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• W2607937729 abstract "Recent sequencing revolution driven by high-throughput technologies has led to rapid accumulation of 16S rRNA sequences for microbial communities. Clustering short sequences into operational taxonomic units (OTUs) is an initial crucial process in analyzing metagenomic data. Although many heuristic methods have been proposed for OTU inferences with low computational complexity, they just select one sequence as the seed for each cluster and the results are sensitive to the selected sequences that represent the clusters. To address this issue, we present a de Bruijn graph-based heuristic clustering method (DBH) for clustering massive 16S rRNA sequences into OTUs by introducing a novel seed selection strategy and greedy clustering approach. Compared with existing widely used methods on several simulated and real-life metagenomic datasets, the results show that DBH has higher clustering performance and low memory usage, facilitating the overestimation of OTUs number. DBH is more effective to handle large-scale metagenomic datasets. The DBH software can be freely downloaded from https://github.com/nwpu134/DBH.git for academic users." @default.
• W2607937729 created "2017-05-05" @default.
• W2607937729 creator A5061712236 @default.
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• W2607937729 date "2017-07-01" @default.
• W2607937729 modified "2023-10-15" @default.
• W2607937729 title "DBH: A de Bruijn graph-based heuristic method for clustering large-scale 16S rRNA sequences into OTUs" @default.
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• W2607937729 doi "https://doi.org/10.1016/j.jtbi.2017.04.019" @default.
• W2607937729 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28454900" @default.
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