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- W2608793959 abstract "Vibrio parahaemolyticus is an inhabitant of marine and estuarine environments and causes seafood-borne gastroenteritis in humans. In this study, an UltraFast LabChip Real-Time PCR assay was evaluated for rapid detection and quantification of pathogenic V. parahaemolyticus isolates. Escherichia coli and Vibrio harveyi were used as negative controls. Twenty-six tdh-positive, biofilm-producing V. parahaemolyticus isolates were analyzed by repetitive extragenic palindromic-polymerase chain reaction (REP-PCR). REP-PCR analysis showed that the majority of the V. parahaemolyticus isolates originated from seafood and that clinical specimens formed two major clusters at 92.8% and 32% similarity levels. The presence and quantification of Autoinducer-2 was carried out using high-performance liquid chromatography with fluorescence detection (HPLC-FLD) after derivatization of Autoinducer-2 with 2, 3-diaminonaphthalene. The presence of tdh-positive V. parahaemolyticus in marine samples highlights the need for constant environmental monitoring to protect public health." @default.
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- W2608793959 date "2017-04-28" @default.
- W2608793959 modified "2023-10-11" @default.
- W2608793959 title "Molecular characteristics, biofilm-forming abilities, and quorum sensing molecules in <i>Vibrio parahaemolyticus</i> strains isolated from marine and clinical environments in Korea" @default.
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- W2608793959 doi "https://doi.org/10.1080/08927014.2017.1316840" @default.
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