endingPage "3258" @default.
- W2607156656 startingPage "3247" @default.
- W2607156656 abstract "7-Ethyl-10-hydroxycamptothecin (SN38), as a highly active topoisomerase I inhibitor, is 200-2000-fold more cytotoxic than irinotecan (CPT-11) commercially available as Camptosar®. However, poor solubility and low stability extensively restricted its clinical utility. In this report, dual SN38 phospholipid conjugate (Di-SN38-PC) prodrug based liposomes were developed in order to compact these drawbacks. Di-SN38-PC prodrug was first synthesized by inhomogeneous conjugation of two SN38-20-O-succinic acid molecules with L-α-glycerophosphorylcholine (GPC). The assembly of the prodrug was carried out without any excipient by using thin film method. Dynamic light scattering (DLS), transmission electron microscope (TEM) and cryogenic transmission electron microscopy (cyro-TEM) characterization indicated that Di-SN38-PC can form spherical liposomes with narrow particle size (<200nm) and negatively charged surface (-21.6±3.5mV). The loading efficiency of SN38 is 65.2 wt.% after a simple calculation. In vitro release test was further performed in detail. The results demonstrated that Di-SN38-PC liposomes were stable in neutral environment but degraded in a weakly acidic condition thereby released parent drug SN38 effectively. Cellular uptake studies reflected that the liposomes could be internalized into cells more significantly than SN38. In vitro antitumor activities were finally evaluated by MTT assay, colony formation assay, flow cytometry, RT-PCR analysis and Western Blot. The results showed that Di-SN38-PC liposomes had a comparable cytotoxicity with SN38 against MCF-7 and HBL-100, and a selective promotion of apoptosis of tumor cells. Furthermore, a pharmacokinetics test showed that Di-SN38-PC liposomes had a longer circulating time in blood compared with the parent drug. All the results indicate that Di-SN38-PC liposomes are an effective delivery system of SN38." @default.
- W2607156656 created "2017-04-28" @default.
- W2607156656 creator A5002145855 @default.
- W2607156656 creator A5006407420 @default.
- W2607156656 creator A5014936514 @default.
- W2607156656 creator A5025897218 @default.
- W2607156656 creator A5029608114 @default.
- W2607156656 creator A5047932271 @default.
- W2607156656 creator A5068337005 @default.
- W2607156656 creator A5087884387 @default.
- W2607156656 date "2017-06-01" @default.
- W2607156656 modified "2023-09-25" @default.
- W2607156656 title "Dual 7-ethyl-10-hydroxycamptothecin conjugated phospholipid prodrug assembled liposomes with in vitro anticancer effects" @default.
- W2607156656 cites W1795178315 @default.
- W2607156656 cites W1842519401 @default.
- W2607156656 cites W1883091920 @default.
- W2607156656 cites W1894737231 @default.
- W2607156656 cites W1965278354 @default.
- W2607156656 cites W1967110146 @default.
- W2607156656 cites W1970017618 @default.
- W2607156656 cites W1980419364 @default.
- W2607156656 cites W1980521536 @default.
- W2607156656 cites W1981141192 @default.
- W2607156656 cites W1991029054 @default.
- W2607156656 cites W1994399615 @default.
- W2607156656 cites W1995742898 @default.
- W2607156656 cites W2002134219 @default.
- W2607156656 cites W2004313264 @default.
- W2607156656 cites W2008160002 @default.
- W2607156656 cites W2010113256 @default.
- W2607156656 cites W2010781083 @default.
- W2607156656 cites W2024382973 @default.
- W2607156656 cites W2030184227 @default.
- W2607156656 cites W2034915619 @default.
- W2607156656 cites W2036337806 @default.
- W2607156656 cites W2038451015 @default.
- W2607156656 cites W2038720439 @default.
- W2607156656 cites W2042018154 @default.
- W2607156656 cites W2053622985 @default.
- W2607156656 cites W2064788032 @default.
- W2607156656 cites W2065946609 @default.
- W2607156656 cites W2075062190 @default.
- W2607156656 cites W2092652665 @default.
- W2607156656 cites W2100917151 @default.
- W2607156656 cites W2117655985 @default.
- W2607156656 cites W2142564546 @default.
- W2607156656 cites W2143463421 @default.
- W2607156656 cites W2159583680 @default.
- W2607156656 cites W2160624529 @default.
- W2607156656 cites W2224759842 @default.
- W2607156656 cites W2274657786 @default.
- W2607156656 cites W2278428896 @default.
- W2607156656 cites W2300671107 @default.
- W2607156656 cites W2336792134 @default.
- W2607156656 cites W4233567369 @default.
- W2607156656 cites W61782866 @default.
- W2607156656 doi "https://doi.org/10.1016/j.bmc.2017.04.025" @default.
- W2607156656 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28465086" @default.
- W2607156656 hasPublicationYear "2017" @default.
- W2607156656 type Work @default.
- W2607156656 sameAs 2607156656 @default.
- W2607156656 citedByCount "29" @default.
- W2607156656 countsByYear W26071566562017 @default.
- W2607156656 countsByYear W26071566562018 @default.
- W2607156656 countsByYear W26071566562019 @default.
- W2607156656 countsByYear W26071566562020 @default.
- W2607156656 countsByYear W26071566562021 @default.
- W2607156656 countsByYear W26071566562022 @default.
- W2607156656 countsByYear W26071566562023 @default.
- W2607156656 crossrefType "journal-article" @default.
- W2607156656 hasAuthorship W2607156656A5002145855 @default.
- W2607156656 hasAuthorship W2607156656A5006407420 @default.
- W2607156656 hasAuthorship W2607156656A5014936514 @default.
- W2607156656 hasAuthorship W2607156656A5025897218 @default.
- W2607156656 hasAuthorship W2607156656A5029608114 @default.
- W2607156656 hasAuthorship W2607156656A5047932271 @default.
- W2607156656 hasAuthorship W2607156656A5068337005 @default.
- W2607156656 hasAuthorship W2607156656A5087884387 @default.
- W2607156656 hasConcept C108215921 @default.
- W2607156656 hasConcept C109316439 @default.
- W2607156656 hasConcept C12554922 @default.
- W2607156656 hasConcept C134306372 @default.
- W2607156656 hasConcept C14631669 @default.
- W2607156656 hasConcept C155672457 @default.
- W2607156656 hasConcept C171250308 @default.
- W2607156656 hasConcept C185154212 @default.
- W2607156656 hasConcept C185592680 @default.
- W2607156656 hasConcept C192562407 @default.
- W2607156656 hasConcept C197336794 @default.
- W2607156656 hasConcept C202751555 @default.
- W2607156656 hasConcept C2778918659 @default.
- W2607156656 hasConcept C2779682216 @default.
- W2607156656 hasConcept C2780783641 @default.
- W2607156656 hasConcept C33923547 @default.
- W2607156656 hasConcept C41625074 @default.
- W2607156656 hasConcept C55493867 @default.
- W2607156656 hasConcept C86803240 @default.
- W2607156656 hasConceptScore W2607156656C108215921 @default.