Matches in SemOpenAlex for { <https://semopenalex.org/work/W2608759419> ?p ?o ?g. }
- W2608759419 abstract "Clustering of the T-cell receptor (TCR) is thought to initiate downstream signalling. However, the detection of protein clustering with high spatial and temporal resolution remains challenging. Here we establish a Förster resonance energy transfer (FRET) sensor, named CliF, which reports intermolecular associations of neighbouring proteins in live cells. A key advantage of the single-chain FRET sensor is that it can be combined with image correlation spectroscopy (ICS), single-particle tracking (SPT) and fluorescence lifetime imaging microscopy (FLIM). We test the sensor with a light-sensitive actuator that induces protein aggregation upon radiation with blue light. When applied to T cells, the sensor reveals that TCR triggering increases the number of dense TCR-CD3 clusters. Further, we find a correlation between cluster movement within the immunological synapse and cluster density. In conclusion, we develop a sensor that allows us to map the dynamics of protein clustering in live T cells." @default.
- W2608759419 created "2017-05-05" @default.
- W2608759419 creator A5007943885 @default.
- W2608759419 creator A5013388371 @default.
- W2608759419 creator A5024443532 @default.
- W2608759419 creator A5024491415 @default.
- W2608759419 creator A5026262726 @default.
- W2608759419 creator A5043225523 @default.
- W2608759419 creator A5068674136 @default.
- W2608759419 creator A5077101610 @default.
- W2608759419 creator A5082023190 @default.
- W2608759419 date "2017-04-01" @default.
- W2608759419 modified "2023-10-15" @default.
- W2608759419 title "An intermolecular FRET sensor detects the dynamics of T cell receptor clustering" @default.
- W2608759419 cites W1215621103 @default.
- W2608759419 cites W1601195737 @default.
- W2608759419 cites W1970635900 @default.
- W2608759419 cites W1973709420 @default.
- W2608759419 cites W1974374103 @default.
- W2608759419 cites W1979984478 @default.
- W2608759419 cites W1980299712 @default.
- W2608759419 cites W1988783201 @default.
- W2608759419 cites W1991409871 @default.
- W2608759419 cites W1993687714 @default.
- W2608759419 cites W1997613020 @default.
- W2608759419 cites W2001395998 @default.
- W2608759419 cites W2003871339 @default.
- W2608759419 cites W2005195616 @default.
- W2608759419 cites W2009656293 @default.
- W2608759419 cites W2015832765 @default.
- W2608759419 cites W2023930071 @default.
- W2608759419 cites W2024681724 @default.
- W2608759419 cites W2031264620 @default.
- W2608759419 cites W2043848880 @default.
- W2608759419 cites W2043891370 @default.
- W2608759419 cites W2049345986 @default.
- W2608759419 cites W2050226576 @default.
- W2608759419 cites W2055698508 @default.
- W2608759419 cites W2064050383 @default.
- W2608759419 cites W2070495940 @default.
- W2608759419 cites W2079594003 @default.
- W2608759419 cites W2083483039 @default.
- W2608759419 cites W2084590264 @default.
- W2608759419 cites W2088072996 @default.
- W2608759419 cites W2089805318 @default.
- W2608759419 cites W2090687717 @default.
- W2608759419 cites W2096621567 @default.
- W2608759419 cites W2100570806 @default.
- W2608759419 cites W2123186661 @default.
- W2608759419 cites W2126873850 @default.
- W2608759419 cites W2135630804 @default.
- W2608759419 cites W2135897043 @default.
- W2608759419 cites W2138128599 @default.
- W2608759419 cites W2139970775 @default.
- W2608759419 cites W2151497263 @default.
- W2608759419 cites W2156393136 @default.
- W2608759419 cites W2160420489 @default.
- W2608759419 cites W2167064790 @default.
- W2608759419 cites W2193239553 @default.
- W2608759419 cites W2295382293 @default.
- W2608759419 cites W2399861991 @default.
- W2608759419 doi "https://doi.org/10.1038/ncomms15100" @default.
- W2608759419 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5414349" @default.
- W2608759419 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28452360" @default.
- W2608759419 hasPublicationYear "2017" @default.
- W2608759419 type Work @default.
- W2608759419 sameAs 2608759419 @default.
- W2608759419 citedByCount "49" @default.
- W2608759419 countsByYear W26087594192017 @default.
- W2608759419 countsByYear W26087594192018 @default.
- W2608759419 countsByYear W26087594192019 @default.
- W2608759419 countsByYear W26087594192020 @default.
- W2608759419 countsByYear W26087594192021 @default.
- W2608759419 countsByYear W26087594192022 @default.
- W2608759419 countsByYear W26087594192023 @default.
- W2608759419 crossrefType "journal-article" @default.
- W2608759419 hasAuthorship W2608759419A5007943885 @default.
- W2608759419 hasAuthorship W2608759419A5013388371 @default.
- W2608759419 hasAuthorship W2608759419A5024443532 @default.
- W2608759419 hasAuthorship W2608759419A5024491415 @default.
- W2608759419 hasAuthorship W2608759419A5026262726 @default.
- W2608759419 hasAuthorship W2608759419A5043225523 @default.
- W2608759419 hasAuthorship W2608759419A5068674136 @default.
- W2608759419 hasAuthorship W2608759419A5077101610 @default.
- W2608759419 hasAuthorship W2608759419A5082023190 @default.
- W2608759419 hasBestOaLocation W26087594191 @default.
- W2608759419 hasConcept C121332964 @default.
- W2608759419 hasConcept C12554922 @default.
- W2608759419 hasConcept C144618238 @default.
- W2608759419 hasConcept C154945302 @default.
- W2608759419 hasConcept C164866538 @default.
- W2608759419 hasConcept C185592680 @default.
- W2608759419 hasConcept C186060115 @default.
- W2608759419 hasConcept C19317047 @default.
- W2608759419 hasConcept C199360897 @default.
- W2608759419 hasConcept C2776090121 @default.
- W2608759419 hasConcept C41008148 @default.
- W2608759419 hasConcept C54355233 @default.
- W2608759419 hasConcept C62520636 @default.
- W2608759419 hasConcept C73555534 @default.