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- W2608640147 endingPage "e0176333" @default.
- W2608640147 startingPage "e0176333" @default.
- W2608640147 abstract "microRNAs (miRNAs) are derived from self-complementary hairpin structures, while small-interfering RNAs (siRNAs) are derived from double-stranded RNA (dsRNA) or hairpin precursors. The core mechanism of sRNA production involves DICER-like (DCL) in processing the smallRNAs (sRNAs) and ARGONAUTE (AGO) as effectors of silencing, and siRNA biogenesis also involves action of RNA-Dependent RNA Polymerase (RDR), Pol IV and Pol V in biogenesis. Several other proteins interact with the core proteins to guide sRNA biogenesis, action, and turnover. We aimed to unravel the components and functions of the RNA-guided silencing pathway in a non-model plant species of worldwide economic relevance. The sRNA-guided silencing complex members have been identified in the Coffea canephora genome, and they have been characterized at the structural, functional, and evolutionary levels by computational analyses. Eleven AGO proteins, nine DCL proteins (which include a DCL1-like protein that was not previously annotated), and eight RDR proteins were identified. Another 48 proteins implicated in smallRNA (sRNA) pathways were also identified. Furthermore, we identified 235 miRNA precursors and 317 mature miRNAs from 113 MIR families, and we characterized ccp-MIR156, ccp-MIR172, and ccp-MIR390. Target prediction and gene ontology analyses of 2239 putative targets showed that significant pathways in coffee are targeted by miRNAs. We provide evidence of the expansion of the loci related to sRNA pathways, insights into the activities of these proteins by domain and catalytic site analyses, and gene expression analysis. The number of MIR loci and their targeted pathways highlight the importance of miRNAs in coffee. We identified several roles of sRNAs in C. canephora, which offers substantial insight into better understanding the transcriptional and post-transcriptional regulation of this major crop." @default.
- W2608640147 created "2017-05-05" @default.
- W2608640147 creator A5008274219 @default.
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- W2608640147 creator A5062044626 @default.
- W2608640147 creator A5076577040 @default.
- W2608640147 creator A5087621082 @default.
- W2608640147 date "2017-04-27" @default.
- W2608640147 modified "2023-09-27" @default.
- W2608640147 title "A genome-wide analysis of the RNA-guided silencing pathway in coffee reveals insights into its regulatory mechanisms" @default.
- W2608640147 cites W1191273493 @default.
- W2608640147 cites W1567381189 @default.
- W2608640147 cites W1568987713 @default.
- W2608640147 cites W1590790765 @default.
- W2608640147 cites W1592870802 @default.
- W2608640147 cites W1643740851 @default.
- W2608640147 cites W1824345792 @default.
- W2608640147 cites W1967761184 @default.
- W2608640147 cites W1972999456 @default.
- W2608640147 cites W1976303288 @default.
- W2608640147 cites W1976909997 @default.
- W2608640147 cites W1977851636 @default.
- W2608640147 cites W1984251741 @default.
- W2608640147 cites W1984891757 @default.
- W2608640147 cites W1987603586 @default.
- W2608640147 cites W1991638844 @default.
- W2608640147 cites W1992512552 @default.
- W2608640147 cites W1993180746 @default.
- W2608640147 cites W1997902480 @default.
- W2608640147 cites W1998138469 @default.
- W2608640147 cites W2007959167 @default.
- W2608640147 cites W2008057145 @default.
- W2608640147 cites W2008391336 @default.
- W2608640147 cites W2009206848 @default.
- W2608640147 cites W2009540277 @default.
- W2608640147 cites W2010541841 @default.
- W2608640147 cites W2011419478 @default.
- W2608640147 cites W2012374969 @default.
- W2608640147 cites W2018119263 @default.
- W2608640147 cites W2020458511 @default.
- W2608640147 cites W2026343134 @default.
- W2608640147 cites W2030989926 @default.
- W2608640147 cites W2038120864 @default.
- W2608640147 cites W2038585600 @default.
- W2608640147 cites W2041985441 @default.
- W2608640147 cites W2042325491 @default.
- W2608640147 cites W2051728363 @default.
- W2608640147 cites W2056196527 @default.
- W2608640147 cites W2056546931 @default.
- W2608640147 cites W2057162139 @default.
- W2608640147 cites W2059291537 @default.
- W2608640147 cites W2063309481 @default.
- W2608640147 cites W2065461553 @default.
- W2608640147 cites W2066464349 @default.
- W2608640147 cites W2071541955 @default.
- W2608640147 cites W2071929044 @default.
- W2608640147 cites W2072017031 @default.
- W2608640147 cites W2073017209 @default.
- W2608640147 cites W2078680011 @default.
- W2608640147 cites W2079320075 @default.
- W2608640147 cites W2092940479 @default.
- W2608640147 cites W2095750709 @default.
- W2608640147 cites W2096228494 @default.
- W2608640147 cites W2097595476 @default.
- W2608640147 cites W2097706568 @default.
- W2608640147 cites W2098407761 @default.
- W2608640147 cites W2111092051 @default.
- W2608640147 cites W2122135986 @default.
- W2608640147 cites W2122889796 @default.
- W2608640147 cites W2123687559 @default.
- W2608640147 cites W2126419817 @default.
- W2608640147 cites W2127223135 @default.
- W2608640147 cites W2130575465 @default.
- W2608640147 cites W2131326486 @default.
- W2608640147 cites W2132632499 @default.
- W2608640147 cites W2137015675 @default.
- W2608640147 cites W2137915351 @default.
- W2608640147 cites W2140415861 @default.
- W2608640147 cites W2142234360 @default.
- W2608640147 cites W2143593801 @default.
- W2608640147 cites W2144177541 @default.
- W2608640147 cites W2151476452 @default.
- W2608640147 cites W2151953896 @default.
- W2608640147 cites W2152490984 @default.
- W2608640147 cites W2154257567 @default.
- W2608640147 cites W2158079305 @default.
- W2608640147 cites W2160742085 @default.
- W2608640147 cites W2166265186 @default.
- W2608640147 cites W2166795672 @default.
- W2608640147 cites W2167681032 @default.
- W2608640147 cites W2193801262 @default.
- W2608640147 cites W2195136000 @default.
- W2608640147 cites W2204139220 @default.
- W2608640147 cites W2257765417 @default.
- W2608640147 cites W2299628074 @default.