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- W2608820007 abstract "The aim of present in vivo study was to investigate the antioxidant, antiproliferative and induction of apoptosis by Phlomis Samia Methanolic Etract (PHSME) which traditional herbal medicine from Algeria. Quantitative determination of total phenolics and total flavonoids was carried out using colorimetric methods. The total phenolic content was found to be 73,14 mg of gallic acid equivalent per gram of extract, while the content of flavonoid show a value of 21,61; 46,97 mg of quercetin, ritin equivalent per gram of extract. The antioxidant activity was evaluated in vitro with the use of free radical scavenging activity method by DPPH assay, Linoleic acid peroxidation inhibition, Estimation of total reducing power, Estimation of _OH. scavenging, Lipid peroxidation inhibition assay, results indicated that dose depend% reduction againts the previous experiments mentioned. The anticancer activity was evaluated by anti-proliferation activity with MTT assay on tow kind of cells line HepG2 and MDA. The proliferation of HepG2, MDA was significantly decreased after 48 h, 72 h incubation with PHSME at 450 ug/ml. also apoptosis and celle cycle activity were evaluated by flow cytometry methods. Apoptosis observed in Annexin V-FTTCand propidium iodide (PI) staining of cells treated with PHSME for 48 h in HepG2 MDA cells. Morover PHSME arrest cell cycle progression in G1 phase, the anticancer activity of PHSME has very potent anticancer activities, which might be useful in liver and breast cancer." @default.
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- W2608820007 date "2017-01-01" @default.
- W2608820007 modified "2023-09-23" @default.
- W2608820007 title "Antioxidant, Anti-Proliferative, and Induction of Apoptosis by Phlomis samia Methanolic Extract from Algeria" @default.
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