FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2608328137> ?p ?o ?g. }

• W2608328137 abstract "Toxoplasma gondii is known to cause congenital infection in humans and animals and severe disease in immunocompromised individuals; consequently development of vaccines against the parasite is highly necessary. Under stress conditions, T. gondii expresses the highly immunogenic heat shock protein 70 (TgHSP70). Here, we assessed the protective efficacy of rTgHSP70 immunization combined with Alum in oral ME-49 T. gondii infection and the mechanisms involved on it. It was observed that immunized mice with rTgHSP70 or rTgHSP70 adsorbed in Alum presented a significantly reduced number of cysts in the brain that was associated with increased iNOS+ cell numbers in the organ, irrespective the use of the adjuvant. Indeed, ex vivo experiments showed that peritoneal macrophages pre-stimulated with rTgHSP70 presented increased NO production and enhanced parasite killing, and the protein was able to directly stimulate B cells toward antibody producing profile. In addition, rTgHSP70 immunization leads to high specific antibody titters systemically and a mixed IgG1/IgG2a response, with predominance of IgG1 production. Nonetheless, it was observed that the pretreatment of the parasite with rTgHSP70 immune sera was not able to control T. gondii internalization and replication by NIH fibroblast neither peritoneal murine macrophages, nor anti-rTgHSP70 antibodies were able to kill T. gondii by complement-mediated lysis, suggesting that these mechanisms are not crucial to resistance. Interestingly, when in combination with Alum, rTgHSP70 immunization was able to reduce inflammation in the brain of infected mice and in parallel anti-rTgHSP70 immune complexes in the serum. In conclusion, immunization with rTgHSP70 induces massive amounts of iNOS expression and reduced brain parasitism, suggesting that iNOS expression and consequently NO production in the brain is a protective mechanism induced by TgHSP70 immunization, therefore rTgHSP70 can be a good candidate for vaccine development against toxoplasmosis." @default.
• W2608328137 created "2017-05-05" @default.
• W2608328137 creator A5007040642 @default.
• W2608328137 creator A5036586362 @default.
• W2608328137 creator A5050690651 @default.
• W2608328137 creator A5056284263 @default.
• W2608328137 creator A5087779686 @default.
• W2608328137 date "2017-04-25" @default.
• W2608328137 modified "2023-10-14" @default.
• W2608328137 title "Recombinant TgHSP70 Immunization Protects against Toxoplasma gondii Brain Cyst Formation by Enhancing Inducible Nitric Oxide Expression" @default.
• W2608328137 cites W102997368 @default.
• W2608328137 cites W1510294526 @default.
• W2608328137 cites W1521556649 @default.
• W2608328137 cites W1528893442 @default.
• W2608328137 cites W1545910465 @default.
• W2608328137 cites W1569584355 @default.
• W2608328137 cites W1592104297 @default.
• W2608328137 cites W1665796496 @default.
• W2608328137 cites W1866964444 @default.
• W2608328137 cites W1888830013 @default.
• W2608328137 cites W1895952713 @default.
• W2608328137 cites W1898034247 @default.
• W2608328137 cites W1905006012 @default.
• W2608328137 cites W1928749038 @default.
• W2608328137 cites W1958163415 @default.
• W2608328137 cites W1969600123 @default.
• W2608328137 cites W1970279912 @default.
• W2608328137 cites W1975123569 @default.
• W2608328137 cites W1975938678 @default.
• W2608328137 cites W1978448373 @default.
• W2608328137 cites W1983430686 @default.
• W2608328137 cites W1992653134 @default.
• W2608328137 cites W1994590893 @default.
• W2608328137 cites W1996485781 @default.
• W2608328137 cites W1998047654 @default.
• W2608328137 cites W2003158331 @default.
• W2608328137 cites W2004955321 @default.
• W2608328137 cites W2009521410 @default.
• W2608328137 cites W2027447559 @default.
• W2608328137 cites W2028500185 @default.
• W2608328137 cites W2029564173 @default.
• W2608328137 cites W2030719695 @default.
• W2608328137 cites W2034320715 @default.
• W2608328137 cites W2034357061 @default.
• W2608328137 cites W2042990958 @default.
• W2608328137 cites W2054519950 @default.
• W2608328137 cites W2061164005 @default.
• W2608328137 cites W2062391630 @default.
• W2608328137 cites W2066996032 @default.
• W2608328137 cites W2072676033 @default.
• W2608328137 cites W2078909808 @default.
• W2608328137 cites W2079632476 @default.
• W2608328137 cites W2080582389 @default.
• W2608328137 cites W2083648297 @default.
• W2608328137 cites W2084165419 @default.
• W2608328137 cites W2087247963 @default.
• W2608328137 cites W2090883923 @default.
• W2608328137 cites W2094758239 @default.
• W2608328137 cites W2098679813 @default.
• W2608328137 cites W2099410504 @default.
• W2608328137 cites W2105347339 @default.
• W2608328137 cites W2106093190 @default.
• W2608328137 cites W2108323291 @default.
• W2608328137 cites W2109480257 @default.
• W2608328137 cites W2110145073 @default.
• W2608328137 cites W2111253543 @default.
• W2608328137 cites W2112916588 @default.
• W2608328137 cites W2117079340 @default.
• W2608328137 cites W2120253175 @default.
• W2608328137 cites W2120482236 @default.
• W2608328137 cites W2131861425 @default.
• W2608328137 cites W2132304929 @default.
• W2608328137 cites W2135908101 @default.
• W2608328137 cites W2144244283 @default.
• W2608328137 cites W2145842207 @default.
• W2608328137 cites W2146771667 @default.
• W2608328137 cites W2163443542 @default.
• W2608328137 cites W2168387989 @default.
• W2608328137 cites W2179377276 @default.
• W2608328137 cites W2209894880 @default.
• W2608328137 cites W2300502443 @default.
• W2608328137 cites W2342289251 @default.
• W2608328137 cites W2342989057 @default.
• W2608328137 cites W2400666768 @default.
• W2608328137 cites W2550086924 @default.
• W2608328137 cites W2570988623 @default.
• W2608328137 cites W4210504714 @default.
• W2608328137 doi "https://doi.org/10.3389/fcimb.2017.00142" @default.
• W2608328137 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5403831" @default.
• W2608328137 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28487847" @default.
• W2608328137 hasPublicationYear "2017" @default.
• W2608328137 type Work @default.
• W2608328137 sameAs 2608328137 @default.
• W2608328137 citedByCount "27" @default.
• W2608328137 countsByYear W26083281372017 @default.
• W2608328137 countsByYear W26083281372018 @default.
• W2608328137 countsByYear W26083281372019 @default.
• W2608328137 countsByYear W26083281372021 @default.
• W2608328137 countsByYear W26083281372022 @default.
• W2608328137 countsByYear W26083281372023 @default.

• next