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- W2609502575 abstract "Palabras clave: BChE, colinesterasa, plasma, perro, analisis Abstract: Comparison of three methods for the determination of plasma cholinesterase activity in dog. The determination of cholinesterase activity is important in the diagnosis and monitoring of poisoning by anticholinesterase pesticides (organophosphates and carbamates). The aim of this work was to study the influence of different methodologies in the determination of normal activity values of this enzyme in canine plasma. Samples from 141 dogs were analyzed for butyrylcholinesterase (BChE) activity to test the influence of the chromophore in the reaction and the differences in the results by using commercial kits or reagents prepared in the laboratory. The results were similar for the different methods. With DTNB as chromophore mean values were 3276 ± 845 UI/L using reagents prepared in the lab and 3013 ± 1081 UI/L using a commercial kit. With hexacyanoferrate III as chromophore mean value was 3330 ± 1184 UI/L. The buffer concentration, the pH of the medium and the chromophore used did not significantly influenced the final value. In conclusion, for the interpretation of ChE values it is important to get reference values in each laboratory and it is advantageous to prepare the reagents in the laboratory instead of purchasing commercial kits." @default.
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- W2609502575 date "2012-01-01" @default.
- W2609502575 modified "2023-09-27" @default.
- W2609502575 title "Comparación de tres métodos de determinación" @default.
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