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- W2607447732 abstract "Preparations that contain well-spread metaphase chromosomes are critical for plant cytogenetic analyses including chromosome counts, banding procedures, in situ hybridization, karyotyping and construction of ideograms. Chromosome spreading is difficult for plants with large and numerous chromosomes. We report here a technique for obtaining cytoplasm-free, well-spread metaphases from two Amaryllidaceae species: Sprekelia formosissima (2n = 120) and Hymenocallis howardii (2n = 96). The technique has three main steps: 1) pretreatment to cause chromosome condensation, 2) dripping onto tilted slides coated with a thin layer of pure acetic acid and 3) application of steam and acetic acid to produce cytoplasmic hydrolysis, which spreads the chromosomes." @default.
- W2607447732 created "2017-04-28" @default.
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- W2607447732 date "2017-03-01" @default.
- W2607447732 modified "2023-09-27" @default.
- W2607447732 title "An improved technique for obtaining well-spread metaphases from plants with numerous large chromosomes" @default.
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- W2607447732 doi "https://doi.org/10.1080/10520295.2017.1288927" @default.
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